Translate this page to:
In JoVE (1)
Other Publications (1)
Articles by Kishanda Vyboh in JoVE
Detection of Viral RNA by Fluorescence in situ Hybridization (FISH)
Kishanda Vyboh1,2, Lara Ajamian1,3, Andrew J. Mouland1,2,3
1Lady Davis Institute for Medical Research, Sir Mortimer B. Davis Jewish General Hospital, 2Department of Microbiology and Immunology, McGill University, 3Department of Medicine, Division of Experimental Medicine, McGill University
A fluorescence in situ hybridization (FISH) method was developed to visually detect viral genomic RNA using fluorescence microscopy. A probe is made with specificity to the viral RNA that can then be identified using a combination of hybridization and immunofluorescence techniques. This technique offers the advantage of identifying the localization of the viral RNA or DNA at steady-state, providing information on the control of intracellular virus trafficking events.
Other articles by Kishanda Vyboh on PubMed
Human Molecular Genetics. Feb, 2012 | Pubmed ID: 22337953
Spinocerebellar ataxia type 3 (SCA3) is caused by the expansion of the coding CAG repeat in the ATXN3 gene. Interestingly, a -1 base-pair frameshift occurring within an (exp)CAG repeat would henceforth lead to translation from a GCA frame, generating polyalanine stretches instead of polyglutamine. Our results show that transgenic expression of (exp)CAG ATXN3 led to -1 frameshifting events, which are deleterious in Drosophila and mammalian neurons. Conversely, transgenic expression of polyglutamine-encoding (exp)CAA ATXN3 was not toxic. Furthermore, (exp)CAG ATXN3 mRNA does not contribute per se to the toxicity observed in our models. Our observations indicate that expanded polyglutamine tracts in Drosophila and mouse neurons are insufficient for the development of a phenotype. Hence, we propose that -1 ribosomal frameshifting contributes to the toxicity associated with (exp)CAG repeats.