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JoVE General Quantitative Measurement of GLUT4 Translocation to the Plasma Membrane by Flow Cytometry JoVE 2429 11/07/2010 Shyny Koshy, Parema Alizadeh, Lubov T. Timchenko, Christine Beeton Department of Molecular Physiology and Biophysics, Baylor College of Medicine This protocol describes a rapid technique to quantify the translocation of GLUT4 from the cytoplasm to the plasma membrane of cells by flow cytometry.