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In JoVE (2)
- A high-throughput method to globally study the organelle morphology in S. cerevisiae
- Identification of protein complexes with quantitative proteomics in S. cerevisiae
Other Publications (0)
Articles by Jesse Tzu-Cheng Chao in JoVE
JoVE General
A high-throughput method to globally study the organelle morphology in S. cerevisiae
Department of Cellular and Physiological Sciences, University of British Columbia - UBC
GFP-fusion proteins are widely used to visualize organelles by confocal microscopy. However, screening for mutations that affect the morphology of organelles generally requires individual mutagenesis and is time consuming. Here, we demonstrate a method to simultaneously incorporate organelle-GFP markers in almost 5,000 non-essential genes in yeast.
JoVE General
Identification of protein complexes with quantitative proteomics in S. cerevisiae
1Department of Cellular and Physiological Sciences, University of British Columbia - UBC, 2Department of Biochemistry and Molecular Biology, University of British Columbia - UBC
Here we describe a new quantitative proteomics technique for identifying protein complexes in Saccharomyces cerevisiae. In this study, we have used the SILAC method together with affinity purification followed by tandem mass spectrometry to identify with high specificity the binding partners of an ER protein, Scs2p.
