Articles by Markus Reischl in JoVE

Facilitating Drug Discovery: An Automated High-content Inflammation Assay in Zebrafish

JoVE 4203 7/16/2012

Christine Wittmann¹, Markus Reischl², Asmi H. Shah¹, Ralf Mikut², Urban Liebel^1,2, Clemens Grabher¹

¹Institute for Toxicology and Genetics, Karlsruhe Institute of Technology, Karlsruhe, Germany, ²Institute for Applied Informatics, Karlsruhe Institute of Technology, Karlsruhe, Germany

Here we describe a novel high-content chemically induced inflammation assay aiming at the identification of immune-modulatory bioactives. We have successfully combined automated microscopy with custom developed software scripts enabling automated quantification of the inflammatory response as well as further data processing, analysis, mining, and storage.