We describe a single-cell high-throughput assay to measure cytotoxicity of T cells when incubated with tumor target cells. This method employs a dense, elastomeric array of sub-nanoliter wells (~100,000 wells/array) to spatially confine the T cells and target cells at defined ratios and is coupled to fluorescence microscopy to monitor effector-target conjugation and subsequent apoptosis.
Nigerian Journal of Medicine : Journal of the National Association of Resident Doctors of Nigeria. Oct-Dec, 2006 | Pubmed ID: 17111735
Vaginal fistulae particularly vesico-vagina fistula, is a common urogynaecologic problem in Sub-Saharan Africa. Majority of these cases are acquired and congenital fistulas are rare. We here report a case of congenital vaginal fistula secondary to an ectopic ureter in 15 year old Nigerian female.