Articles by Mehdi Amiri in JoVE

Analysis of Cell Cycle Position in Mammalian Cells

JoVE 3491 1/21/2012

Matthew J. Cecchini¹, Mehdi Amiri¹, Frederick A. Dick²

¹Department of Biochemistry, Schulich School of Medicine and Dentistry, University of Western Ontario, ²London Regional Cancer Program, Children's Health Research Institute, and Department of Biochemistry, Schulich School of Medicine and Dentistry, University of Western Ontario

Determining the cell cycle position of a population of cells, or understanding how signals affect proliferation, can be readily measured by flow cytometry using this protocol. We report a simple experimental approach to staining cells and quantifying their position in the cell cycle.