Articles by Mark Sabbagh in JoVE

Methods for Study of Neuronal Morphogenesis: Ex vivo RNAi Electroporation in Embryonic Murine Cerebral Cortex

JoVE 3621 5/18/2012

Sofia B. Lizarraga^1,2,3, Kathryn R. Coser^1,2, Mark Sabbagh^1,2, Eric M. Morrow^1,2,3

¹Department of Molecular, Cellular Biology and Biochemistry, Brown University, ²Institute for Brain Science, Brown University, ³Department of Psychiatry and Human Behavior, Warren Alpert School of Medicine, Brown University

To conduct a rapid assessment of the function of genes in the development of cerebral cortex, we describe methods involving the ex vivo electroporation of plasmids co-expressing inhibitory RNA (RNAi) and GFP in murine embryonic cortex. This protocol is amenable to the study of various aspects of neurodevelopment such as neurogenesis, neuronal migration and neuronal morphogenesis including dendrite and axon outgrowth.