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In JoVE (1)
Other Publications (1)
Articles by Michael F. Sweeney in JoVE
Microinjection of Zebrafish Embryos to Analyze Gene Function
Jonathan N. Rosen1,2, Michael F. Sweeney1, John D. Mably1,2
1Department of Genetics, Harvard Medical School, 2Department of Cardiology, Children’s Hospital Boston
This video shows how morpholino or mRNA can be injected into zebrafish embryos at the one-cell stage to decrease or increase the level of specific gene products during subsequent development.
Other articles by Michael F. Sweeney on PubMed
Acute Left Atrial Thrombus After Recombinant Factor VIIa Administration During Left Ventricular Assist Device Implantation in a Patient with Heparin-induced Thrombocytopenia
Anesthesia and Analgesia. Feb, 2008 | Pubmed ID: 18227291
We present a patient with end-stage heart failure and heparin-induced thrombocytopenia Type II, who required cardiopulmonary bypass (CPB) during a repeat implantation of a left ventricular assist device for long-term circulatory support. Bivalirudin was selected for anticoagulation during CPB, with concomitant infusion of aprotinin, in an effort to ameliorate blood loss. Nonetheless, profuse bleeding after CPB required massive transfusion of packed red blood cells, multiple coagulation factors, and platelets. Because of persistent bleeding, a single dose of recombinant factor VIIa (rFVIIa, 7.2 mg) was administered as rescue therapy. Within minutes, a large left atrial thrombus was detected by transesophageal echocardiography. We believe this is the first documentation of acute left atrial thrombus formation immediately after a single dose of recombinant factor VIIa administration during a left ventricular assist device implantation.