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In JoVE (1)
Other Publications (4)
Articles by Laura Molloy in JoVE
JoVE General
DNA Methylation: Bisulphite Modification and Analysis
1Epigenetics Group, Cancer Research Program, Garvan Institute of Medical Research, 2St Vincent's Clinical School, University of NSW
The gold standard for DNA methylation analysis is genomic sequencing of bisulphite converted DNA. This method takes advantage of the increased sensitivity of cytosine compared with 5-methylcytosine (5-MeC) to bisulphite deamination under acidic conditions. Unmethylated cytosines can be distinguished from methylated cytosines after PCR amplification of the target genomic DNA.
Other articles by Laura Molloy on PubMed
Surgically Induced Astigmatism After Phacoemulsification with and Without Correction for Posture-related Ocular Cyclotorsion: Randomized Controlled Study
To report the impact of posture-related ocular cyclotorsion on one surgeon's surgically induced astigmatism (SIA) results and the variance in SIA.
Periconceptional Undernutrition in Normal and Overweight Ewes Leads to Increased Adrenal Growth and Epigenetic Changes in Adrenal IGF2/H19 Gene in Offspring
Adverse conditions in early life result in increased activation of the hypothalamo-pituitary-adrenal axis and in stress responsiveness in offspring. We have developed a model in which "donor" ewes are either normally nourished or overnourished prior to a period of dietary restriction, before transfer of the embryo at 6-7 d after conception to a ewe of normal weight and nutritional history. A moderate restriction of energy intake during the periconceptional period in both normal weight and overweight ewes resulted in increased adrenal mass in male and female lambs and an increased cortisol response to stress in female lambs. The increase in adrenal weight in lambs exposed to periconceptional undernutrition was associated with a decrease in the adrenal mRNA expression of IGF2 and decreased methylation in the proximal CTCF-binding site in the differentially methylated region of the IGF2/H19 gene. Thus, weight loss in both normal and overweight mothers during the periconceptional period results in epigenetic modification of IGF2 in the adrenal gland, adrenal overgrowth, and increased vulnerability to stress in offspring. Determining the appropriate approach to weight loss in the periconceptional period may therefore be important in overweight or obese women seeking to become pregnant.
Value of Dual Biometry in the Detection and Investigation of Error in the Preoperative Prediction of Refractive Status Following Cataract Surgery
To report the value of dual biometry in the detection of biometry errors.
Estimation of Effective Lens Position Using a Method Independent of Preoperative Keratometry Readings
To evaluate the validity of a keratometry (K)-independent method of estimating effective lens position (ELP) before phacoemulsification cataract surgery.
