Articles by Heryet Cameron in JoVE

DNA Extraction from Paraffin Embedded Material for Genetic and Epigenetic Analyses

JoVE 2763 3/26/2011

Larissa A. Pikor*^1,2, Katey S. S. Enfield*^1,2, Heryet Cameron³, Wan L. Lam^1,2,4

¹Department of Integrative Oncology, BC Cancer Research Centre, ²Interdisciplinary Oncology Program, University of British Columbia - UBC, ³Photography/Video Production, Multi-Media Services, BC Cancer Agency, ⁴Department of Pathology and Laboratory Medicine, University of British Columbia - UBC

This video demonstrates the protocol for DNA extraction from formalin-fixed paraffin-embedded material. This is a multi-day procedure in which tissue sections are deparaffinized with xylene, rehydrated with ethanol and treated with proteinase K to purify and isolate DNA for subsequent gene-specific or genome-wide analysis.