Florian Krammer Department of Microbiology Icahn School of Medicine at Mount Sinai Biography Publications Institution JoVE Articles Florian Krammer has not added a biography. If you are Florian Krammer and would like to personalize this page please email our Author Liaison for assistance. Publications Broadly Protective Murine Monoclonal Antibodies Against Influenza B Virus Target Highly Conserved Neuraminidase Epitopes Nature Microbiology. Aug, 2017 | Pubmed ID: 28827718 Synthetic TLR4 and TLR7 Ligands Work Additively Via MyD88 to Induce Protective Antiviral Immunity in Mice Journal of Virology. Jul, 2017 | Pubmed ID: 28724768 Is It Possible to Develop a "Universal" Influenza Virus Vaccine? Toward a Universal Influenza Virus Vaccine: Potential Target Antigens and Critical Aspects for Vaccine Development Cold Spring Harbor Perspectives in Biology. Jun, 2017 | Pubmed ID: 28663209 A Cross-clade H5N1 Influenza A Virus Neutralizing Monoclonal Antibody Binds to a Novel Epitope Within the Vestigial Esterase Domain of Hemagglutinin Antiviral Research. Aug, 2017 | Pubmed ID: 28633988 Novel Cross-Reactive Monoclonal Antibodies Against Ebolavirus Glycoproteins Show Protection in a Murine Challenge Model Journal of Virology. Aug, 2017 | Pubmed ID: 28592526 Stopping Emerging Influenza Viruses at Their Origin The Lancet. Infectious Diseases. Aug, 2017 | Pubmed ID: 28533095 An Amphibian Host Defense Peptide Is Virucidal for Human H1 Hemagglutinin-Bearing Influenza Viruses Immunity. Apr, 2017 | Pubmed ID: 28423338 Induction of Broadly Cross-Reactive Stalk-Specific Antibody Responses to Influenza Group 1 and Group 2 Hemagglutinins by Natural H7N9 Virus Infection in Humans The Journal of Infectious Diseases. 02, 2017 | Pubmed ID: 28380622 Enhancement of Zika Virus Pathogenesis by Preexisting Antiflavivirus Immunity Science (New York, N.Y.). Apr, 2017 | Pubmed ID: 28360135 Chimeric Hemagglutinin Constructs Induce Broad Protection Against Influenza B Virus Challenge in the Mouse Model Journal of Virology. Jun, 2017 | Pubmed ID: 28356526 Analysis of Anti-Influenza Virus Neuraminidase Antibodies in Children, Adults, and the Elderly by ELISA and Enzyme Inhibition: Evidence for Original Antigenic Sin MBio. Mar, 2017 | Pubmed ID: 28325769 A Novel Zika Virus Mouse Model Reveals Strain Specific Differences in Virus Pathogenesis and Host Inflammatory Immune Responses PLoS Pathogens. Mar, 2017 | Pubmed ID: 28278235 Defining the Antibody Cross-reactome Directed Against the Influenza Virus Surface Glycoproteins Nature Immunology. Apr, 2017 | Pubmed ID: 28192418 Progesterone-Based Contraceptives Reduce Adaptive Immune Responses and Protection Against Sequential Influenza A Virus Infections Journal of Virology. Apr, 2017 | Pubmed ID: 28179523 Influenza Virus-specific Antibody Dependent Cellular Cytoxicity Induced by Vaccination or Natural Infection Vaccine. Jan, 2017 | Pubmed ID: 27914742 Cross-Reactive and Cross-Neutralizing Activity of Human Mumps Antibodies Against a Novel Mumps Virus From Bats The Journal of Infectious Diseases. Jan, 2017 | Pubmed ID: 27811320 Molecular-level Analysis of the Serum Antibody Repertoire in Young Adults Before and After Seasonal Influenza Vaccination Nature Medicine. Dec, 2016 | Pubmed ID: 27820605 Epitope Specificity Plays a Critical Role in Regulating Antibody-dependent Cell-mediated Cytotoxicity Against Influenza A Virus Proceedings of the National Academy of Sciences of the United