The Influence of Youth Music Television Viewership on Changes in Cigarette Use and Association with Smoking Peers: A Social Identity, Reinforcing Spirals Perspective

Communication Research. Dec, 2010  |  Pubmed ID: 21318085

Prior research has found strong evidence of a prospective association between R movie exposure and teen smoking. Using parallel process latent-growth modeling, the present study examines prospective associations between viewing of music video channels on television (e.g., MTV and VH-1) and changes over time in smoking and association with smoking peers. Results showed that baseline viewing of music-oriented channels such as MTV and VH-1 robustly predicted increasing trajectories of smoking and of associating with smoking peers, even after application of a variety of controls including parent reports of monitoring behavior. These results are consistent with the arguments from the reinforcing spirals model that such media use serves as a means of developing emergent adolescent social identities consistent with associating with smoking peers and acquiring smoking and other risk behaviors; evidence also suggests that media choice in reinforcing spiral processes are dynamic and evolve as social identity evolves.

Safety of Neonatal Cleft Lip Repair

Journal of Plastic Surgery and Hand Surgery. Nov, 2010  |  Pubmed ID: 21446820

The timing of repair of a cleft lip continues to be debated. One of the reasons for delaying operation has been the belief that there is an increased risk of anaesthesia during the neonatal period. As a contribution to this debate we have analysed the anaesthetic and surgical complications of neonatal cleft lip repair undertaken at a single centre. We made a retrospective study of 99 babies consecutively referred for repair of cleft lip over a five-year period (January 1995-December 1999). In contrast to other series, all babies were considered for neonatal surgery and no exclusion criteria were set. All repairs were undertaken within 28 days of birth (median 4); the median gestational age was 40 weeks (range 34-42) and median birth weight of 3300 g (range 1500-4600 g). Perianaesthetic complications included one case of hypoxia presumably as a result of transitional circulation, one reintubation for poor respiratory effort in a premature baby, and five cases of nasal obstruction, three of which required a nasal stent. All recovered without long-term effects. There were significantly more surgical complications with bilateral repairs than with unilateral (p < 0.03). Breast feeding was achieved in 54 babies by the time of discharge. We found no evidence that neonatal repair of cleft lip is unsafe. Paediatric anaesthetic and intensive care support within a specialised centre are necessary, and close postoperative monitoring is required, with attention to the nasal airway.

The Shaping of the Face Space in Early Infancy: Becoming a Native Face Processor

Child Development Perspectives. Dec, 2010  |  Pubmed ID: 21562620

Face perception remains one of the most intensively researched areas in psychology and allied disciplines, and there has been much debate regarding the early origins and experiential determinants of face processing. This article reviews studies, the majority of which have appeared in the past decade, that discuss possible mechanisms underlying face perception at birth and document the prominent role of experience in shaping infants' face-processing abilities. In the first months of life, infants develop a preference for female and own-race faces and become better able to recognize and categorize own-race and own-species faces. This perceptual narrowing and shaping of the "face space" forms a foundation for later face expertise in childhood and adulthood and testifies to the remarkable plasticity of the developing visual system.

Surface Energy and Surface Proton Order of the Ice Ih Basal and Prism Surfaces

Journal of Physics. Condensed Matter : an Institute of Physics Journal. Feb, 2010  |  Pubmed ID: 21386387

Density-functional theory (DFT) is used to examine the basal and prism surfaces of ice Ih. Similar surface energies are obtained for the two surfaces; however, in each case a strong dependence of the surface energy on surface proton order is identified. This dependence, which can be as much as 50% of the absolute surface energy, is significantly larger than the bulk dependence (<1%) on proton order, suggesting that the thermodynamic ground state of the ice surface will remain proton ordered well above the bulk order-disorder temperature of about 72 K. On the basal surface this suggestion is supported by Monte Carlo simulations with an empirical potential and solution of a 2D Ising model with nearest neighbor interactions taken from DFT. Order parameters that define the surface energy of each surface in terms of nearest neighbor interactions between dangling OH bonds (those which point out of the surface into vacuum) have been identified and are discussed. Overall, these results suggest that proton order-disorder effects have a profound impact on the stability of ice surfaces and will most likely have an effect on ice surface reactivity as well as ice crystal growth and morphology.

