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In JoVE (1)
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Articles by Pablo R. Helguera in JoVE
JoVE Neuroscience
Cryopreservation of Cortical Tissue Blocks for the Generation of Highly Enriched Neuronal Cultures
Department of Neurobiology and Behavior, University of California, Irvine
Here, we describe a method for efficient cryopreservation and thawing of cortical brain tissue blocks to generate highly enriched neuronal cultures. This simple protocol provides flexibility for later generation of neuronal, astrocyte, and neuronal precursor cell cultures.
