Peter Hegemann Experimental Biophysics, Institute of Biology Humboldt-Universität zu Berlin Biography Publications Institution JoVE Articles Peter Hegemann has not added a biography. If you are Peter Hegemann and would like to personalize this page please email our Author Liaison for assistance. Publications Complex Photochemistry Within the Green-Absorbing Channelrhodopsin ReaChR Biophysical Journal. Mar, 2017 | Pubmed ID: 28355544 An Efficient Visual Screen for CRISPR/Cas9 Activity in Arabidopsis Thaliana Frontiers in Plant Science. 2017 | Pubmed ID: 28174584 Active Cortical Dendrites Modulate Perception Science (New York, N.Y.). Dec, 2016 | Pubmed ID: 28008068 Photoadduct Formation from the FMN Singlet Excited State in the LOV2 Domain of Chlamydomonas Reinhardtii Phototropin The Journal of Physical Chemistry Letters. Nov, 2016 | Pubmed ID: 27766868 A Blue-light Photoreceptor Mediates the Feedback Regulation of Photosynthesis Nature. 09, 2016 | Pubmed ID: 27626383 Unfolding of the C-Terminal Jα Helix in the LOV2 Photoreceptor Domain Observed by Time-Resolved Vibrational Spectroscopy The Journal of Physical Chemistry Letters. Sep, 2016 | Pubmed ID: 27537211 Optogenetic Approaches Addressing Extracellular Modulation of Neural Excitability Scientific Reports. Apr, 2016 | Pubmed ID: 27045897 Identification of a Natural Green Light Absorbing Chloride Conducting Channelrhodopsin from Proteomonas Sulcata The Journal of Biological Chemistry. Feb, 2016 | Pubmed ID: 26740624 Structural Foundations of Optogenetics: Determinants of Channelrhodopsin Ion Selectivity Proceedings of the National Academy of Sciences of the United States of America. Jan, 2016 | Pubmed ID: 26699459 Light-Induced Rearrangement of the β5 Strand in the BLUF Photoreceptor SyPixD (Slr1694) The Journal of Physical Chemistry Letters. Dec, 2015 | Pubmed ID: 26631358 Optogenetic Acidification of Synaptic Vesicles and Lysosomes Nature Neuroscience. Dec, 2015 | Pubmed ID: 26551543 An Improved Chloride-conducting Channelrhodopsin for Light-induced Inhibition of Neuronal Activity in Vivo Scientific Reports. 10, 2015 | Pubmed ID: 26443033 Optogenetics: 10 Years After ChR2 in Neurons--views from the Community Nature Neuroscience. Sep, 2015 | Pubmed ID: 26308981 Photoactivated Cyclases: In Memoriam Masakatsu Watanabe Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology. Oct, 2015 | Pubmed ID: 26270352 The Rhodopsin-guanylyl Cyclase of the Aquatic Fungus Blastocladiella Emersonii Enables Fast Optical Control of CGMP Signaling Science Signaling. Aug, 2015 | Pubmed ID: 26268609 Light-Dark Adaptation of Channelrhodopsin Involves Photoconversion Between the All-trans and 13-cis Retinal Isomers Biochemistry. Sep, 2015 | Pubmed ID: 26237332 Time-resolved Infrared Spectroscopic Techniques As Applied to Channelrhodopsin Frontiers in Molecular Biosciences. 2015 | Pubmed ID: 26217670 Biophysics of Channelrhodopsin Annual Review of Biophysics. 