Articles by Irmgard Hoppe in JoVE

Implantation of a Carotid Cuff for Triggering Shear-stress Induced Atherosclerosis in Mice

JoVE 3308 1/13/2012

Michael T. Kuhlmann¹, Simon Cuhlmann^2,3, Irmgard Hoppe¹, Rob Krams³, Paul C. Evans², Gustav J. Strijkers⁴, Klaas Nicolay⁴, Sven Hermann¹, Michael Schäfers¹

¹European Institute for Molecular Imaging, Westfälische Wilhelms-University Münster, ²British Heart Foundation Cardiovascular Sciences Unit, Imperial College London, ³Department of Bioengineering, Imperial College London, ⁴Biomedical Engineering, Eindhoven University of Technology

The constricting cuff presented in this article is designed to induce atherosclerosis in the murine common carotid artery. Due to the conical shape of its inner lumen the implanted cuff generates well-defined regions of low, high and oscillatory shear stress triggering the development of atherosclerotic lesions of different inflammatory phenotypes.