Articles by Mark D. Bass in JoVE

Induction of Adhesion-dependent Signals Using Low-intensity Ultrasound

JoVE 4024 5/08/2012

James Roper¹, Andrew Harrison², Mark D. Bass¹

¹School of Biochemistry, University of Bristol, ²Smith and Nephew

This protocol describes the stimulation of cultured fibroblasts with low-intensity pulsed ultrasound, which drives focal adhesion formation and Rac1 activation by mimicking engagement of the transmembrane matrix receptor, syndecan-4. This approach allows investigation of a successful clinical technique at the cellular level, thereby providing opportunities for refinement of the therapy.