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JoVE Neuroscience Isolation and Culture of Hippocampal Neurons from Prenatal Mice JoVE 3634 7/26/2012 Michael L. Seibenhener, Marie W. Wooten Department of Biological Sciences, Auburn University We provide a protocol for the culture of highly purified hippocampal neurons from prenatal mouse brains without the use of a feeder glial cell layer.