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In JoVE (2)
- Autologe endotheliale voorlopercellen-zaaien Technologie en biocompatibiliteitstests voor cardiovasculaire apparaten in grote Animal Model
- Parallelle plaat flow kamer en Continuous Flow Circuit te endotheliale progenitorcellen Evalueer onder laminaire stroom Afschuifspanning
Other Publications (2)
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Articles by Lauren J. Galinat in JoVE
JoVE Bioengineering
Autologe endotheliale voorlopercellen-zaaien Technologie en biocompatibiliteitstests voor cardiovasculaire apparaten in grote Animal Model
1Department of Biomedical Engineering, Duke University, 2School of Medicine, Duke University, 3Department of Surgery, Duke University Medical Center, 4School of Medicine, University of Pennsylvania
Een methode voor het zaaien titanium bloed-contact biomaterialen met autologe cellen en het testen van biocompatibiliteit wordt beschreven. Deze methode maakt gebruik van endotheliale progenitor cellen en titanium buizen, zonder zaadjes binnen enkele minuten van de chirurgische implantatie in varkens venae cavae. Deze techniek is aan te passen aan vele andere implanteerbare biomedische apparaten.
JoVE Bioengineering
Parallelle plaat flow kamer en Continuous Flow Circuit te endotheliale progenitorcellen Evalueer onder laminaire stroom Afschuifspanning
1Department of Surgery, Duke University Medical Center, 2Department of Biomedical Engineering, Duke University, 3School of Medicine, University of Pennsylvania, 4Department of Medicine, Division of Cardiology, Duke University Medical Center
We beschrijven een methode om onder hechtende cellen aan laminaire stroming shear stress in een steriele continue stroom circuit. De cellen 'hechting, kan morfologie worden bestudeerd door de transparante kamer, monsters verkregen van het circuit voor de metaboliet-analyse en-cellen geoogst na afschuiving blootstelling voor toekomstige experimenten of cultuur.
Other articles by Lauren J. Galinat on PubMed
The Biocompatibility of Titanium Cardiovascular Devices Seeded with Autologous Blood-derived Endothelial Progenitor Cells: EPC-seeded Antithrombotic Ti Implants
Implantable and extracorporeal cardiovascular devices are commonly made from titanium (Ti) (e.g. Ti-coated Nitinol stents and mechanical circulatory assist devices). Endothelializing the blood-contacting Ti surfaces of these devices would provide them with an antithrombogenic coating that mimics the native lining of blood vessels and the heart. We evaluated the viability and adherence of peripheral blood-derived porcine endothelial progenitor cells (EPCs), seeded onto thin Ti layers on glass slides under static conditions and after exposure to fluid shear stresses. EPCs attached and grew to confluence on Ti in serum-free medium, without preadsorption of proteins. After attachment to Ti for 15 min, less than 5% of the cells detached at a shear stress of 100 dyne / cm(2). Confluent monolayers of EPCs on smooth Ti surfaces (Rq of 10 nm), exposed to 15 or 100 dyne/cm(2) for 48 h, aligned and elongated in the direction of flow and produced nitric oxide dependent on the level of shear stress. EPC-coated Ti surfaces had dramatically reduced platelet adhesion when compared to uncoated Ti surfaces. These results indicate that peripheral blood-derived EPCs adhere and function normally on Ti surfaces. Therefore EPCs may be used to seed cardiovascular devices prior to implantation to ameliorate platelet activation and thrombus formation.
Use of Autologous Blood-derived Endothelial Progenitor Cells at Point-of-care to Protect Against Implant Thrombosis in a Large Animal Model
Titanium (Ti) is commonly utilized in many cardiovascular devices, e.g. as a component of Nitinol stents, intra- and extracorporeal mechanical circulatory assist devices, but is associated with the risk of thromboemboli formation. We propose to solve this problem by lining the Ti blood-contacting surfaces with autologous peripheral blood-derived late outgrowth endothelial progenitor cells (EPCs) after having previously demonstrated that these EPCs adhere to and grow on Ti under physiological shear stresses and functionally adapt to their environment under flow conditions ex vivo. Autologous fluorescently-labeled porcine EPCs were seeded at the point-of-care in the operating room onto Ti tubes for 30 min and implanted into the pro-thrombotic environment of the inferior vena cava of swine (n = 8). After 3 days, Ti tubes were explanted, disassembled, and the blood-contacting surface was imaged. A blinded analysis found all 4 cell-seeded implants to be free of clot, whereas 4 controls without EPCs were either entirely occluded or partially thrombosed. Pre-labeled EPCs had spread and were present on all 4 cell-seeded implants while no endothelial cells were observed on control implants. These results suggest that late outgrowth autologous EPCs represent a promising source of lining Ti implants to reduce thrombosis in vivo.
