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In JoVE (1)
Other Publications (2)
Articles by Irina Shats in JoVE
Derivation of Glial Restricted Precursors from E13 mice
André W. Phillips1,2, Sina Falahati1,2, Roshi DeSilva1,3, Irina Shats2, Joel Marx1, Edwin Arauz1, Douglas A. Kerr4, Jeffrey D. Rothstein2,5, Michael V. Johnston1,2,6, Ali Fatemi1,2,6
1Hugo W. Moser Research Institute at Kennedy Krieger, Johns Hopkins University, 2Department of Neurology, Johns Hopkins School of Medicine, 3University of Maryland, 4Experimental Neurology, Biogen Idec, 5The Brain Science Institute, Johns Hopkins School of Medicine, 6Department of Pediatrics, Johns Hopkins School of Medicine
This protocol outlines the derivation of Glial Restricted Precursors from fetal spinal cords and maintained in vitro either for transplantation or for the study of oligodendrocytic lineage.
Other articles by Irina Shats on PubMed
Axonal Growth of Embryonic Stem Cell-derived Motoneurons in Vitro and in Motoneuron-injured Adult Rats
Proceedings of the National Academy of Sciences of the United States of America. May, 2004 | Pubmed ID: 15118094
We generated spinal motoneurons from embryonic stem (ES) cells to determine the developmental potential of these cells in vitro and their capacity to replace motoneurons in the adult mammalian spinal cord. ES cell-derived motoneurons extended long axons, formed neuromuscular junctions, and induced muscle contraction when cocultured with myoblasts. We transplanted motoneuron-committed ES cells into the spinal cords of adult rats with motoneuron injury and found that approximately 3,000 ES cell-derived motoneurons (25% of input) survived for >1 month in the spinal cord of each animal. ES cell-derived axonal growth was inhibited by myelin, and this inhibition was overcome by administration of dibutyryl cAMP (dbcAMP) or a Rho kinase inhibitor in vitro and in vivo. In transplanted rats infused with dbcAMP, approximately 80 ES cell-derived motor axons were observed within the ventral roots of each animal, whereas none were observed in transplanted rats not treated with dbcAMP. Because these cells replicate many of the developmental and mature features of true motoneurons, they are an important biological tool to understand formation of motor units in vitro and a potential therapeutic tool to reconstitute neural circuits in vivo.
Annals of Neurology. Jul, 2006 | Pubmed ID: 16802299
We explored the potential of embryonic stem cell-derived motor neurons to functionally replace those cells destroyed in paralyzed adult rats.