2015 | Pubmed ID: 26098512 Photochemical Chromophore Isomerization in Histidine Kinase Rhodopsin HKR1 FEBS Letters. Apr, 2015 | Pubmed ID: 25836735 Controlling Fertilization and CAMP Signaling in Sperm by Optogenetics ELife. Jan, 2015 | Pubmed ID: 25601414 Early Formation of the Ion-conducting Pore in Channelrhodopsin-2 Angewandte Chemie (International Ed. in English). Apr, 2015 | Pubmed ID: 25537168 Upgrading a Microplate Reader for Photobiology and All-optical Experiments Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology. Feb, 2015 | Pubmed ID: 25373866 Absorption and Emission Spectroscopic Characterization of Photo-dynamics of Photoactivated Adenylyl Cyclase Mutant BPAC-Y7F of Beggiatoa Sp Journal of Photochemistry and Photobiology. B, Biology. Nov, 2014 | Pubmed ID: 25154810 Key Residues for the Light Regulation of the Blue Light-activated Adenylyl Cyclase from Beggiatoa Sp Biochemistry. Aug, 2014 | Pubmed ID: 25046330 Engineering of a Red-light-activated Human CAMP/cGMP-specific Phosphodiesterase Proceedings of the National Academy of Sciences of the United States of America. Jun, 2014 | Pubmed ID: 24889611 Conversion of Channelrhodopsin into a Light-gated Chloride Channel Science (New York, N.Y.). Apr, 2014 | Pubmed ID: 24674867 Bistable Retinal Schiff Base Photodynamics of Histidine Kinase Rhodopsin HKR1 from Chlamydomonas Reinhardtii Photochemistry and Photobiology. Jul-Aug, 2014 | Pubmed ID: 24460585 Microbial and Animal Rhodopsins: Structures, Functions, and Molecular Mechanisms Chemical Reviews. Jan, 2014 | Pubmed ID: 24364740 Of Ion Pumps, Sensors and Channels - Perspectives on Microbial Rhodopsins Between Science and History Biochimica Et Biophysica Acta. May, 2014 | Pubmed ID: 23994288 A Set of Engineered Escherichia Coli Expression Strains for Selective Isotope and Reactivity Labeling of Amino Acid Side Chains and Flavin Cofactors PloS One. 2013 | Pubmed ID: 24223875 Gloeobacter Rhodopsin, Limitation of Proton Pumping at High Electrochemical Load Biophysical Journal. Nov, 2013 | Pubmed ID: 24209850 Biotechnology: Programming Genomes with Light Nature. Aug, 2013 | Pubmed ID: 23969454 Ion Selectivity and Competition in Channelrhodopsins Biophysical Journal. Jul, 2013 | Pubmed ID: 23823227 Optogenetic Modulation of an Adenylate Cyclase in Toxoplasma Gondii Demonstrates a Requirement of the Parasite CAMP for Host-cell Invasion and Stage Differentiation The Journal of Biological Chemistry. May, 2013 | Pubmed ID: 23525100 Adventure Beyond Borders of Scientific Fields with Optogenetics Neuroscience Research. Jan, 2013 | Pubmed ID: 23465316 Genetically Encoded Calcium Indicators for Multi-color Neural Activity Imaging and Combination with Optogenetics Frontiers in Molecular Neuroscience. 2013 | Pubmed ID: 23459413 Light-dark Adaptation of Channelrhodopsin C128T Mutant The Journal of Biological Chemistry. Apr, 2013 | Pubmed ID: 23439646 Towards an Understanding of Channelrhodopsin Function: Simulations Lead to Novel Insights of the Channel Mechanism Journal of Molecular Biology. May, 2013 | Pubmed ID: 23376098 Genome of Acanthamoeba Castellanii Highlights Extensive Lateral Gene Transfer and Early Evolution of Tyrosine Kinase Signaling Genome Biology. Feb, 2013 | Pubmed ID: 23375108 From Channelrhodopsins to Optogenetics EMBO Molecular Medicine. Feb, 2013 | Pubmed ID: 23339069 Nuclear Gene Targeting in Chlamydomonas Using Engineered Zinc-finger Nucleases The Plant Journal : for Cell and Molecular Biology. Mar, 2013 | Pubmed ID: 23137232 Phototropin Influence