Articles by Katherine H. Watkins in JoVE

A Rapid High-throughput Method for Mapping Ribonucleoproteins (RNPs) on Human pre-mRNA

JoVE 1622 12/02/2009

Katherine H. Watkins¹, Allan Stewart¹, William G. Fairbrother^1,2

¹Department of Molecular and Cellular Biology, Brown University, ²Center for Computational Molecular Biology, Brown University

Due to the transient nature of pre-mRNA, it can be difficult to isolate and study in vivo. Here, we present a novel in vitro approach to investigate RNA-protein interactions using a synthetic oligo pool that tiles across selected regions of pre-mRNA.