Administering Labelled Water to Exclusively Breast-fed Infants in Studies Involving Stable Isotope Dilution Techniques

Isotopes in Environmental and Health Studies. Mar, 2011  |  Pubmed ID: 21390987

Stable isotope techniques using deuterium or (18)O are reference methods for assessing total body water (TBW) for body composition. In combination, they provide total energy expenditure and human milk intake in exclusively breast-fed (EBF) infants. These techniques require an oral administration of an accurately quantified dose of labelled water to infants, who often have no prior experience of consuming water. In the present study, (18)O labelled water was administered to 47 EBF infants at two time points. Route of administration, duration of dosing and spills were quantified and recorded. Eighty-seven out of 94 (92.6%) dose administrations were successful. In two-thirds of dose administrations, >90% of the prepared dose was consumed. Spills ranged from 0.2% to 57%. Approaches to correct for spills can introduce bias in the calculation of TBW. Minimising and recording all dose spills is an important issue for the accuracy and precision of stable isotope techniques, when applied to EBF infants.

An Animal Model for Measuring the Effect of Common NICU Procedures on ATP Metabolism

Biological Research for Nursing. Jul, 2011  |  Pubmed ID: 21406502

Neonates exposed to common neonatal intensive care unit (NICU) procedures show alterations in heart rate, blood pressure, and oxygen saturation. However, it is unclear if these physiologic changes increase adenosine triphosphate (ATP) utilization, thus potentially increasing the risk for tissue hypoxia in medically fragile neonates. Plasma uric acid is a commonly used marker of increased ATP utilization because uric acid levels increase when ATP consumption is enhanced. To examine the effect of a common NICU procedure on plasma uric acid concentration, we developed a model that allows for acute monitoring of this biochemical marker in plasma in 7- to 9-day-old rabbits. In our pilot study, we exposed neonatal rabbits to a single heel lance 2.5 hr after catheter placement. We measured uric acid concentration before and 30 min after the heel lance and compared findings to levels in control animals not exposed to the heel lance. Our pilot data shows a significant difference in uric acid concentration over time between the control and heel lance groups (46.2 ± 7.1 μM vs. 54.7 ± 5.8 μM, respectively, p = .027). Calculation of percentage change from baseline showed uric acid concentration increasing in rabbits exposed to heel lance and decreasing in control rabbits (1.5 ± 4.7% vs. -16.1 ± 4.2%, respectively, p = .03). These data suggest that this animal model can be successfully used to examine the biochemical effect of common NICU procedures, such as heel lance, on markers of ATP breakdown and purine metabolism.

EHR: a Match Made in Heaven

MGMA Connexion / Medical Group Management Association. Feb, 2011  |  Pubmed ID: 21409871

PH-responsive Polymers for Trehalose Loading and Desiccation Protection of Human Red Blood Cells

Biomaterials. Jul, 2011  |  Pubmed ID: 21421265

PP-50, a synthetic pH-responsive biopolymer, is here shown to increase the permeability of the phospholipid bilayer to trehalose, a disaccharide accumulated in desiccation tolerant organisms across all kingdoms. Uptake of 251 ± 6 mm intracellular trehalose facilitated an increase in the membrane integrity of vacuum dried cells by a factor of 9 ± 1 and reduced extent of hemoglobin oxidation in dried cells from 66 ± 1% to 23 ± 3%. To elucidate the mechanism of PP-50 mediated trehalose delivery, permeability studies were conducted using molecules ranging in size from sucrose to 10 kDa poly(ethylene glycol). It was shown that the logarithm of relative diffusant membrane permeability decreased linearly with diffusant molecular volume, suggesting transport via non-Stokesian diffusion. Consistent with this conclusion, topographic atomic force micrographs reported membrane thinning proximate to PP-50 adsorption on the erythrocyte membrane, a phenomenon associated with increased incidence of phospholipid hydrocarbon chain bending.