on Eyespot Development and Regulation of Phototactic Behavior in Chlamydomonas Reinhardtii The Plant Cell. Nov, 2012 | Pubmed ID: 23204408 Bimodal Activation of Different Neuron Classes with the Spectrally Red-shifted Channelrhodopsin Chimera C1V1 in Caenorhabditis Elegans PloS One. 2012 | Pubmed ID: 23056472 A Photochromic Histidine Kinase Rhodopsin (HKR1) That is Bimodally Switched by Ultraviolet and Blue Light The Journal of Biological Chemistry. Nov, 2012 | Pubmed ID: 23027869 Color-tuned Channelrhodopsins for Multiwavelength Optogenetics The Journal of Biological Chemistry. Sep, 2012 | Pubmed ID: 22843694 Crystal Structure of the Channelrhodopsin Light-gated Cation Channel Nature. Jan, 2012 | Pubmed ID: 22266941 Structural Model of Channelrhodopsin The Journal of Biological Chemistry. Mar, 2012 | Pubmed ID: 22241469 In Channelrhodopsin-2 Glu-90 is Crucial for Ion Selectivity and is Deprotonated During the Photocycle The Journal of Biological Chemistry. Feb, 2012 | Pubmed ID: 22219197 The Microbial Opsin Family of Optogenetic Tools Cell. Dec, 2011 | Pubmed ID: 22196724 The Chromophore Structure of the Long-lived Intermediate of the C128T Channelrhodopsin-2 Variant FEBS Letters. Dec, 2011 | Pubmed ID: 22094167 Color Tuning in Binding Pocket Models of the Chlamydomonas-type Channelrhodopsins The Journal of Physical Chemistry. B. Dec, 2011 | Pubmed ID: 22077286 Rectification of the Channelrhodopsin Early Conductance Biophysical Journal. Sep, 2011 | Pubmed ID: 21889442 Neocortical Excitation/inhibition Balance in Information Processing and Social Dysfunction Nature. Sep, 2011 | Pubmed ID: 21796121 The Hydrogen-bond Switch Reaction of the Blrb Bluf Domain of Rhodobacter Sphaeroides The Journal of Physical Chemistry. B. Jun, 2011 | Pubmed ID: 21627064 Introduction to the Symposium-in Print: Blue Light Effects Photochemistry and Photobiology. May-Jun, 2011 | Pubmed ID: 21517885 High-efficiency Channelrhodopsins for Fast Neuronal Stimulation at Low Light Levels Proceedings of the National Academy of Sciences of the United States of America. May, 2011 | Pubmed ID: 21504945 Microbial Opsins: a Family of Single-component Tools for Optical Control of Neural Activity Cold Spring Harbor Protocols. Mar, 2011 | Pubmed ID: 21363959 Light-generated Paramagnetic Intermediates in BLUF Domains Photochemistry and Photobiology. May-Jun, 2011 | Pubmed ID: 21198648 Channelrhodopsin Engineering and Exploration of New Optogenetic Tools Nature Methods. Jan, 2011 | Pubmed ID: 21191371 Light Modulation of Cellular CAMP by a Small Bacterial Photoactivated Adenylyl Cyclase, BPAC, of the Soil Bacterium Beggiatoa The Journal of Biological Chemistry. Jan, 2011 | Pubmed ID: 21030594 Fast, Repetitive Light-activation of CaV3.2 Using Channelrhodopsin 2 Channels (Austin, Tex.). May-Jun, 2010 | Pubmed ID: 20714225 Hindered Rotation of a Cofactor Methyl Group As a Probe for Protein-cofactor Interaction Journal of the American Chemical Society. Jul, 2010 | Pubmed ID: 20536240 Evolution of the Channelrhodopsin Photocycle Model Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry. Apr, 2010 | Pubmed ID: 20349494 The Branched Photocycle of the Slow-cycling Channelrhodopsin-2 Mutant C128T Journal of Molecular