Articles by Kathryn R. Nightingale in JoVE

Functional Neuroimaging Using Ultrasonic Blood-brain Barrier Disruption and Manganese-enhanced MRI

JoVE 4055 7/12/2012

Gabriel P. Howles¹, Yi Qi², Stephen J. Rosenzweig³, Kathryn R. Nightingale³, G. Allan Johnson²

¹Department of Radiology, Stanford University, ²Center for In Vivo Microscopy, Duke University Medical Center, ³Department of Biomedical Engineering, Duke University

A technique is described for broadly opening the blood-brain barrier in the mouse using microbubbles and ultrasound. Using this technique, manganese can be administered to the mouse brain. Because manganese is an MRI contrast agent that accumulates in depolarized neurons, this approach enables imaging of neuronal activity.