Utility of Combined Two-dimensional and Three-dimensional Transesophageal Imaging for Catheter-based Mitral Valve Clip Repair of Mitral Regurgitation

Journal of the American Society of Echocardiography : Official Publication of the American Society of Echocardiography. Jun, 2011  |  Pubmed ID: 21435839

Catheter-based mitral valve clip repair (CBMCR) is feasible for selected patients with mitral regurgitation (MR). Two-dimensional (2D) transesophageal echocardiography (TEE) is the standard modality for evaluating MR and procedural guidance. Recently, real-time three-dimensional TEE became available. The aim of this study was to evaluate the value of combined 2D and three-dimensional TEE for CBMCR. In evaluating MR for CBMCR, the confidence of interpretation of 2D TEE was compared with that of combined imaging for the localization of major valve pathology. In patients who underwent CBMCR, the outcomes and the duration of CBMCR were compared.

Relationships Between Energy Balance Knowledge and the Home Environment

Journal of the American Dietetic Association. Apr, 2011  |  Pubmed ID: 21443988

Certain aspects of the home environment as well as individuals' knowledge of energy balance are believed to be important correlates of various dietary and physical activity behaviors, but no known studies have examined potential relationships between these correlates. This study evaluated cross-sectional associations between characteristics of the home environment and energy balance knowledge among 349 youth/parent pairs recruited from the Minneapolis/St Paul, MN, metropolitan area from September 2006 to June 2007. Linear regression models adjusted for student grade and highest level of parental education were used to compare data from home food, physical activity, and media inventories (parent-reported) with energy balance knowledge scores from youth and parent questionnaires. Paired energy balance knowledge (average of youth and parent knowledge scores) was associated with all home food availability variables. Paired knowledge was also significantly associated with a media equipment availability and accessibility summary score (β=-1.40, P=0.005), as well as an activity-to-media ratio score (β=0.72, P=0.003). Youth and/or parent knowledge alone was not significantly associated with most characteristics of the home environment, supporting the importance of developing intervention strategies that target the family as a whole.

Effects of Optimal Medical Treatment with or Without Coronary Revascularization on Angina and Subsequent Revascularizations in Patients with Type 2 Diabetes Mellitus and Stable Ischemic Heart Disease

Circulation. Apr, 2011  |  Pubmed ID: 21444887

In the Bypass Angioplasty Revascularization Investigation 2 Diabetes (BARI 2D) trial, an initial strategy of coronary revascularization and optimal medical treatment (REV) compared with an initial optimal medical treatment with the option of subsequent revascularization (MED) did not reduce all-cause mortality or the composite of cardiovascular death, myocardial infarction, and stroke in patients with type 2 diabetes mellitus and stable ischemic heart disease. In the same population, we tested whether the REV strategy was superior to the MED strategy in preventing worsening and new angina and subsequent coronary revascularizations.

White Matter Changes in Basis Pontis in Small Expansion FMR1 Allele Carriers with Parkinsonism

American Journal of Medical Genetics. Part B, Neuropsychiatric Genetics : the Official Publication of the International Society of Psychiatric Genetics. Jun, 2011  |  Pubmed ID: 21445959

Examples of white matter hyperintensities (wmh) on magnetic resonance images in a basis pontis are presented in two male carriers, each of whom carry a small CGG expansion fragile X mental retardation (FMR1) allele. One carried a premutation (PM) allele of 85 CGG repeats and the other, a gray zone (GZ) allele of 47 repeats. Both were originally diagnosed with idiopathic Parkinson's disease (iPD). Similar changes are also shown in one PM carrier of 99 repeats affected with mild tremor and imbalance, who was ascertained through a fragile X syndrome family. These examples draw attention to the occurrence of wmh in a basis pontis in the carriers of small CGG expansions presenting with tremor and ataxia. Moreover, the presence of this change in GZ, as well as PM, allele carriers originally diagnosed with iPD supports our earlier suggestion that both these alleles may contribute to the neurodegenerative changes in this disorder which, in the examples presented, have been reflected by wmh, most prominent in the cerebellar peduncles and/or pontine area.

