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In JoVE (2)
- Сегментация и измерение объемов жира в мышиной модели ожирения Использование рентгеновской компьютерной томографии
- 3D печать Доклинические рентгеновской компьютерной томографии наборов данных
Other Publications (2)
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Articles by Justin M. Diener in JoVE
JoVE Clinical and Translational Medicine
Сегментация и измерение объемов жира в мышиной модели ожирения Использование рентгеновской компьютерной томографии
1Carestream Molecular Imaging, 2Department of Chemistry and Biochemistry, University of Notre Dame, 3Freimann Life Science Center, University of Notre Dame, 4Research and Development, Oncovision, GEM-Imaging S.A.
Жир контент-анализ регулярно проводится в исследованиях с использованием мышиной модели ожирения. Новые методы в маленькое животное КТ и анализ обеспечения продольной подробно богатых жиром контент-анализ. Здесь мы подробно шаг за шагом процедуры для выполнения мелких животных КТ, анализа и визуализации.
JoVE General
3D печать Доклинические рентгеновской компьютерной томографии наборов данных
1Department of Chemistry and Biochemistry, University of Notre Dame, 2Freimann Life Science Center, University of Notre Dame, 3Department of Biological Sciences, University of Notre Dame, 4Notre Dame Integrated Imaging Facility, University of Notre Dame, 5MakerBot Industries LLC, 6Departments of Biological Sciences, Aerospace and Mechanical Engineering, and Anthropology, University of Notre Dame, 7Harper Cancer Research Institute, University of Notre Dame
Использование современных экструзионных пластиковых и технологии печати, теперь это возможно быстро и недорого производить физические модели рентгеновским данным КТ, принятых в лаборатории. Трехмерной печати томографических данных является мощным визуализации, научно-исследовательских и образовательных инструментов, которые могут теперь получить доступ к доклинической сообщества изображений.
Other articles by Justin M. Diener on PubMed
Imaging and Analysis of Pseudomonas Aeruginosa Swarming and Rhamnolipid Production
Many bacteria spread over surfaces by "swarming" in groups. A problem for scientists who study swarming is the acquisition of statistically significant data that distinguish two observations or detail the temporal patterns and two-dimensional heterogeneities that occur. It is currently difficult to quantify differences between observed swarm phenotypes. Here, we present a method for acquisition of temporal surface motility data using time-lapse fluorescence and bioluminescence imaging. We specifically demonstrate three applications of our technique with the bacterium Pseudomonas aeruginosa. First, we quantify the temporal distribution of P. aeruginosa cells tagged with green fluorescent protein (GFP) and the surfactant rhamnolipid stained with the lipid dye Nile red. Second, we distinguish swarming of P. aeruginosa and Salmonella enterica serovar Typhimurium in a coswarming experiment. Lastly, we quantify differences in swarming and rhamnolipid production of several P. aeruginosa strains. While the best swarming strains produced the most rhamnolipid on surfaces, planktonic culture rhamnolipid production did not correlate with surface growth rhamnolipid production.
Dual Tracer Imaging of SPECT and PET Probes in Living Mice Using a Sequential Protocol
Over the past 20 years, multimodal imaging strategies have motivated the fusion of Positron Emission Tomography (PET) or Single Photon Emission Computed Tomography (SPECT) scans with an X-ray computed tomography (CT) image to provide anatomical information, as well as a framework with which molecular and functional images may be co-registered. Recently, pre-clinical nuclear imaging technology has evolved to capture multiple SPECT or multiple PET tracers to further enhance the information content gathered within an imaging experiment. However, the use of SPECT and PET probes together, in the same animal, has remained a challenge. Here we describe a straightforward method using an integrated trimodal imaging system and a sequential dosing/acquisition protocol to achieve dual tracer imaging with (99m)Tc and (18)F isotopes, along with anatomical CT, on an individual specimen. Dosing and imaging is completed so that minimal animal manipulations are required, full trimodal fusion is conserved, and tracer crosstalk including down-scatter of the PET tracer in SPECT mode is avoided. This technique will enhance the ability of preclinical researchers to detect multiple disease targets and perform functional, molecular, and anatomical imaging on individual specimens to increase the information content gathered within longitudinal in vivo studies.
