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In JoVE (1)
Other Publications (1)
Articles by Liyue Zhang in JoVE
Single Oocyte Bisulfite Mutagenesis
Michelle M. Denomme1,2,3, Liyue Zhang3, Mellissa R.W. Mann1,2,3
1Department of Obstretrics & Gynaecology, Schulich School of Medicine and Dentistry, University of Western Ontario, 2Department of Biochemistry, Schulich School of Medicine and Dentistry, University of Western Ontario, 3Children's Health Research Institute
Bisulfite mutagenesis is the gold standard for analyzing DNA methylation. Our modified protocol allows for DNA methylation analysis at the single-cell level and was specifically designed for individual oocytes. It can also be used for cleavage-stage embryos.
Other articles by Liyue Zhang on PubMed
Journal of Molecular Signaling. 2010 | Pubmed ID: 20594350
CARP-1/CCAR1, a perinuclear phospho-protein, regulates signaling by adriamycin, steroids, or growth factors. However, intracellular events that regulate CARP-1-dependent cell growth are not fully understood.