The Discovery of Benzanilides As C-Met Receptor Tyrosine Kinase Inhibitors by a Directed Screening Approach

Bioorganic & Medicinal Chemistry Letters. Sep, 2011  |  Pubmed ID: 21835616

A directed screen of a relatively small number of compounds, selected for kinase ATP pocket binding potential, yielded a novel series of hit compounds (1). Hit explosion on two binding residues identified compounds 27 and 43 as the best leads for an optimization program having reduced secondary metabolism, as measured by in vitro rat hepatocytes incubation, leading to oral bio-availability. Structure-activity relationships and molecular modeling have suggested a binding mode for the most potent inhibitor 12.

Prevention of Postpartum Smoking Relapse in Mothers of Infants in the Neonatal Intensive Care Unit

Journal of Perinatology : Official Journal of the California Perinatal Association. Aug, 2011  |  Pubmed ID: 21836549

Objective:Approximately 40% of women who smoke tobacco quit smoking during pregnancy, yet up to 85% relapse after delivery. Those who resume smoking often do so by 2 to 8 weeks postpartum. Smoking mothers are more than twice as likely to quit breastfeeding by 10 weeks postpartum. The hospitalization of a newborn, while stressful, is an opportunity to emphasize the importance of a smoke-free environment for babies. Supporting maternal-infant bonding may reduce maternal stress and motivate mothers to remain smoke free and continue breastfeeding. The objective of this study was to reduce postpartum smoking relapse and prolong breastfeeding duration during the first 8 weeks postpartum in mothers who quit smoking just before or during pregnancy and have newborns admitted to the Neonatal Intensive Care Unit (NICU).Study Design:This study was an Institutional Review Board-approved prospective randomized clinical trial. After informed consent, mothers of newborns admitted to the NICU were randomized to a control or intervention group. Both groups received weekly encouragement to remain smoke free and routine breastfeeding support. Mothers in the intervention group were also given enhanced support for maternal-infant bonding including information about newborn behaviors, and were encouraged to frequently hold their babies skin-to-skin.Result:More mothers were smoke free (81 vs 46%, P<0.001) and breastfeeding (86 vs 21%, P<0.001) in the intervention than in the control group at 8 weeks postpartum.Conclusion:Interventions to support mother-infant bonding during a newborn's hospitalization in the NICU are associated with reduced rates of smoking relapse and prolonged duration of breastfeeding during the first 8 weeks postpartum.Journal of Perinatology advance online publication, 11 August 2011; doi:10.1038/jp.2011.106.

Very Low Risk of Thromboembolic Events in Patients Undergoing Successful Catheter Ablation of Atrial Fibrillation with a CHADS2 Score ‰�3: a Long-term Outcome Study

Circulation. Arrhythmia and Electrophysiology. Oct, 2011  |  Pubmed ID: 21841192

Long-term cessation of oral anticoagulation (OAC) after catheter ablation of atrial fibrillation (AF) has been deemed controversial. The safety of this management strategy in patients without recurrent AF and with historically elevated risks for thromboembolism remains largely unknown. In this study, we sought to evaluate the long-term results of OAC cessation after successful catheter ablation of AF.

Understanding the Social Networks of Parents of Children with Sickle Cell Disease

Journal of Health Care for the Poor and Underserved. Aug, 2011  |  Pubmed ID: 21841293

Although there is substantial literature documenting the challenges of pediatric sickle cell disease (SCD) for children and their parents, there is limited research identifying how parents prioritize their needs and use their social networks to manage information regarding their child's SCD in terms of physical and mental health. We examined parents' perceived needs regarding child health issues as they relate to SCD; who and what sources of information are utilized by parents regarding SCD; the frequency with which they consult these resources; and the level at which they trust them. Parents in this study reported that mothers, physicians, the Internet, and books were key sources of support, guidance, and counsel regarding the health needs of children with SCD. These three sources were rated high in importance, trust, frequency of contact, and perceived supportiveness toward mental and physical health needs.

Why Addressing the Poor and Underinsured is Vexing

Health Communication. Sep, 2011  |  Pubmed ID: 21843099

Polymer Dewetting Via Stimuli Responsive Structural Relaxation-contact Angle Analysis

Chemical Communications (Cambridge, England). Oct, 2011  |  Pubmed ID: 21847489

Thin films of a stimuli-responsive homopolymer dewet as a stimulus response after anion exchange of the imidazolium's counter anion. Contact angle analysis and interfacial energy considerations indicate dewetting goes counter to increasing spreading coefficient. Intrafilm stress arising from structural relaxation drives the dewetting.

