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In JoVE (1)
Other Publications (28)
- Gynecologic Oncology
- Human Reproduction (Oxford, England)
- Fertility and Sterility
- Fertility and Sterility
- Cancer
- Gynecological Endocrinology : the Official Journal of the International Society of Gynecological Endocrinology
- The Lancet Oncology
- Fertility and Sterility
- Archives of Gynecology and Obstetrics
- Archives of Gynecology and Obstetrics
- Archives of Gynecology and Obstetrics
- Acta Obstetricia Et Gynecologica Scandinavica
- Archives of Gynecology and Obstetrics
- Archives of Gynecology and Obstetrics
- Reproductive Biomedicine Online
- Proceedings of the National Academy of Sciences of the United States of America
- Prenatal Diagnosis
- Archives of Gynecology and Obstetrics
- Archives of Gynecology and Obstetrics
- Journal of Reproductive Immunology
- Archives of Gynecology and Obstetrics
- Archives of Gynecology and Obstetrics
- Archives of Gynecology and Obstetrics
- Archives of Gynecology and Obstetrics
- Archives of Gynecology and Obstetrics
- Archives of Gynecology and Obstetrics
- Archives of Gynecology and Obstetrics
- Archives of Gynecology and Obstetrics
Articles by Michael K. Bohlmann in JoVE
JoVE Clinical and Translational Medicine
A Human Fallopian Tube Model for Investigation of C. trachomatis Infections
1Institute of Medical Microbiology and Hygiene, University of Lübeck, 2Institute of Anatomie, University of Lübeck, 3Department of Obstetrics and Gynecology, University Hospital of Schleswig-Holstein, University of Lübeck, 4Medical Clinic III, University Hospital of Schleswig-Holstein, University of Lübeck
We describe an ex vivo infection model for visualisation of direct interactions from bacterial pathogens with human fallopian tube cells. The whole organ tissue model was established to investigate C. trachomatis induced pathology to the female fallopian tube under "life-like" conditions.
Other articles by Michael K. Bohlmann on PubMed
Intracardiac Resection of a Metastatic Choriocarcinoma
Postpartal choriocarcinoma is a rare complication of pregnancy, which usually responds well to chemotherapy, even in advanced stages. Metastatic heart involvement typically characterizes a widely advanced disease, requiring palliative treatment.
Osteopontin is Up-regulated in Human Decidual Stromal Cells
To analyze expression and regulation of osteopontin in human stromal endometrial and decidual cells.
Glucocorticoid Administration in Antiemetic Therapy: is It Safe?
Although glucocorticoids are often used in cancer therapy, in particular to enhance the effectiveness of antiemetic therapy, they have been associated with impaired tumor apoptosis and an increased frequency of metastases in some reports. The current study aimed to determine whether glucocorticoid treatment had an adverse effect on outcomes in patients with ovarian carcinoma.
Intraoperative Venous Blood Sampling to Localize a Small Androgen-producing Ovarian Tumor
Among other causes of virilization, ovarian tumors can be the cause of excessive androgen production. We report the case of a Leydig cell tumor of the ovary where diagnostic attempts to localize the source of hyperandrogenism preoperatively failed owing to relatively small tumor size.
Squamous-cell Carcinoma in Mature Cystic Teratoma of the Ovary: Systematic Review and Analysis of Published Data
Up to a quarter of ovarian masses originate from germ cells, and many of these are mature cystic teratomas. The secondary development of malignancy is a rare but well-known phenomenon in patients with ovarian teratomas. Squamous-cell carcinoma accounts for 80% of secondary malignant transformations of ovarian teratomas. We aimed to do an up-to-date systematic review of this rare malignant transformation. 64 suitable studies provided information on 277 patients. Squamous-cell carcinoma in mature cystic teratoma was mainly found in women aged more than 50 years, with high concentrations of squamous-cell-carcinoma antigen and cancer antigen CA125, and with ovarian tumours more than 100 mm in size. Patients with FIGO stage Ia tumours had better survival than those with more advanced disease. Complete resection together with hysterectomy, bilateral salpingo-oophorectomy and lymphadenectomy for patients with advanced disease, followed by adjuvant chemotherapy with an alkylating drug was associated with higher survival, radiotherapy was not. We make proposals for investigation and treatment of this rare disorder.
Isolated Pyosalpinx in a 13-year-old Virgin
To describe the successful laparoscopic management of an isolated pyosalpinx in a 13-year-old virgin.
Advanced Age is a Risk Factor for Higher Grade Perineal Lacerations During Delivery in Nulliparous Women
To identify risk factors for the development of severe perineal lacerations and to give recommendations for their prevention in nulliparous women.