States of America. Oct, 2016 | Pubmed ID: 27698132 Canine H3N8 Influenza Vaccines Partially Protect Mice Against the Canine H3N2 Strain Currently Circulating in the United States Vaccine. Nov, 2016 | Pubmed ID: 27670073 Optimal Activation of Fc-mediated Effector Functions by Influenza Virus Hemagglutinin Antibodies Requires Two Points of Contact Proceedings of the National Academy of Sciences of the United States of America. Oct, 2016 | Pubmed ID: 27647907 Obesity Outweighs Protection Conferred by Adjuvanted Influenza Vaccination MBio. Aug, 2016 | Pubmed ID: 27486196 Both Neutralizing and Non-Neutralizing Human H7N9 Influenza Vaccine-Induced Monoclonal Antibodies Confer Protection Cell Host & Microbe. Jun, 2016 | Pubmed ID: 27281570 Globular Head-Displayed Conserved Influenza H1 Hemagglutinin Stalk Epitopes Confer Protection Against Heterologous H1N1 Virus PloS One. 2016 | Pubmed ID: 27088239 Broadly-Reactive Neutralizing and Non-neutralizing Antibodies Directed Against the H7 Influenza Virus Hemagglutinin Reveal Divergent Mechanisms of Protection PLoS Pathogens. Apr, 2016 | Pubmed ID: 27081859 Cryo-electron Microscopy Structures of Chimeric Hemagglutinin Displayed on a Universal Influenza Vaccine Candidate MBio. Mar, 2016 | Pubmed ID: 27006464 Novel Universal Influenza Virus Vaccine Approaches Current Opinion in Virology. Apr, 2016 | Pubmed ID: 26927813 New England Harbor Seal H3N8 Influenza Virus Retains Avian-like Receptor Specificity Scientific Reports. Feb, 2016 | Pubmed ID: 26888262 Age Dependence and Isotype Specificity of Influenza Virus Hemagglutinin Stalk-Reactive Antibodies in Humans MBio. Jan, 2016 | Pubmed ID: 26787832 Influenza A Viruses Expressing Intra- or Intergroup Chimeric Hemagglutinins Journal of Virology. Jan, 2016 | Pubmed ID: 26764009 Ion Efflux and Influenza Infection Trigger NLRP3 Inflammasome Signaling in Human Dendritic Cells Journal of Leukocyte Biology. May, 2016 | Pubmed ID: 26574023 Hemagglutinin Stalk Immunity Reduces Influenza Virus Replication and Transmission in Ferrets Journal of Virology. Dec, 2015 | Pubmed ID: 26719251 Vaccination with Vesicular Stomatitis Virus-Vectored Chimeric Hemagglutinins Protects Mice Against Divergent Influenza Virus Challenge Strains Journal of Virology. Dec, 2015 | Pubmed ID: 26676789 Immune History Profoundly Affects Broadly Protective B Cell Responses to Influenza Science Translational Medicine. Dec, 2015 | Pubmed ID: 26631631 Hemagglutinin Stalk- and Neuraminidase-Specific Monoclonal Antibodies Protect Against Lethal H10N8 Influenza Virus Infection in Mice Journal of Virology. Oct, 2015 | Pubmed ID: 26512088 The Quest for a Universal Flu Vaccine: Headless HA 2.0 Cell Host & Microbe. Oct, 2015 | Pubmed ID: 26468743 Anti-HA Glycoforms Drive B Cell Affinity Selection and Determine Influenza Vaccine Efficacy Cell. Jul, 2015 | Pubmed ID: 26140596 Hemagglutinin Receptor Binding of a Human Isolate of Influenza A(H10N8) Virus Emerging Infectious Diseases. Jul, 2015 | Pubmed ID: 26079843 Vaccination with Soluble Headless Hemagglutinin Protects Mice from Challenge with Divergent Influenza Viruses Vaccine. Jun, 2015 | Pubmed ID: 26026378 High Affinity Antibodies Against Influenza Characterize the Plasmablast Response in SLE Patients After Vaccination PloS One. 2015 | Pubmed ID: 25951191 Newcastle Disease Virus-Vectored H7 and H5 Live Vaccines Protect Chickens from Challenge with H7N9 or H5N1 Avian Influenza Viruses Journal of Virology. Jul, 