Extending the Scope of Diagnostic Chromosome Analysis: Detection of Single Gene Defects Using High-resolution SNP Microarrays

Human Mutation. Dec, 2011  |  Pubmed ID: 21850686

Microarray analysis has provided significant advances in the diagnosis of conditions resulting from submicroscopic chromosome abnormalities. It has been recommended that array testing should be a "first tier" test in the evaluation of individuals with intellectual disability, developmental delay, congenital anomalies, and autism. The availability of arrays with increasingly high probe coverage and resolution has increased the detection of decreasingly small copy number changes (CNCs) down to the intragenic or even exon level. Importantly, arrays that genotype SNPs also detect extended regions of homozygosity. We describe 14 examples of single gene disorders caused by intragenic changes from a consecutive set of 6,500 tests using high-resolution SNP microarrays. These cases illustrate the increased scope of cytogenetic testing beyond dominant chromosome rearrangements that typically contain many genes. Nine of the cases confirmed the clinical diagnosis, that is, followed a "phenotype to genotype" approach. Five were diagnosed by the laboratory analysis in the absence of a specific clinical diagnosis, that is, followed a "genotype to phenotype" approach. Two were clinically significant, incidental findings. The importance of astute clinical assessment and laboratory-clinician consultation is emphasized to optimize the value of microarrays in the diagnosis of disorders caused by single gene copy number and sequence mutations.

Expression of Genes Involved in Mouse Lung Cell Differentiation/regulation After Acute Exposure to Photons and Protons with or Without Low-dose Preirradiation

Radiation Research. Nov, 2011  |  Pubmed ID: 21854213

The goal of this study was to compare the effects of acute 2 Gy irradiation with photons (0.8 Gy/min) or protons (0.9 Gy/min), both with and without pre-exposure to low-dose/low-dose-rate γ rays (0.01 Gy at 0.03 cGy/h), on 84 genes involved in stem cell differentiation or regulation in mouse lungs on days 21 and 56. Genes with a ≥1.5-fold difference in expression and P < 0.05 compared to 0 Gy controls are emphasized. Two proteins specific for lung stem cells/progenitors responsible for local tissue repair were also compared. Overall, striking differences were present between protons and photons in modulating the genes. More genes were affected by protons than by photons (22 compared to 2 and 6 compared to 2 on day 21 and day 56, respectively) compared to 0 Gy. Preirradiation with low-dose-rate γ rays enhanced the acute photon-induced gene modulation on day 21 (11 compared to 2), and all 11 genes were significantly downregulated on day 56. On day 21, seven genes (aldh2, bmp2, cdc2a, col1a1, dll1, foxa2 and notch1) were upregulated in response to most of the radiation regimens. Immunoreactivity of Clara cell secretory protein was enhanced by all radiation regimens. The number of alveolar type 2 cells positive for prosurfactant protein C in irradiated groups was higher on day 56 (12.4-14.6 cells/100) than on day 21 (8.5-11.2 cells/100) (P < 0.05). Taken together, these results showed that acute photons and protons induced different gene expression profiles in the lungs and that pre-exposure to low-dose-rate γ rays sometimes had modulatory effects. In addition, proteins associated with lung-specific stem cells/progenitors were highly sensitive to radiation.

Assessing Compliance with National Comprehensive Cancer Network Guidelines for Elderly Patients with Stage III Colon Cancer: the Fox Chase Cancer Center Partners' Initiative

Clinical Colorectal Cancer. Jun, 2011  |  Pubmed ID: 21859563

Fox Chase Cancer Center Partners (FCCCP) performs an annual quality review of affiliate practices based on National Comprehensive Cancer Network (NCCN) guidelines. Given recent treatment advances, we initiated this medical record review in elderly patients with stage III colon cancer to measure compliance with these guidelines.

Safety of Ablation for Atrial Fibrillation with Therapeutic INR: Comparison with Transition to Low-molecular-weight Heparin

Arquivos Brasileiros De Cardiologia. Oct, 2011  |  Pubmed ID: 21861038

The ideal management of oral anticoagulation (OAC) before and after catheter ablation for atrial fibrillation (AF) is still controversial.

