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In JoVE (1)
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Articles by Gemma Johnson in JoVE
JoVE Immunology and Infection
Detecção de aspergilose pulmonar invasiva em pacientes com doença hematológica maligna usando fluxo lateral Tecnologia
1Biosciences, University of Exeter, 2BICMS, Queen Mary University of London, 3St. Bartholomew's Hospital and The London NHS Trust
A rápida e precisa de teste ponto-of-care para a aspergilose pulmonar invasiva é apresentado. Leva vantagem de fluxo lateral-tecnologia, utilizando um anticorpo monoclonal específico que se liga a um
Other articles by Gemma Johnson on PubMed
Comparative Study of Diagnosis of PD Peritonitis by Quantitative Polymerase Chain Reaction for Bacterial DNA Vs Culture Methods
Peritonitis remains one of the main complications that afflict peritoneal dialysis patients. We conducted a pilot study to determine the feasibility and potential advantages of quantitative PCR (qPCR) assays for the presence of bacterial DNA in this clinical scenario.
Evaluation of BacLite Rapid MRSA, a Rapid Culture Based Screening Test for the Detection of Ciprofloxacin and Methicillin Resistant S. Aureus (MRSA) from Screening Swabs
Methicillin-resistant Staphylococcus aureus (MRSA) is a major nosocomial pathogen worldwide. The need for accurate and rapid screening methods to detect MRSA carriers has been clearly established. The performance of a novel assay, BacLite Rapid MRSA (Acolyte Biomedica, UK) for the rapid detection (5 h) and identification of hospital associated ciprofloxacin resistant strains of MRSA directly from nasal swab specimens was compared to that obtained by culture on Mannitol salt agar containing Oxacillin (MSAO) after 48 h incubation.
Vibrio Harveyi Sepsis in a Child with Cancer
A paediatric oncology patient presented with central line sepsis caused by Vibrio harveyi, a gram negative bioluminescent marine bacterium known to be pathogenic to fish and marine invertebrates, after swimming in the sea.
The Use of Molecular Techniques for Bacterial Detection in the Analysis of Gastric Aspirates Collected from Infants on the First Day of Life
Prospective service evaluation of the utility of molecular methods to analyse neonatal gastric aspirate specimens in a single neonatal unit and associated maternity unit. 43 newborn infants investigated for sepsis with median gestational age of 39 weeks (range 31-41 weeks) and median birth weight 3050 grams (range 1250-4220 g). Gastric aspirates routinely collected within 12h of birth were analysed using conventional and molecular methods for bacterial detection, bacterial DNA load and sequencing to identified bacterial species. RESULTS: Bacterial DNA loads varied from 0.03 to 1736 pg/microl of DNA extract (1 microl of DNA extract equivalent to 4 microl gastric aspirate). Bacteria were identified in 30/43 (70%) of samples by molecular methods and 10/43 (23.3%) of samples by culture. Cultures were only positive when the bacterial DNA exceeded 4.5 pg/microl of extract. Infants with prolonged rupture of membranes (>24h prior to delivery) had a DNA load on average 23 times higher than those without (95%CI 3.7 to 141; p=0.001). Additional bacteria detected by molecular methods included many species that are fastidious and potentially pathogenic including Leptotrichia spp., Serratia spp., Ureaplasma spp., Veillonella spp., Haemophilus influenzae and Group B Streptococcus. Due to a low rate of adverse outcomes it was not possible to correlate bacterial identifications or DNA load with infant outcome. CONCLUSIONS: Molecular methods can identify bacteria from a greater proportion of gastric aspirate specimens that conventional culture. Further work is required to establish whether this information can be used to improve infant outcomes.
Impact of the Revised (2008) EORTC/MSG Definitions for Invasive Fungal Disease on the Rates of Diagnosis of Invasive Aspergillosis
Diagnosis of invasive aspergillosis (IA) remains a challenge as the clinical manifestations are not specific, and a histological diagnosis is often unfeasible. The 2002 European Organization for Research and Treatment of Cancer (EORTC) and the National Institute of Allergy and Infectious Diseases Mycoses Study Group (MSG) criteria for classification of cases into possible, probable or proven were revised in 2008. Our objective was to analyze the impact of these revisions on the diagnosis of IA. A retrospective analysis of 589 high risk patient-episodes revealed that 125 of 155 ?possible? (81%) and 12 of 16 ?probable? (75%) cases of IA should be changed to ?non-classifiable? when the new criteria were applied. We concluded, as expected, that the 2008 EORTC/MSG revised definitions reduced the number of cases classified as ?possible? IA, but additionally, there has been a dramatic reduction in ?probable? cases. These changes have significant implications on the interpretation of clinical trial data based on EORTC/MSG classifications.
