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In JoVE (2)
- मानव नाल की नस endothelial कोशिकाओं के अलगाव (HUVEC)
- Angiogenesis के अध्ययन के लिए अनुकूलित आतंच जेल मनका परख
Other Publications (2)
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Articles by Jaeger Davis in JoVE
JoVE General
मानव नाल की नस endothelial कोशिकाओं के अलगाव (HUVEC)
Department of Molecular Biology and Biochemistry, University of California, Irvine (UCI)
यह वीडियो प्रोटोकॉल और मानव मानव गर्भनाल से नाल की नस endothelial कोशिकाओं (HUVEC) के अलगाव की संस्कृति को दिखाता है. एक बार अलग इन विट्रो अनुकूलित आतंच जेल मनका भी ह्यूजेस प्रयोगशाला द्वारा प्रदर्शन परख की तरह angiogenesis assays में इन कोशिकाओं के लिए इस्तेमाल किया जा सकता है.
JoVE General
Angiogenesis के अध्ययन के लिए अनुकूलित आतंच जेल मनका परख
Department of Molecular Biology and Biochemistry, University of California, Irvine (UCI)
इस वीडियो इन विट्रो angiogenesis परख में एक के प्रोटोकॉल को दर्शाता है कि angiogenesis के कई चरणों का स्मरण दिलाता है. अंकुरण के समय चूक छवियों, लुमेन गठन, शाखाओं में बंटी और सम्मिलन - angiogenesis की प्रमुख विशेषताएं - दिखाए जाते हैं.
Other articles by Jaeger Davis on PubMed
TNF Primes Endothelial Cells for Angiogenic Sprouting by Inducing a Tip Cell Phenotype
Pathological angiogenesis associated with wound healing often occurs subsequent to an inflammatory response that includes the secretion of cytokines such as tumor necrosis factor (TNF). Controversy exists on the angiogenic actions of TNF, with it being generally proangiogenic in vivo, but antiangiogenic in vitro. We find that whereas continuous administration of TNF in vitro or in vivo inhibits angiogenic sprouting, a 2- to 3-day pulse stimulates angiogenesis by inducing an endothelial "tip cell" phenotype. TNF induces the known tip cell genes platelet-derived growth factor B (PDGFB) and vascular endothelial cell growth factor receptor-2 (VEGFR2), while at the same time blocking signaling through VEGFR2, thus delaying the VEGF-driven angiogenic response. Notch signaling regulates tip cell function, and we find that TNF also induces the notch ligand jagged-1, through an NFkappaB-dependent mechanism. Enrichment of jagged-1 in tip cells was confirmed by immunofluorescent staining as well as by laser capture microdissection/quantitative reverse-transcription-polymerase chain reaction (qRT-PCR) of tip cells sprouting in vitro. Thus, in angiogenesis, the temporal expression of TNF is critical: it delays angiogenesis initially by blocking signaling through VEGFR2, but in addition by inducing a tip cell phenotype through an NFkappaB-dependent pathway, it concomitantly primes endothelial cells (ECs) for sprouting once the initial inflammatory wave has passed.
Cdkn2a is an Atherosclerosis Modifier Locus That Regulates Monocyte/macrophage Proliferation
Common genetic variants in a 58-kb region of chromosome 9p21, near the CDKN2A/CDKN2B tumor suppressor locus, are strongly associated with coronary artery disease. However, the underlying mechanism of action remains unknown.