Measurement of the W+ W- Cross Section in Sqrt(s) = 7  TeV Pp Collisions with ATLAS

Physical Review Letters. Jul, 2011  |  Pubmed ID: 21866993

This Letter presents a measurement of the W+ W- production cross section in sqrt(s) = 7  TeV pp collisions by the ATLAS experiment, using 34  pb(-1) of integrated luminosity produced by the Large Hadron Collider at CERN. Selecting events with two isolated leptons, each either an electron or a muon, 8 candidate events are observed with an expected background of 1.7 ± 0.6 events. The measured cross section is 41(-16)(+20)(stat) ± 5(syst)±1(lumi)  pb, which is consistent with the standard model prediction of 44 ± 3  pb calculated at next-to-leading order in QCD.

Proximal Thoracic Aortic Replacement for Aneurysmal Disease Using the Freestyle Stentless Bioprosthesis: a 10-year Experience

The Annals of Thoracic Surgery. Sep, 2011  |  Pubmed ID: 21871272

Porcine bioprosthesis (bioroots) are an attractive surgical strategy for ascending aorta and arch replacement. This study evaluated the perioperative and late outcomes using this strategy for proximal aortic aneurysmal disease.

CRH Acts on CRH-R1 and -R2 to Differentially Modulate the Expression of Large-conductance Calcium-activated Potassium Channels in Human Pregnant Myometrium

Endocrinology. Nov, 2011  |  Pubmed ID: 21878514

CRH has been implicated to play a key role in the control of human pregnancy and parturition. Large-conductance potassium channels (BKCa) play a pivotal role in the modulation of uterine contractility during pregnancy. The objectives of the present study were to investigate the effect of CRH on BKCa expression in human pregnant myometrial cells. Myometrial tissues were collected at cesarean section from pregnant women not-in-labor (TNL) or in-labor (TL) at term, and myocytes were isolated and cultured. CRH was identified in human pregnant myometrium and mainly expressed in myometrial myocytes. Cultured myometrial cells were able to secrete CRH. In TNL myometrial cells, CRH treatment increased the expression of BKCa α- and β-subunits. CRH receptor type 1 (CRH-R1) antagonist, antalarmin, decreased whereas CRH receptor type 2 (CRH-R2) antagonist, astressin2b, increased the expression of BKCa. CRH-R2 small interfering RNA (siRNA) caused an increase, but CRH-R1 siRNA resulted in a decrease, in BKCa expression. In contrast to TNL cells, CRH exhibited an opposite effect on BKCa expression in TL myometrial cells, i.e. decreased BKCa expression. Antalarmin enhanced but astressin2b reduced BKCa expression. CRH-R2 siRNA decreased whereas CRH-R1 siRNA increased BKCa expression. 1,3-Dihydro-1-[2-hydroxy-5-(trifluoromethyl)phenyl]-5-(trifluoromethyl)-2H-benzimidazol-2-one significantly inhibited the frequency of spontaneous contractions of myometrial strips, and this effect was significantly decreased in TL strips compared with TNL ones. Our data suggest that CRH-R1 and CRH-R2 show differential regulation of BKCa expression. These effects mediated by CRH-R1 and CRH-R2 are changed after the onset of labor. This leads us to suggest that CRH may fine-tune myometrial contractility by modulating the expression of BKCa during pregnancy and labor.

Communications During Epidural Catheter Placement for Labour Analgesia

Anaesthesia. Nov, 2011  |  Pubmed ID: 21883126

Evidence suggests that anaesthetists' communication can affect patient experience. There is a lack of guidance for anaesthetists as to the optimal verbal communication to use during insertion of epidurals on the labour ward. We recorded the verbal communication used by 14 anaesthetists during the siting of epidural catheters in women on the labour ward; a classification of the language used was subsequently devised. We found that commands and information statements were the most common types of communication used. Individual anaesthetists differed markedly in their use of positive and negative verbal language. This classification of verbal communication that we produced may be of value in future training and research of verbal communication used by anaesthetists on the labour ward.

