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Articles by Ivan Liadi in JoVE

 JoVE Immunology and Infection

Quantitative High-throughput Single-cell Cytotoxicity Assay For T Cells


JoVE 50058 2/02/2013

1Department of Chemical and Biomolecular Engineering, University of Houston, 2Division of Pediatrics, Research Unit 907, University of Texas MD Anderson Cancer Center

We describe a single-cell high-throughput assay to measure cytotoxicity of T cells when incubated with tumor target cells. This method employs a dense, elastomeric array of sub-nanoliter wells (~100,000 wells/array) to spatially confine the T cells and target cells at defined ratios and is coupled to fluorescence microscopy to monitor effector-target conjugation and subsequent apoptosis.

Other articles by Ivan Liadi on PubMed

Congenital Vaginal Fistula from a Single System Ectopic Ureter: A Case Report

Vaginal fistulae particularly vesico-vagina fistula, is a common urogynaecologic problem in Sub-Saharan Africa. Majority of these cases are acquired and congenital fistulas are rare. We here report a case of congenital vaginal fistula secondary to an ectopic ureter in 15 year old Nigerian female.

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