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In JoVE (1)
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Articles by Paule Cousineau-Pelletier in JoVE
Preparação de tendões da cauda de rato de Estudos biomecânicos e Mechanobiological
Amélie Bruneau, Nadia Champagne, Paule Cousineau-Pelletier, Gabriel Parent, Eve Langelier
Groupe PERSEUS, Faculté de Génie Département de génie mécanique, Université de Sherbrooke
Este artigo descreve os procedimentos experimentais usados para preparar os tendões da cauda de rato para estudos biomecânicos e mechanobiological. Várias características dos principais passos na preparação são demonstrados, começando com a extração, transversal medição da área, lavagem e carregamento na câmara biorreator.
Other articles by Paule Cousineau-Pelletier on PubMed
Relative Contributions of Mechanical Degradation, Enzymatic Degradation, and Repair of the Extracellular Matrix on the Response of Tendons when Subjected to Under- and Over- Mechanical Stimulations in Vitro
Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society. Feb, 2010 | Pubmed ID: 19725106
Tendon response to mechanical loading results in either homeostasis, improvement, or degeneration of tissue condition. In an effort to better understand the development of tendinopathies, this study investigated the mechanical and structural responses of tendons subjected to under- and over-stimulations (1.2% and 1.8% strain respectively, 1 Hz). The objective was to examine three sub-processes of tendon response: mechanical degradation, enzymatic degradation, and repair of the extracellular matrix. We subjected rat tail tendons to a 10-day stimulation protocol with four periods of 6 h each day: 30 min of stimulation and 5 h 30 min of rest. To investigate the contribution of the three sub-processes, we controlled the contribution of the cells through variations in the nutrient and protease inhibitor content in the in vitro solutions. Using nondestructive cyclic tests, we evaluated the daily changes in the peak stress. To assess structural changes, we carried out microscopic analyses at the end of the study period. We observed that the relative contributions of the sub-processes differed according to the stimulation amplitude. With over-stimulation of tendons immersed in DMEM, we succeeded in reducing enzymatic degradation and increasing peak stress. In under-stimulation, the addition of protease inhibitors was required to obtain the same result.