Nulliparous Patients with Small Stature Delivering at Term Have an Increased Risk of Secondary Cesarean Section
To evaluate the obstetric outcome of pregnant patients with small stature (<5th percentile) with regard to the mode of delivery, maternal injuries, and neonatal parameters.
Specific Secretory Phase Endometrial Leukocytes of Women with Two and More Consecutive Idiopathic Abortions Are Not Significantly Different from Healthy Controls
To analyze concentrations of endometrial leukocytes in patients with idiopathic-repeated abortions.
Seven Years' Experience in Laparoscopic Dissection of Intact Ovarian Dermoid Cysts
A large case series on laparoscopic removal of dermoid cysts with a diameter between 3 and 12 cm, via an endobag, is reported (127 cysts in 121 premenopausal women). The incidence of spillage and recurrence rate of laparoscopic ovarian dermoid cystectomy, the duration of the surgical procedure, the length of hospitalization, the incidence of recurrence and pregnancies was evaluated. In 2.5% of cases, the endobag ruptured during removal, and a total spillage rate of 12% was seen. No signs or symptoms of peritonitis were observed regardless of cystic spillage or not. Laparoscopic cystectomy of dermoid cysts in premenopausal women is safe and effective and appears to be a valuable alternative to laparotomy. Controlled intraperitoneal spillage of cyst contents does not increase postoperative morbidity as long as an endobag is used and the peritoneal cavity is washed out thoroughly.
GnRH Analogs Do Not Protect Ovaries from Chemotherapy-induced Ultrastructural Injury in Hodgkin's Lymphoma Patients
To determine the protective effect of gonadotropin-releasing hormone analogs (GnRHa) using several ultrasound and endocrine markers to detect ultrastructural ovarian damage in Hodgkin's lymphoma patients.
Hysteroscopic Findings in Women with Two and with More Than Two First-trimester Miscarriages Are Not Significantly Different
The purpose of this study was to analyse hysteroscopic results in patients with recurrent miscarriages and to compare the frequency of uterine anomalies in women with a history of exactly two and with more than two consecutive miscarriages. A retrospective analysis of 206 patients undergoing hysteroscopy for repeated early pregnancy losses was performed at two university centres. Late miscarriages were excluded, terminations of pregnancy were not counted. Eighty-seven patients had suffered from exactly two early miscarriages and 119 from more than two. Both groups were comparable with respect to age at admission (32.95+/-4.46 versus 34.06+/-5.02 years) and at first miscarriage (30.43+/-4.24 versus 29.08+/-5.38 years). The prevalence of acquired (adhesions, polyps, fibroids) and congenital uterine anomalies (septate or bicornuate uterus, etc.) did not differ significantly (acquired: 28.7 versus 27.7%; congenital: 9.2 versus 16.8%). The rates of uterine anomalies did not differ significantly overall (36.8 versus 42.9%). In conclusion, uterine anomalies are frequently found in patients with two and with more than two early miscarriages. Due to the high rate of anomalies, their risk for adverse pregnancy outcome and a possible therapeutic approach, hysteroscopy might be a diagnostic option even after two early miscarriages.
Hypoxia Abrogates Antichlamydial Properties of IFN-γ in Human Fallopian Tube Cells in Vitro and Ex Vivo
IFN-γ has an important role in the adaptive immune response against intracellular pathogens. In urogenital tract (UGT) infections with the obligate intracellular pathogen Chlamydia trachomatis, IFN-γ-mediated control of chlamydial growth implies the JAK-STAT signaling cascades and subsequent induction of the indoleamine 2,3-dioxygenase (IDO). As oxygen concentrations in the UGT are low under physiological conditions (O(2) < 5%) and further decrease during an inflammatory process, we wondered whether antibacterial properties of IFN-γ are maintained under hypoxic conditions. Using primary cells that were isolated from human fallopian tubes and an ex vivo human fallopian tube model (HFTM), we found that even high IFN-γ concentrations (200 units/mL) were not sufficient to limit growth of C. trachomatis under hypoxia. Reduced antibacterial activity of IFN-γ under hypoxia was restricted to the urogenital serovars D and L(2), but was not observed with the ocular serovar A. Impaired effectiveness of IFN-γ on chlamydial growth under hypoxia was accompanied by reduced phosphorylation of Stat-1 on Tyr701 and diminished IDO activity. This study shows that IFN-γ effector functions on intracellular C. trachomatis depend on the environmental oxygen supply, which could explain inadequate bacterial clearance and subsequent chronic infections eventually occurring in the UGT of women.
Fetal Micrognathia: Objective Assessment and Associated Anomalies on Prenatal Sonogram
To determine the accuracy and characteristics of prenatally detected fetal micrognathia.
Role of Fetal Abdominal Circumference As a Prognostic Parameter of Perinatal Complications
To evaluate the potential of fetal abdominal circumference (AC) measurement as predictor of perinatal complications in term newborns.
