Articles by Harry W. Read in JoVE

GC-based Detection of Aldononitrile Acetate Derivatized Glucosamine and Muramic Acid for Microbial Residue Determination in Soil

JoVE 3767 5/19/2012

Chao Liang^1,2, Harry W. Read², Teri C. Balser^2,3

¹DOE-Great Lakes Bioenergy Research Center, University of Wisconsin, Madison, ²Department of Soil Science, University of Wisconsin, Madison, ³Department of Soil and Water Science, University of Florida

We describe a method protocol for the GC-based analysis of the aldonitrile acetate derivatives of glucosamine and muramic acid extracted from soil. For elucidation of the chemical mechanism, we also present a strategy to confirm the structure of the derivative and the ion fragments formed upon electron ionization.