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Articles by Praechompoo Pongtornpipat in JoVE

 JoVE Clinical and Translational Medicine

High Throughput Sequential ELISA for Validation of Biomarkers of Acute Graft-Versus-Host Disease

1Pediatric Blood and Marrow Transplant Program, University of Michigan


JoVE 4247

High throughput validation of multiple candidate biomarkers can be performed by sequential ELISA in order to minimize freeze/thaw cycles and use of precious plasma samples. Here, we demonstrate how to sequentially perform ELISAs for six different validated plasma biomarkers1-3 of graft-versus-host disease (GVHD)4 on the same plasma sample.

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