Current Diagnostic Modalities and Clinical Pitfalls in Malignant Secondary Breast Tumours
Breast cancer is the most common malignancy in women with increasing incidence. The occurrence of metastatic disease to the breast in both females and males is relatively rare, constituting 0.5-6% of all breast malignancies. The therapy of secondary breast cancer is usually completely different from that of primary malignant lesions. Thus, early confirmation of secondary involvement is crucial to direct appropriate and to avoid potentially harmful treatment. The main focus remains to include secondary breast involvement in the differential diagnosis of breast tumours. In recent years, a number of new or improved imaging modalities have been introduced in the diagnosis of breast neoplasm. Current diagnostic concepts of metastatic neoplasm to the breast, including latest imaging modalities and their possible future applications, are presented in this review with special emphasis on possible pitfalls.
Effects and Effectiveness of Heparin in Assisted Reproduction
Despite significant improvements in treatment protocols and laboratory techniques, the baby take-home rate after assisted reproduction (ART) is disappointing, e.g. 20% in Germany. It is therefore understandable that various approaches are undertaken to improve the pregnancy and delivery rates after IVF and ICSI. Among others, the application of heparin is suggested to avoid implantation failure - partially irrespective of maternal thrombophilia. The applications of heparin for various indications are presented in this manuscript and critically discussed, taking into account current studies and meta-analyses. Furthermore, possible effects of heparin on the embryo-maternal dialogue and indications for heparin to prevent and treat thromboembolic disease during ART are summarized. A systematic literature search of the PubMed/Medline database was performed assessing all articles published before November 2010. "IVF", "ICSI", "assisted reproduction", "heparin", "side effect", "coagulation", "thrombosis", "thrombophilia", "thrombophilic disorder", "implantation", "implantation failure" and "placenta" were used as keywords. The available studies on heparin in assisted reproduction are characterized by heterogeneous inclusion criteria and a lack of proven effectiveness in special constellations. In conclusion, the application of heparin to improve ART outcome rates is not justified.
Spontaneous Uterine Rupture at the 21st Week of Gestation Caused by Placenta Percreta
To describe the management of a ruptured uterus caused by placenta percreta in the 21st week of gestation.
Cancer in Pregnancy. Part II: Treatment Options of Breast and Other Non-gynecological Malignancies
In case of non-gynecological solid tumors and hematological malignancies diagnosed during pregnancy, individual diagnostic and treatment options must be established by an interdisciplinary team.
Does Acetic Acid Influence the Non-dysplastic Pap Smear?
Acetic acid tests are commonly performed for colposcopic evaluation of the cervix. However, it is unclear whether the acetic acid influences normal Papanicolaou (Pap) smear results.
Standardized Technique of Laparoscopic Pelvic and Para-aortic Lymphadenectomy in Gynecologic Cancer Optimizes the Perioperative Outcomes
The main objective of this study is to illustrate the effectiveness and the safety of standardized technique of laparoscopic lymphadenectomy (LNE), newly introduced in a University Hospital, in patients with gynecologic malignancy.
Successful Application of Vacuum-assisted Closure Therapy for Treatment of Mastitis-associated Chronic Breast Wounds
Although non-puerperal mastitis is rare and its cause is rather unclear, the number of patients diagnosed with this defect is increasing. In some cases, standard therapy fails and it progresses to a chronic disease. Vacuum-assisted closure (VAC) therapy has shown good results in healing complex wounds.
Heparanase Expression in Term Placentas of Diabetic Patients and Healthy Controls
The prevalence of diabetic disorders in pregnancy is rising, which goes along with increased risks for maternal and foetal complications during pregnancy and delivery. The expression of the endo-β-D: -glucuronidase, heparanase (HPSE), may increase under hyperglycaemic conditions, is believed to play an important role in diabetes associated morbidity outside the female reproductive tract and is expressed in the placenta throughout gestation. However, the placental expression of HPSE has not been investigated in diabetic patients.
Cancer in Pregnancy. Part I: Basic Diagnostic and Therapeutic Principles and Treatment of Gynecological Malignancies
Cancer in pregnancy is a rare circumstance. However, the coincidence of pregnancy and malignancy is supposed to increase due to a general tendency of postponing childbearing to older age. To date, clinical guidelines are scarce and experience regarding therapeutic management is limited to case reports.
Primary Metastasized Extraskeletal Ewing Sarcoma of the Vulva: Report of a Case and Review of the Literature
Ewing's sarcoma (ES) and primitive neuroectodermal tumor (PNET) are closely related malignant neoplasms, usually affecting the skeletal system. Extraosseous ES/PNETs are uncommon, with occasional reports of tumors affecting the genitourinary tract. Only few cases of primary vulvar Ewing's sarcoma/PNET have previously been reported.
