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Articles by IngMarie Olsson in JoVE
JoVE General
Productie van Weefsel Microarrays, Immunohistochemie vlekken en Digitalisering Binnen het menselijke eiwit Atlas
Department of Immunology, Genetics and Pathology, Science for Life Laboratory, Uppsala University
Weefsel microarrays zorgt voor een efficiënte methode om gelijktijdige informatie te verkrijgen uit een veelheid van weefsels. Representatieve delen van weefsels worden samengevoegd tot een paraffineblok. Secties van het blok voor immunohistochemie en analyse van eiwit expressie. Digitaal scannen genereert corresponderende beelden voor de distributie van gegevens.
Other articles by IngMarie Olsson on PubMed
A Human Protein Atlas for Normal and Cancer Tissues Based on Antibody Proteomics
Antibody-based proteomics provides a powerful approach for the functional study of the human proteome involving the systematic generation of protein-specific affinity reagents. We used this strategy to construct a comprehensive, antibody-based protein atlas for expression and localization profiles in 48 normal human tissues and 20 different cancers. Here we report a new publicly available database containing, in the first version, approximately 400,000 high resolution images corresponding to more than 700 antibodies toward human proteins. Each image has been annotated by a certified pathologist to provide a knowledge base for functional studies and to allow queries about protein profiles in normal and disease tissues. Our results suggest it should be possible to extend this analysis to the majority of all human proteins thus providing a valuable tool for medical and biological research.