Biology. May, 2010 | Pubmed ID: 20346954 Two Open States with Progressive Proton Selectivities in the Branched Channelrhodopsin-2 Photocycle Biophysical Journal. Mar, 2010 | Pubmed ID: 20197028 Ultrafast Optogenetic Control Nature Neuroscience. Mar, 2010 | Pubmed ID: 20081849 Blue Light Induces Global and Localized Conformational Changes in the Kinase Domain of Full-length Phototropin Biochemistry. Feb, 2010 | Pubmed ID: 20052995 The Role of Key Amino Acids in the Photoactivation Pathway of the Synechocystis Slr1694 BLUF Domain Biochemistry. Dec, 2009 | Pubmed ID: 19863128 Mechanistic Insights in Light-induced CAMP Production by Photoactivated Adenylyl Cyclase Alpha (PACalpha) Biological Chemistry. Nov, 2009 | Pubmed ID: 19747080 Channelrhodopsins of Volvox Carteri Are Photochromic Proteins That Are Specifically Expressed in Somatic Cells Under Control of Light, Temperature, and the Sex Inducer Plant Physiology. Sep, 2009 | Pubmed ID: 19641026 Glu 87 of Channelrhodopsin-1 Causes PH-dependent Color Tuning and Fast Photocurrent Inactivation Photochemistry and Photobiology. Mar-Apr, 2009 | Pubmed ID: 19192197 Nuclear Gene Targeting in Chlamydomonas As Exemplified by Disruption of the PHOT Gene Gene. Mar, 2009 | Pubmed ID: 19121376 Bi-stable Neural State Switches Nature Neuroscience. Feb, 2009 | Pubmed ID: 19079251 In Vivo Generation of Flavoproteins with Modified Cofactors Journal of Molecular Biology. Feb, 2009 | Pubmed ID: 19027027 Monitoring Light-induced Structural Changes of Channelrhodopsin-2 by UV-visible and Fourier Transform Infrared Spectroscopy The Journal of Biological Chemistry. Dec, 2008 | Pubmed ID: 18927082 Hydrogen Bond Switching Among Flavin and Amino Acid Side Chains in the BLUF Photoreceptor Observed by Ultrafast Infrared Spectroscopy Biophysical Journal. Nov, 2008 | Pubmed ID: 18708458 Dynamics of Voltage Profile in Enzymatic Ion Transporters, Demonstrated in Electrokinetics of Proton Pumping Rhodopsin Biophysical Journal. Dec, 2008 | Pubmed ID: 18621842 Channelrhodopsin-1 Initiates Phototaxis and Photophobic Responses in Chlamydomonas by Immediate Light-induced Depolarization The Plant Cell. Jun, 2008 | Pubmed ID: 18552201 Algal Sensory Photoreceptors Annual Review of Plant Biology. 2008 | Pubmed ID: 18444900 Red-shifted Optogenetic Excitation: a Tool for Fast Neural Control Derived from Volvox Carteri Nature Neuroscience. Jun, 2008 | Pubmed ID: 18432196 Photoactivation of Channelrhodopsin The Journal of Biological Chemistry. Jan, 2008 | Pubmed ID: 17993465 Redox Properties of LOV Domains: Chemical Versus Photochemical Reduction, and Influence on the Photocycle Chembiochem : a European Journal of Chemical Biology. Dec, 2007 | Pubmed ID: 17990262 Fast Manipulation of Cellular CAMP Level by Light in Vivo Nature Methods. Jan, 2007 | Pubmed ID: 17128267 Hydrogen-bond Switching Through a Radical Pair Mechanism in a Flavin-binding Photoreceptor Proceedings of the National Academy of Sciences of the United States of America. Jul, 2006 | Pubmed ID: 16829579 H+ -pumping Rhodopsin from the Marine Alga Acetabularia Biophysical Journal. Aug, 2006 | Pubmed ID: 16731558 The Photochemistry of the Light-, Oxygen-, and Voltage-sensitive Domains in the Algal Blue Light Receptor Phot