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In JoVE (1)
Other Publications (1)
Articles by Ivan Liadi in JoVE
Quantitative High-throughput Single-cell Cytotoxicity Assay For T Cells
Ivan Liadi1, Jason Roszik2, Gabrielle Romain1, Laurence J.N. Cooper2, Navin Varadarajan1
1Department of Chemical and Biomolecular Engineering, University of Houston, 2Division of Pediatrics, Research Unit 907, University of Texas MD Anderson Cancer Center
We describe a single-cell high-throughput assay to measure cytotoxicity of T cells when incubated with tumor target cells. This method employs a dense, elastomeric array of sub-nanoliter wells (~100,000 wells/array) to spatially confine the T cells and target cells at defined ratios and is coupled to fluorescence microscopy to monitor effector-target conjugation and subsequent apoptosis.
Published February 2, 2013. Keywords: Cancer Biology, Immunology, Cellular Biology, Molecular Biology, Medicine, Chemical Engineering, Biomolecular Engineering, Bioengineering, Immunotherapy, Adoptive, Microfluidics, Nanowell arrays, PDMS, BioStation, T Cells, tumor target cells, labeling, cytotoxicity, microscopy, assay
Other articles by Ivan Liadi on PubMed
Nigerian Journal of Medicine : Journal of the National Association of Resident Doctors of Nigeria. Oct-Dec, 2006 | Pubmed ID: 17111735
Vaginal fistulae particularly vesico-vagina fistula, is a common urogynaecologic problem in Sub-Saharan Africa. Majority of these cases are acquired and congenital fistulas are rare. We here report a case of congenital vaginal fistula secondary to an ectopic ureter in 15 year old Nigerian female.