The Journal of Visualized Experiments (JoVE) is a peer reviewed, PubMed-indexed video journal. Our mission is to increase the productivity of scientific research.

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Articles by Jesse Tzu-Cheng Chao in JoVE

 JoVE General

一种高通量的方法,在全球范围内研究在酿酒酵母中的细胞器形态


JoVE 1224 3/02/2009

Department of Cellular and Physiological Sciences, University of British Columbia - UBC

GFP融合蛋白被广泛应用于共聚焦显微镜可视化的细胞器。然而,筛选突变,影响细胞器的形态一般需要个人的突变,非常耗时。在这里,我们展示了一种方法,同时结合近5000个非必要基因在酵母细胞器- GFP标记。

 JoVE General

鉴定与定量蛋白质组学在酿酒酵母中的蛋白质复合物


JoVE 1225 3/04/2009

1Department of Cellular and Physiological Sciences, University of British Columbia - UBC, 2Department of Biochemistry and Molecular Biology, University of British Columbia - UBC

在这里,我们描述了一个新的量化确定在酿酒酵母中的蛋白质复合物的蛋白质组学技术。在这项研究中,我们一起串联质谱法,具有高特异性识别ER蛋白,Scs2p的具有约束力的合作伙伴的亲和纯化使用的SILAC方法。

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