Biopolymers. Jul, 2006 | Pubmed ID: 16552739 Fast Noninvasive Activation and Inhibition of Neural and Network Activity by Vertebrate Rhodopsin and Green Algae Channelrhodopsin Proceedings of the National Academy of Sciences of the United States of America. Dec, 2005 | Pubmed ID: 16306259 Multiple Photocycles of Channelrhodopsin Biophysical Journal. Dec, 2005 | Pubmed ID: 16169986 Nuclear-gene Targeting by Using Single-stranded DNA Avoids Illegitimate DNA Integration in Chlamydomonas Reinhardtii Eukaryotic Cell. Jul, 2005 | Pubmed ID: 16002652 The Phot LOV2 Domain and Its Interaction with LOV1 Biophysical Journal. Jul, 2005 | Pubmed ID: 15879473 Removal of Mismatched Bases from Synthetic Genes by Enzymatic Mismatch Cleavage Nucleic Acids Research. Mar, 2005 | Pubmed ID: 15800209 Monitoring Dynamic Expression of Nuclear Genes in Chlamydomonas Reinhardtii by Using a Synthetic Luciferase Reporter Gene Plant Molecular Biology. Aug, 2004 | Pubmed ID: 15604722 On the Reaction Mechanism of Adduct Formation in LOV Domains of the Plant Blue-light Receptor Phototropin Journal of the American Chemical Society. Sep, 2004 | Pubmed ID: 15339193 "Vision" in Single-celled Algae News in Physiological Sciences : an International Journal of Physiology Produced Jointly by the International Union of Physiological Sciences and the American Physiological Society. Jun, 2004 | Pubmed ID: 15143209 Recording of Blue Light-induced Energy and Volume Changes Within the Wild-type and Mutated Phot-LOV1 Domain from Chlamydomonas Reinhardtii Biophysical Journal. Feb, 2004 | Pubmed ID: 14747340 Channelrhodopsin-2, a Directly Light-gated Cation-selective Membrane Channel Proceedings of the National Academy of Sciences of the United States of America. Nov, 2003 | Pubmed ID: 14615590 Irreversible Photoreduction of Flavin in a Mutated Phot-LOV1 Domain Biochemistry. Aug, 2003 | Pubmed ID: 12924934 Characterization of a Flavin Radical Product in a C57M Mutant of a LOV1 Domain by Electron Paramagnetic Resonance Biochemistry. Jul, 2003 | Pubmed ID: 12859197 Crystal Structures and Molecular Mechanism of a Light-induced Signaling Switch: The Phot-LOV1 Domain from Chlamydomonas Reinhardtii Biophysical Journal. Apr, 2003 | Pubmed ID: 12668455 Phot-LOV1: Photocycle of a Blue-light Receptor Domain from the Green Alga Chlamydomonas Reinhardtii Biophysical Journal. Feb, 2003 | Pubmed ID: 12547798 Channelrhodopsin-1: a Light-gated Proton Channel in Green Algae Science (New York, N.Y.). Jun, 2002 | Pubmed ID: 12089443 Evidence for a Light-induced H(+) Conductance in the Eye of the Green Alga Chlamydomonas Reinhardtii Biophysical Journal. Feb, 2002 | Pubmed ID: 11806916 Whole-cell Patch-clamp Recordings for Electrophysiological Determination of Ion Selectivity in Channelrhodopsins Christiane Grimm*1, Johannes Vierock*1, Peter Hegemann1, Jonas Wietek1 1Experimental Biophysics, Institute of Biology, Humboldt-Universität zu Berlin JoVE 55497 Neuroscience
Whole-cell Patch-clamp Recordings for Electrophysiological Determination of Ion Selectivity in Channelrhodopsins Christiane Grimm*1, Johannes Vierock*1, Peter Hegemann1, Jonas Wietek1 1Experimental Biophysics, Institute of Biology, Humboldt-Universität zu Berlin JoVE 55497 Neuroscience
