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Articles by Lauren Ann Metskas in JoVE
JoVE Clinical and Translational Medicine
Skeletal Muscle Kjønn Dimorphism fra Proteomikk
1Center for Proteomics, Smith College, 2Department of Molecular Biophysics and Biochemistry, Yale University, 3Department of Chemistry, Smith College, 4Department of Biological Sciences and Center for Proteomics, Smith College
En rett-fram sett av metoder for å isolere og fastslå identiteten til de mest tallrike proteinene uttrykt i skjelettmuskulatur. Omtrent 800 flekker er avdekket, på en to-dimensjonal gel fra 10 mg muskler, og dette gjør det mulig for bestemmelse av kjønnsspesifikke protein uttrykk. Disse metodene vil gi tilsvarende resultater i de fleste vev.
Other articles by Lauren Ann Metskas on PubMed
Gender Dimorphism in the Exercise-naïve Murine Skeletal Muscle Proteome
Skeletal muscle is a plastic tissue with known gender dimorphism, especially at the metabolic level. A proteomic comparison of male and female murine biceps brachii was undertaken, resolving an average of 600 protein spots of MW 15-150 kDa and pI 5-8. Twenty-six unique full-length proteins spanning 11 KOG groups demonstrated statistically significant (p<0.05) abundance differences between genders; the majority of these proteins have metabolic functions. Identified glycolytic enzymes demonstrated decreased abundance in females, while abundance differences in identified oxidative phosphorylation enzymes were specific to the proteins rather than to the functional group as a whole. Certain cytoskeletal and stress proteins showed specific expression differences, and all three phosphorylation states of creatine kinase showed significant decreased abundance in females. Expression differences were significant but many were subtle (< or = 2-fold), and known hormonally-regulated proteins were not identified. We conclude that while gender dimorphism is present in non-exercised murine skeletal muscle, the proteome comparison of male and female biceps brachii in exercise-naive mice indicates subtle differences rather than a large or obviously hormonal dimorphism.
