Articles by Jose Ruiz Ramirez in JoVE

Protocol for Dengue Infections in Mosquitoes (A. aegypti) and Infection Phenotype Determination

JoVE 220 7/04/2007

Suchismita Das, Lindsey Garver, Jose Ruiz Ramirez, Zhiyong Xi, George Dimopoulos

Malaria Research Institute, Bloomberg School of Public Health, Johns Hopkins University

Once a gene is identified as potentially refractory for the dengue virus, it must be evaluated for it's role in preventing viral infections within the mosquito. This protocol illustrates how the extent of dengue infections of mosquitoes can be assayed. The techniques for growing up the virus in culture, membrane feeding mosquitoes human blood, and assaying viral titers in the mosquito midgut are demonstrated.