2015 | Pubmed ID: 25926639 High-Affinity H7 Head and Stalk Domain-Specific Antibody Responses to an Inactivated Influenza H7N7 Vaccine After Priming With Live Attenuated Influenza Vaccine The Journal of Infectious Diseases. Oct, 2015 | Pubmed ID: 25838266 Vaccination with Adjuvanted Recombinant Neuraminidase Induces Broad Heterologous, but Not Heterosubtypic, Cross-protection Against Influenza Virus Infection in Mice MBio. Mar, 2015 | Pubmed ID: 25759506 Emerging Influenza Viruses and the Prospect of a Universal Influenza Virus Vaccine Biotechnology Journal. May, 2015 | Pubmed ID: 25728134 Advances in the Development of Influenza Virus Vaccines Nature Reviews. Drug Discovery. Mar, 2015 | Pubmed ID: 25722244 Preexisting Human Antibodies Neutralize Recently Emerged H7N9 Influenza Strains The Journal of Clinical Investigation. Mar, 2015 | Pubmed ID: 25689254 Domestic Pigs Are Susceptible to Infection with Influenza B Viruses Journal of Virology. May, 2015 | Pubmed ID: 25673727 An H10N8 Influenza Virus Vaccine Strain and Mouse Challenge Model Based on the Human Isolate A/Jiangxi-Donghu/346/13 Vaccine. Feb, 2015 | Pubmed ID: 25604801 Synthetic Toll-like Receptor 4 (TLR4) and TLR7 Ligands As Influenza Virus Vaccine Adjuvants Induce Rapid, Sustained, and Broadly Protective Responses Journal of Virology. Mar, 2015 | Pubmed ID: 25568203 A Viable Recombinant Rhabdovirus Lacking Its Glycoprotein Gene and Expressing Influenza Virus Hemagglutinin and Neuraminidase is a Potent Influenza Vaccine Journal of Virology. Mar, 2015 | Pubmed ID: 25540378 Advances in Universal Influenza Virus Vaccine Design and Antibody Mediated Therapies Based on Conserved Regions of the Hemagglutinin Current Topics in Microbiology and Immunology. 2015 | Pubmed ID: 25007847 Animal Models for Influenza Viruses: Implications for Universal Vaccine Development Pathogens (Basel, Switzerland). Oct, 2014 | Pubmed ID: 25436508 Characterization of a Broadly Neutralizing Monoclonal Antibody That Targets the Fusion Domain of Group 2 Influenza A Virus Hemagglutinin Journal of Virology. Dec, 2014 | Pubmed ID: 25210195 Induction of Broadly Reactive Anti-hemagglutinin Stalk Antibodies by an H5N1 Vaccine in Humans Journal of Virology. Nov, 2014 | Pubmed ID: 25210189 Induction of Broadly Cross-reactive Antibody Responses to the Influenza HA Stem Region Following H5N1 Vaccination in Humans Proceedings of the National Academy of Sciences of the United States of America. Sep, 2014 | Pubmed ID: 25157133 In the Shadow of Hemagglutinin: a Growing Interest in Influenza Viral Neuraminidase and Its Role As a Vaccine Antigen Viruses. Jun, 2014 | Pubmed ID: 24960271 An H7N1 Influenza Virus Vaccine Induces Broadly Reactive Antibody Responses Against H7N9 in Humans Clinical and Vaccine Immunology : CVI. Aug, 2014 | Pubmed ID: 24943383 Divergent H7 Immunogens Offer Protection from H7N9 Virus Challenge Journal of Virology. Apr, 2014 | Pubmed ID: 24453375 Assessment of Influenza Virus Hemagglutinin Stalk-based Immunity in Ferrets Journal of Virology. Mar, 2014 | Pubmed ID: 24403585 Universal Influenza Virus Vaccines: Need for Clinical Trials Nature Immunology. Jan, 2014 | Pubmed ID: 24352315 H3 Stalk-based Chimeric Hemagglutinin Influenza Virus Constructs Protect Mice from H7N9 Challenge Journal of Virology. Feb, 2014 | Pubmed ID: 24307585 One-shot Vaccination with an Insect Cell-derived Low-dose Influenza A H7 Virus-like Particle Preparation Protects Mice Against H7N9 Challenge Vaccine. Jan, 2014 | Pubmed ID: 24262313 Influenza A(H7N9) Virus Gains Neuraminidase Inhibitor Resistance Without Loss of in Vivo Virulence or Transmissibility Nature Communications. 