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In JoVE (2)
- Preparation of Single-Cell Suspensions from Mouse Spleen with the gentleMACS Dissociator
- Standardized Preparation of Single-Cell Suspensions from Mouse Lung Tissue using the gentleMACS Dissociator
Other Publications (0)
Articles by Irene Zehnter in JoVE
JoVE General
Preparation of Single-Cell Suspensions from Mouse Spleen with the gentleMACS Dissociator
This video describes a simple, time-saving technique for automated tissue dissociation using the gentleMACS Dissociator to prepare single-cell suspensions of mouse splenocytes.
JoVE General
Standardized Preparation of Single-Cell Suspensions from Mouse Lung Tissue using the gentleMACS Dissociator
Dissociating cells from specific tissue types requires specific parameters for tissue agitation to obtain a high volume of viable, culturable cells. The Miltenyi gentleMACS dissociator optimizes this task with a simple, practical protocol. In this publication the use of this apparatus on lung tissue is explained.
