Articles by Matthew W. Kay in JoVE

NADH Fluorescence Imaging of Isolated Biventricular Working Rabbit Hearts

JoVE 4115 7/24/2012

Huda Asfour¹, Anastasia M. Wengrowski¹, Rafael Jaimes III¹, Luther M. Swift², Matthew W. Kay¹

¹Electrical and Computer Engineering Department, The George Washington University, ²Pharmacology and Physiology Department, The George Washington University

The objective is to monitor the mitochondrial redox state of isolated hearts within the context of physiologic preload and afterload pressures. A biventricular working rabbit heart model is presented. High spatiotemporal resolution fluorescence imaging of NADH is used to monitor the mitochondrial redox state of epicardial tissue.