2013 | Pubmed ID: 24326875 Adjuvants and Immunization Strategies to Induce Influenza Virus Hemagglutinin Stalk Antibodies PloS One. 2013 | Pubmed ID: 24223176 The Emergence of H7N9 Viruses: a Chance to Redefine Correlates of Protection for Influenza Virus Vaccines Expert Review of Vaccines. Oct, 2013 | Pubmed ID: 24160815 Influenza Virus Hemagglutinin Stalk-based Antibodies and Vaccines Current Opinion in Virology. Oct, 2013 | Pubmed ID: 23978327 H7N9 Influenza Viruses Interact Preferentially with α2,3-linked Sialic Acids and Bind Weakly to α2,6-linked Sialic Acids The Journal of General Virology. Nov, 2013 | Pubmed ID: 23950563 Neutralizing Antibodies Against Previously Encountered Influenza Virus Strains Increase over Time: a Longitudinal Analysis Science Translational Medicine. Aug, 2013 | Pubmed ID: 23946196 Induction of Cross-reactive Antibodies to Novel H7N9 Influenza Virus by Recombinant Newcastle Disease Virus Expressing a North American Lineage H7 Subtype Hemagglutinin Journal of Virology. Jul, 2013 | Pubmed ID: 23698299 Recombinant IgA is Sufficient to Prevent Influenza Virus Transmission in Guinea Pigs Journal of Virology. Jul, 2013 | Pubmed ID: 23698296 Purification of Infective Baculoviruses by Monoliths Journal of Chromatography. A. May, 2013 | Pubmed ID: 23587319 B Cell Response and Hemagglutinin Stalk-reactive Antibody Production in Different Age Cohorts Following 2009 H1N1 Influenza Virus Vaccination Clinical and Vaccine Immunology : CVI. Jun, 2013 | Pubmed ID: 23576673 Chimeric Hemagglutinin Influenza Virus Vaccine Constructs Elicit Broadly Protective Stalk-specific Antibodies Journal of Virology. Jun, 2013 | Pubmed ID: 23576508 H3N2 Influenza Virus Infection Induces Broadly Reactive Hemagglutinin Stalk Antibodies in Humans and Mice Journal of Virology. Apr, 2013 | Pubmed ID: 23408625 1976 and 2009 H1N1 Influenza Virus Vaccines Boost Anti-hemagglutinin Stalk Antibodies in Humans The Journal of Infectious Diseases. Jan, 2013 | Pubmed ID: 23087428 Residual Baculovirus in Insect Cell-derived Influenza Virus-like Particle Preparations Enhances Immunogenicity PloS One. 2012 | Pubmed ID: 23236516 A Carboxy-terminal Trimerization Domain Stabilizes Conformational Epitopes on the Stalk Domain of Soluble Recombinant Hemagglutinin Substrates PloS One. 2012 | Pubmed ID: 22928001 Hemagglutinin Stalk-Reactive Antibodies Are Boosted Following Sequential Infection with Seasonal and Pandemic H1N1 Influenza Virus in Mice Journal of Virology. Oct, 2012 | Pubmed ID: 22787225 NS1-truncated Live Attenuated Virus Vaccine Provides Robust Protection to Aged Mice from Viral Challenge Journal of Virology. Oct, 2012 | Pubmed ID: 22787224 Influenza Virus Infection in Guinea Pigs Raised As Livestock, Ecuador Emerging Infectious Diseases. Jul, 2012 | Pubmed ID: 22710350 A Pan-H1 Anti-hemagglutinin Monoclonal Antibody with Potent Broad-spectrum Efficacy in Vivo Journal of Virology. Jun, 2012 | Pubmed ID: 22491456 Attenuated Influenza Virus Construct with Enhanced Hemagglutinin Protein Expression Journal of Virology. May, 2012 | Pubmed ID: 22398291 Influenza Viruses Expressing Chimeric Hemagglutinins: Globular Head and Stalk Domains Derived from Different Subtypes Journal of Virology. May, 2012 | Pubmed