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In JoVE (2)
- Microdissection av Black Widow Spider Silk-körtlar
- Syntetisk Spider Silk Production i laboratorieskala
Other Publications (1)
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Articles by Felicia Jeffery in JoVE
JoVE General
Microdissection av Black Widow Spider Silk-körtlar
Department of Biological Sciences, University of the Pacific
Här beskriver vi en effektiv strategi för att ta bort siden-producerande körtlar från buken av kvinnliga svarta änkan. Detta förfarande möjliggör en snabb isolering av de sju olika siden-körtlar i en mycket renat mode, en viktig process för utredarna att studera spindeltråd produktion och fiber montering.
JoVE Bioengineering
Syntetisk Spider Silk Production i laboratorieskala
Department of Biological Sciences, University of the Pacific
Trots de enastående mekaniska och biokemiska egenskaper hos spindel siden, kan detta material inte tas ut i stora mängder med konventionella medel. Här beskriver vi en effektiv strategi för att snurra regenatfibrer spindeltråd, vilket är en viktig process för utredarna studerar spindeltråd produktion och deras användning som nästa generations biomaterial.
Other articles by Felicia Jeffery on PubMed
Synthetic Spider Silk Fibers Spun from Pyriform Spidroin 2, a Glue Silk Protein Discovered in Orb-weaving Spider Attachment Discs
Spider attachment disc silk fibers are spun into a viscous liquid that rapidly solidifies, gluing dragline silk fibers to substrates for locomotion or web construction. Here we report the identification and artificial spinning of a novel attachment disc glue silk fibroin, Pyriform Spidroin 2 (PySp2), from the golden orb weaver Nephila clavipes . MS studies support PySp2 is a constituent of the pyriform gland that is spun into attachment discs. Analysis of the PySp2 protein architecture reveals sequence divergence relative to the other silk family members, including the cob weaver glue silk fibroin PySp1. PySp2 contains internal block repeats that consist of two subrepeat units: one dominated by Ser, Gln, and Ala and the other Pro-rich. Artificial spinning of recombinant PySp2 truncations shows that the Ser-Gln-Ala-rich subrepeat is sufficient for the assembly of polymeric subunits and subsequent fiber formation. These studies support that both orb- and cob-weaving spiders have evolved highly polar block-repeat sequences with the ability to self-assemble into fibers, suggesting a strategy to allow fiber fabrication in the liquid environment of the attachment discs.
