Articles by Orrin J. Stone in JoVE

Automated Hydrophobic Interaction Chromatography Column Selection for Use in Protein Purification

JoVE 3060 9/21/2011

Patrick J. M. Murphy¹, Orrin J. Stone², Michelle E. Anderson¹

¹College of Nursing, Interdisciplinary Life Sciences Research Laboratory, Seattle University, ²College of Science and Engineering, Interdisciplinary Life Sciences Research Laboratory, Seattle University

An automated method for identifying suitable hydrophobic interaction chromatography (HIC) media to be used in the process of protein purification is presented. The method utilizes a medium-pressure liquid chromatography system including automated buffer blending, dynamic sample loop injection, sequential column selection, multi-wavelength analysis, and split fraction eluate collection.