Articles by Leonard S. Khiroug in JoVE

Gene Delivery to Postnatal Rat Brain by Non-ventricular Plasmid Injection and Electroporation

JoVE 2244 9/17/2010

Dmitry A. Molotkov¹, Alexey Y. Yukin^1,2, Ramil A. Afzalov^1,2, Leonard S. Khiroug¹

¹Neuroscience Center, University of Helsinki, ²Faculty of Biological and Enviromental Sciences, University of Helsinki

This protocol describes a non-viral method of delivery of genetic constructs to a certain area of living rodent brain. The method consists of plasmid preparation, micropipette fabrication, neonatal rat pup surgery, microinjection of the construct, and in vivo electroporation.