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In JoVE (1)
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Articles by Jesse Barnes in JoVE
JoVE Neuroscience
Um método geral para a avaliação de Incubação de Craving Sacarose em Ratos
Department of Psychology and Program in Behavioral Neuroscience, Western Washington University
Responder por sinais de alimentos ou drogas emparelhados aumenta ao longo de um período de abstinência e esta pode estar relacionada com uma susceptibilidade aumentada a comportamentos recaída. Aqui detalhamos um procedimento para avaliar este "incubação de desejo" em ratos que têm auto-administrado de sacarose.
Other articles by Jesse Barnes on PubMed
Aggregation of Granulocyte-colony Stimulating Factor in Vitro Involves a Conformationally Altered Monomeric State
Aggregation of partially folded intermediates populated during protein folding processes has been described for many proteins. Likewise, partially unfolded chains, generated by perturbation of numerous proteins by heat or chemical denaturants, have also been shown to aggregate readily. However, the process of protein aggregation from native-state conditions is less well understood. Granulocyte-colony stimulating factor (G-CSF), a member of the four-helix bundle class of cytokines, is a therapeutically relevant protein involved in stimulating the growth and maturation of phagocytotic white blood cells. Under native-like conditions (37 degrees C [pH 7.0]), G-CSF shows a significant propensity to aggregate. Our data suggest that under these conditions, native G-CSF exists in equilibrium with an altered conformation, which is highly aggregation prone. This species is enriched in 1-2 M GdmCl, as determined by tryptophan fluorescence and increased aggregation kinetics. In particular, specific changes in Trp58 fluorescence report a local rearrangement in the large loop region between helices A and B. However, circular dichroism, reactivity toward cyanylation, and ANS binding demonstrate that this conformational change is subtle, having no substantial disruption of secondary and tertiary structure, reactivity of the free sulfhydryl at Cys17 or exposure of buried hydrophobic regions. There is no indication that this altered conformation is important to biological activity, making it an attractive target for rational protein stabilization.
Effects of Systemic or Nucleus Accumbens-directed Dopamine D1 Receptor Antagonism on Sucrose Seeking in Rats
Conditioned cues can elicit relapse to drug- and food-seeking behavior over prolonged periods of abstinence. If seeking behavior depends on mesolimbic dopamine D1 receptors, blocking these receptors should reduce seeking behavior.