ID: 22398287 Efficient Transmission of Pandemic H1N1 Influenza Viruses with High-level Oseltamivir Resistance Journal of Virology. May, 2012 | Pubmed ID: 22345446 Hemagglutinin Stalk Antibodies Elicited by the 2009 Pandemic Influenza Virus As a Mechanism for the Extinction of Seasonal H1N1 Viruses Proceedings of the National Academy of Sciences of the United States of America. Feb, 2012 | Pubmed ID: 22308500 Insect Cells for Antibody Production: Evaluation of an Efficient Alternative Journal of Biotechnology. May, 2011 | Pubmed ID: 21477625 Evaluation of the Influenza A Replicon for Transient Expression of Recombinant Proteins in Mammalian Cells PloS One. 2010 | Pubmed ID: 20949004 Alternative Influenza Vaccines Made by Insect Cells Trends in Molecular Medicine. Jul, 2010 | Pubmed ID: 20570562 Influenza Virus-like Particles As an Antigen-carrier Platform for the ESAT-6 Epitope of Mycobacterium Tuberculosis Journal of Virological Methods. Jul, 2010 | Pubmed ID: 20304011 Trichoplusia Ni Cells (High Five) Are Highly Efficient for the Production of Influenza A Virus-like Particles: a Comparison of Two Insect Cell Lines As Production Platforms for Influenza Vaccines Molecular Biotechnology. Jul, 2010 | Pubmed ID: 20300881 Swine-origin Pandemic H1N1 Influenza Virus-like Particles Produced in Insect Cells Induce Hemagglutination Inhibiting Antibodies in BALB/c Mice Biotechnology Journal. Jan, 2010 | Pubmed ID: 20041443 Generation of Escape Variants of Neutralizing Influenza Virus Monoclonal Antibodies Paul E. Leon1,2, Teddy John Wohlbold1,2, Wenqian He1,2, Mark J. Bailey1,2, Carole J. Henry3, Patrick C. Wilson3, Florian Krammer1, Gene S. Tan1 1Department of Microbiology, Icahn School of Medicine at Mount Sinai, 2Graduate School of Biomedical Sciences, Icahn School of Medicine at Mount Sinai, 3The Department of Medicine, Section of Rheumatology, The Knapp Center for Lupus and Immunology Research, The University of Chicago JoVE 56067 Immunology and Infection Expression of Functional Recombinant Hemagglutinin and Neuraminidase Proteins from the Novel H7N9 Influenza Virus Using the Baculovirus Expression System Irina Margine1,2, Peter Palese1,3, Florian Krammer1 1Department of Microbiology, Icahn School of Medicine at Mount Sinai, 2Graduate School of Biological Sciences, Icahn School of Medicine at Mount Sinai, 3Department of Medicine, Icahn School of Medicine at Mount Sinai JoVE 51112 Immunology and Infection
Generation of Escape Variants of Neutralizing Influenza Virus Monoclonal Antibodies Paul E. Leon1,2, Teddy John Wohlbold1,2, Wenqian He1,2, Mark J. Bailey1,2, Carole J. Henry3, Patrick C. Wilson3, Florian Krammer1, Gene S. Tan1 1Department of Microbiology, Icahn School of Medicine at Mount Sinai, 2Graduate School of Biomedical Sciences, Icahn School of Medicine at Mount Sinai, 3The Department of Medicine, Section of Rheumatology, The Knapp Center for Lupus and Immunology Research, The University of Chicago JoVE 56067 Immunology and Infection
Expression of Functional Recombinant Hemagglutinin and Neuraminidase Proteins from the Novel H7N9 Influenza Virus Using the Baculovirus Expression System Irina Margine1,2, Peter Palese1,3, Florian Krammer1 1Department of Microbiology, Icahn School of Medicine at Mount Sinai, 2Graduate School of Biological Sciences, Icahn School of Medicine at Mount Sinai, 3Department of Medicine, Icahn School of Medicine at Mount Sinai JoVE 51112 Immunology and Infection
