Articles by Michael J. O'Donovan in JoVE

Retrograde Loading of Nerves, Tracts, and Spinal Roots with Fluorescent Dyes

JoVE 4008 4/19/2012

Dvir Blivis, Michael J. O'Donovan

Developmental Neurobiology Section, National Institute of Neurological Disorders and Stroke, National Institutes of Health

We describe a simple and low cost technique for introducing high concentration of fluorescent and calcium-sensitive dyes into neurons or any neuronal tract using a polyethylene suction pipette.