Articles by Michelle M. Denomme in JoVE

Single Oocyte Bisulfite Mutagenesis

JoVE 4046 6/27/2012

Michelle M. Denomme^1,2,3, Liyue Zhang³, Mellissa R.W. Mann^1,2,3

¹Department of Obstretrics & Gynaecology, Schulich School of Medicine and Dentistry, University of Western Ontario, ²Department of Biochemistry, Schulich School of Medicine and Dentistry, University of Western Ontario, ³Children's Health Research Institute

Bisulfite mutagenesis is the gold standard for analyzing DNA methylation. Our modified protocol allows for DNA methylation analysis at the single-cell level and was specifically designed for individual oocytes. It can also be used for cleavage-stage embryos.