Articles by Michael V. Johnston in JoVE

Derivation of Glial Restricted Precursors from E13 mice

JoVE 3462 6/20/2012

André W. Phillips^1,2, Sina Falahati^1,2, Roshi DeSilva^1,3, Irina Shats², Joel Marx¹, Edwin Arauz¹, Douglas A. Kerr⁴, Jeffrey D. Rothstein^2,5, Michael V. Johnston^1,2,6, Ali Fatemi^1,2,6

¹Hugo W. Moser Research Institute at Kennedy Krieger, Johns Hopkins University, ²Department of Neurology, Johns Hopkins School of Medicine, ³University of Maryland, ⁴Experimental Neurology, Biogen Idec, ⁵The Brain Science Institute, Johns Hopkins School of Medicine, ⁶Department of Pediatrics, Johns Hopkins School of Medicine

This protocol outlines the derivation of Glial Restricted Precursors from fetal spinal cords and maintained in vitro either for transplantation or for the study of oligodendrocytic lineage.