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In JoVE (1)
Other Publications (12)
- Taehan Ch'ikkwa Uisa Hyŏphoe Chi
- [Zasshi] [Journal]. Nihon Kyōbu Geka Gakkai
- Taehan Ch'ikkwa Uisa Hyŏphoe Chi
- Journal of Biochemistry
- Taehan Ch'ikkwa Uisa Hyŏphoe Chi
- Plant Molecular Biology
- Diagnostic and Therapeutic Endoscopy
- European Journal of Cardio-thoracic Surgery : Official Journal of the European Association for Cardio-thoracic Surgery
- The Japanese Journal of Thoracic and Cardiovascular Surgery : Official Publication of the Japanese Association for Thoracic Surgery = Nihon Kyōbu Geka Gakkai Zasshi
- Zhonghua Er Ke Za Zhi. Chinese Journal of Pediatrics
- Oncology Nursing Forum
- Protein Expression and Purification
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Articles by Ilídio J. Correia in JoVE
JoVE General
Isolamento da Human Umbilical células musculares arteriais (HUASMC)
Os cordões umbilicais são usados para isolar as células do músculo liso por caminhos diferentes. Neste trabalho utilizou-se o tratamento enzimático de células musculares lisas isoladas.
Other articles by Ilídio J. Correia on PubMed
[Obstruction Syndrome of the Right Ventricle; Re-evaluation of the Obstructive Malformations in the Right Ventricle, Especially of the Two-chambered Right Ventricle (author's Transl)]
Crystal Structures of Modified Myoglobins. I. Heme Orientation and Structural Changes Around Heme in Myoglobins Reconstituted with Isopemptoheme, Pemptoheme, 2-ethyldeuteroheme, and 4-ethyldeuteroheme
The crystal structures of sperm whale metmyoglobins reconstituted with four modified hemes, isopemptoheme, pemptoheme, 2-ethyldeuteroheme, and 4-ethyldeuteroheme, have been determined and refined at 2.2 A resolution to R = 0.217, 0.218, 0.213, and 0.222, respectively. All the crystals of these myoglobins are isomorphous with that of native metmyoglobin. The structural changes of the modified myoglobin from the native myoglobin were examined on difference Fourier maps; the orientation of 4-ethyldeuteroheme in the heme pocket is such that the heme is rotated by 180 degrees about an axis through the alpha-gamma-meso carbons, whereas the orientations of the other three hemes are the same as that of the protoheme in the native myoglobin. The changes of the structures around the heme become greater in the order of isopemptoheme, 2-ethyldeuteroheme less than pemptoheme less than 4-ethyldeuteroheme. The magnitudes of the changes seem to be related to the oxygen affinities of these four reconstituted myoglobins.
[A Clinical Study of Oral Adenoid Cystic Carcinoma]
Author experienced a case of tubular type, and a case of solid & basaloid type in adenoid cystic carcinoma and presented these cases through the review of literature. The paralysis was found. Both cases showed perineural, muscular tissue and bony invasion. Respectively hemimaxillectomy, and subtotal maxillectomy were operated. The origin of adenoid cystic carcinoma was considered as myoepithelial cell combined with intercalated duct reserve cell.
Phenotypically Normal Transgenic T-cyt Tobacco Plants As a Model for the Investigation of Plant Gene Expression in Response to Phytohormonal Stress
The tumour-inducing T-DNA gene 4 (T-cyt gene) of the nopaline Ti plasmid pTiC58 was cloned and introduced into tobacco cells by leaf disc transformation using Agrobacterium plasmid vectors. Tobacco shoots exposed to elevated cytokinin levels were unable to develop roots and lacked apical dominance. Using exogenously applied phytohormone manipulations we were able to regenerate morphologically normal transgenic tobacco plants which differed in endogenous cytokinin levels from normal untransformed plants. Although T-cyt gene mRNA levels, as revealed by dot-blot hybridization data, in these rooting plants were only about half those in primary transformed shoots the total amount of cytokinins was much lower than in crown gall tissue or cytokinin-type transformed shoots as reported by others. Nevertheless the cytokinin content in T-cyt plants was about 3 times greater than in control tobacco plants. Elevated cytokinin levels have been shown to change the expression of several plant genes, including some nuclear genes encoding chloroplast proteins. Our results show that the mRNA levels of chloroplast rbcL gene increase in cytokinin-type transgenic tobacco plants as compared with untransformed plants. Data obtained suggest that T-cyt transgenic plants are a good model for studying plant gene activity in different parts of the plant under endogenous cytokinin stress.
Early Detection of Bronchial Lesions Using System of Autofluorescence Endoscopy (SAFE) 1000
Recently several endoscopic fluorescence detection systems have been developed. In some of them, laser light was used for the excitation of autofluorescence, and sophisticated techniques were also necessary to amplify the fluorescence signal as well.The result of fluorescence diagnosis using a simple system with a conventional Xenon lamp excitation and an image intensifier is reported. The respective results of sensitivity and positive predictive values of cancer plus dysplasia were 66%, and 62% by standard bronchoscopy and 92% and 88% by the newly developed autofluorescence system. In this paper, developed endoscope for detection of tissue/mucosal autofluorescence without the application of any photosensitizing agents or use of any lasers is evaluated.
Surgical Repair of Coronary Arteriovenous Fistula: a Simple and Useful Approach to Identify the Fistulous Communication
In repairing coronary arteriovenous fistula (CAVF), it is very important to interrupt the fistulous tract without compromise of normal coronary vessel flow. In our case, selective coronary arteriography showed that the CAVF from the left anterior descending coronary artery (LAD) was very close to the native coronary artery and had a very broad and short neck. We describe a simple and useful approach, by using both antegrade and retrograde coronary perfusion, that makes it possible to certainly protect myocardium and to clearly distinguish the normal native coronary artery from the fistulous tract.
Simultaneous Operation of off Pump Coronary Artery Bypass and Abdominal Aortic Aneurysm Repair
Coronary artery disease (CAD) and abdominal aortic aneurysms (AAA) commonly coexist. However, each disease treatment complicates the management of the other. In this study, we evaluate whether a simultaneous operation of AAA repair and off pump coronary artery bypass (OPCAB) would be safe and acceptable, compared with either procedure alone.
[Comparison of the Survival Rate and the Percentage of Apoptosis Between Eosinophils from Bronchoalveolar Lavage Fluid and Peripheral Blood in Asthmatic Guinea Pig Model]
Quality of Life and Sociodemographic Characteristics of Patients with Cancer in Turkey
To determine whether a relationship exists between quality of life (QOL) and the sociodemographic characteristics of gender, age, marital status, educational level, occupation, and level of income in patients with cancer in Turkey.
High-level Secretory Expression, Purification and Characterization of Ailuropoda Melanoleuca Growth Hormone in Pichia Pastoris
Growth hormone is one of the most important hormones, which is involved in many reproductive processes of giant panda Ailuropoda melanoleuca. In this study, the mature peptide of A. melanoleuca growth hormone (AmGH) was successfully expressed and secreted in Pichia pastoris under the control of AOX1 promoter. The expression condition for AmGH in P. pastoris, such as the expression time, pH value and methanol concentration in the BMMY were optimized and the AmGH expression level is about 100 mg/L using GS115 recombinant under optimized condition (96 h of 1.5% methanol induction). The secreted nascent AmGH were purified using ammonium sulfate fractionation. The mature AmGH protein exhibited a molecular mass of approximately 22 kDa on SDS-PAGE. This study would provide a new opportunity for large-scale expression and purification of AmGH, which might facilitate studies on the biological activity of AmGH.
