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In JoVE (1)
Other Publications (1)
Articles by Joshua N. Milstein in JoVE
Stretching Short Sequences of DNA with Constant Force Axial Optical Tweezers
Krishnan Raghunathan1, Joshua N. Milstein2, Jens -Christian Meiners2
1LSA Biophysics, University of Michigan, 2LSA Biophysics, Department of Physics, University of Michigan
We illustrate the use of a constant force axial optical tweezers to explore the mechanical properties of short DNA molecules. By stretching DNA axially, we minimize steric hindrances and artifacts arising in conventional lateral manipulation, allowing us to study DNA molecules as short as ~100 nm.
Other articles by Joshua N. Milstein on PubMed
Dynamic Moment Analysis of the Extracellular Electric Field of a Biologically Realistic Spiking Neuron
Neural Computation. Aug, 2008 | Pubmed ID: 18386982
Based on the membrane currents generated by an action potential in a biologically realistic model of a pyramidal, hippocampal cell within rat CA1, we perform a moment expansion of the extracellular field potential. We decompose the potential into both inverse and classical moments and show that this method is a rapid and efficient way to calculate the extracellular field both near and far from the cell body. The action potential gives rise to a large quadrupole moment that contributes to the extracellular field up to distances of almost 1 cm. This method will serve as a starting point in connecting the microscopic generation of electric fields at the level of neurons to macroscopic observables such as the local field potential.