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In JoVE (1)
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Articles by Jeffrey D. Konowalchuk in JoVE
JoVE General
Измененный Аннексин В / пропидия йодидом Апоптоз Пробирной для точной оценки клеточной смерти
1Department of Biological Sciences, University of Alberta, 2Department of Agriculture, Food and Nutrition Sciences, University of Alberta
Точным методом оценки гибели клеток описывается. Протокол улучшает обычных Аннексин В / пропидий йодида (PI) протоколов, которые отображают до 40% ложно-положительных событий в клеточных линий и первичных клеток от широкого круга животных моделях.
Other articles by Jeffrey D. Konowalchuk on PubMed
MUC1 Mucin is Expressed on Human T-regulatory Cells: Function in Both Co-stimulation and Co-inhibition
MUC1 mucin, an important protein of epithelial cells and epithelial-derived carcinomas, is also expressed on activated T cells, showing both positive and negative regulatory functions. It is currently unknown whether MUC1 is a true regulatory protein of T cells and what conditions lead to MUC1 co-stimulation versus co-inhibition. We have found that MUC1 is expressed on the majority of T-regulatory cells (CD4(+)/CD25(+)/FoxP3(+)) in humans (>90%) and that CD3/MUC1 co-stimulation leads to an increased number of T-regulatory cells. We also discovered that the immunoregulatory function is dependent upon the number of accessory (CD3(-)) cells present, with co-inhibition occurring with <5-10% accessory cells while co-stimulation begins with a reconstitution of ~50% accessory cells. Co-inhibition was also found to not be the result of the apoptosis but a separate and unknown pathway. This data further characterizes MUC1 as an immunoregulatory protein of T cells capable of giving a positive or negative stimulus.