Referral of Diabetic Macular Oedema by Australian Optometrists: Comment

Clinical & Experimental Ophthalmology. Dec, 2011  |  Pubmed ID: 21883778

Large Variation of Vacancy Formation Energies in the Surface of Crystalline Ice

Nature Materials. Oct, 2011  |  Pubmed ID: 21892176

Resolving the atomic structure of the surface of ice particles within clouds, over the temperature range encountered in the atmosphere and relevant to understanding heterogeneous catalysis on ice, remains an experimental challenge. By using first-principles calculations, we show that the surface of crystalline ice exhibits a remarkable variance in vacancy formation energies, akin to an amorphous material. We find vacancy formation energies as low as ~0.1-0.2 eV, which leads to a higher than expected vacancy concentration. Because a vacancy's reactivity correlates with its formation energy, ice particles may be more reactive than previously thought. We also show that vacancies significantly reduce the formation energy of neighbouring vacancies, thus facilitating pitting and contributing to pre-melting and quasi-liquid layer formation. These surface properties arise from proton disorder and the relaxation of geometric constraints, which suggests that other frustrated materials may possess unusual surface characteristics.

Parent- and Child-reported Parenting. Associations with Child Weight-related Outcomes

Appetite. Dec, 2011  |  Pubmed ID: 21896297

The present study aimed to investigate associations of both parent-reported and child-perceived parenting styles and parent-reported parenting practices with child weight and weight-related behaviours. Participants were 175 children (56% female) aged between 7 and 11, and their primary caregivers (91% female), recruited through South Australian primary schools. Children completed measures of parenting style, attitude toward fruit, vegetables, and non-core food, and attraction to physical activity. Parents completed measures of parenting style and domain-specific parenting practices (feeding and activity-related practices) and reported on child dietary intake, physical activity, and sedentary behaviour. Objective height and weight measurements were taken from children, from which body mass index (BMI) was calculated. Child-reported parenting style and parent-reported parenting practices were uniquely associated with child weight-related outcomes, but styles and practices did not interact in their association with child outcomes. Child-reported parenting style was associated with child food and activity attitudes, whereas parent-reported parenting style was not associated with child outcomes. The findings of the present study generally support the recommendation of a parenting style high in demandingness and responsiveness for supporting healthy child weight-related behaviours, along with appropriate domain-specific practices. The child's perspective should be incorporated into research involving child outcomes wherever possible.

A Retrospective Study of the Value of Indirect CT Venography: a British Perspective

The British Journal of Radiology. Sep, 2011  |  Pubmed ID: 21896661

Objectives: The aim of this study was to establish the value of indirect CT venography (CTV) in a clinical practice within the UK. Methods: 804 combined CT pulmonary angiogram (CTPA) and CTV studies were retrospectively reviewed. CTV was performed 180 s following the injection of contrast using an incremental technique with a 5 mm collimation and a 5 cm interspace between images extending from the iliac crests to the tibial plateaus. Results: 12.9% of studies had isolated pulmonary emboli (PE), 3.0% had both a PE and deep vein thrombosis (DVT) and 1.1% had an isolated DVT. The proportion of positive cases diagnosed by CTV alone was 6.6%. Conclusion: In a UK based practice the incidence and the proportion of isolated DVT diagnosed by CTV are lower than expected from published data. An analysis of possible causes for this is made within the paper.

Rewards Can Be Used Effectively with Repeated Exposure to Increase Liking of Vegetables in 4-6-year-old Children

Public Health Nutrition. Sep, 2011  |  Pubmed ID: 21899792

OBJECTIVE: To examine whether parents offering a sticker reward to their child to taste a vegetable the child does not currently consume is associated with improvements in children's liking and consumption of the vegetable. DESIGN: A randomized controlled trial evaluated the effectiveness of exposure only (EO) and exposure plus reward (E + R), relative to a control group, on children's liking and consumption of a target vegetable. Assessments were conducted at baseline and 2 weeks from baseline (post-intervention). Follow-up assessments were conducted at 4 weeks and 3 months from baseline. SETTING: The study took place in Adelaide, South Australia. Participants were self-selected in response to local media advertisements seeking to recruit parents finding it difficult to get their children to eat vegetables. SUBJECTS: Participants were 185 children (110 boys, seventy-five girls) aged 4-6 years and their primary caregiver/parent (172 mothers, thirteen fathers). RESULTS: The E + R group was able to achieve more days of taste exposure. Both EO and E + R increased liking at post-intervention compared with control and no further change occurred over the follow-up period. All groups increased their intake of the target vegetable at post-intervention. Target vegetable consumption continued to increase significantly over the follow-up period for E + R and control but not for EO. CONCLUSIONS: The findings provide support for the effectiveness of using a sticker reward with a repeated exposure strategy. In particular, such rewards can facilitate the actual tastings necessary to change liking.

Mechanisms of Myocardial Infarction in Women Without Angiographically Obstructive Coronary Artery Disease

Circulation. Sep, 2011  |  Pubmed ID: 21900087

There is no angiographically demonstrable obstructive coronary artery disease (CAD) in a significant minority of patients with myocardial infarction, particularly women. We sought to determine the mechanism(s) of myocardial infarction in this setting using multiple imaging techniques.

A Shift in Sensory Processing That Enables the Developing Human Brain to Discriminate Touch from Pain

Current Biology : CB. Sep, 2011  |  Pubmed ID: 21906948

When and how infants begin to discriminate noxious from innocuous stimuli is a fundamental question in neuroscience [1]. However, little is known about the development of the necessary cortical somatosensory functional prerequisites in the intact human brain. Recent studies of developing brain networks have emphasized the importance of transient spontaneous and evoked neuronal bursting activity in the formation of functional circuits [2, 3]. These neuronal bursts are present during development and precede the onset of sensory functions [4, 5]. Their disappearance and the emergence of more adult-like activity are therefore thought to signal the maturation of functional brain circuitry [2, 4]. Here we show the changing patterns of neuronal activity that underlie the onset of nociception and touch discrimination in the preterm infant. We have conducted noninvasive electroencephalogram (EEG) recording of the brain neuronal activity in response to time-locked touches and clinically essential noxious lances of the heel in infants aged 28-45 weeks gestation. We show a transition in brain response following tactile and noxious stimulation from nonspecific, evenly dispersed neuronal bursts to modality-specific, localized, evoked potentials. The results suggest that specific neural circuits necessary for discrimination between touch and nociception emerge from 35-37 weeks gestation in the human brain.

Post-mortem Findings in 54 Cases of Anesthetic Associated Death in Cats from Two Spay-neuter Programs in New York State

Journal of Feline Medicine and Surgery. Dec, 2011  |  Pubmed ID: 21907603

Anesthetic-associated death (AAD) in cats is infrequent, but occurs far more frequently than in people. Post-mortem investigations of AAD in cats are uncommon, and results only sporadically published. Here we report the findings in 54 cases of AAD in cats. Significant gross and/or microscopic pre-existing disease, including pulmonary, cardiac, and systemic disease, was detected in 33% of cases. Pulmonary disease was most frequently diagnosed (24% of cases), and included cases of Aelurostrongylus abstrusus infection (9% of cases). Heart disease, including two cases of hypertrophic cardiomyopathy, was less frequent (11% of cases). Four percent died from surgical complications. No significant gross or microscopic disease was detected in 63% of cases. Additional studies are needed to determine if these findings are representative of AAD in cats in other geographic areas or with access to veterinary care. This study demonstrates that post-mortem investigation of AADs is an important and worthwhile endeavor.

A Critical Appraisal of Endorectal Ultrasound and Transanal Endoscopic Microsurgery and Decision-making in Early Rectal Cancer

Colorectal Disease : the Official Journal of the Association of Coloproctology of Great Britain and Ireland. Sep, 2011  |  Pubmed ID: 21920011

Aim:  Transanal endoscopic microsurgery (TEM) for early rectal cancer (ERC) gives similar results to major surgery in selected cases. Endorectal ultrasound (ERUS) is an important part of the preoperative selection process. This study reports its accuracy and impact for patients entered on the UK TEM database. Method:  The UK TEM database comprises prospectively collected data on 494 patients. This dataset was used to determine the prevalence of ERUS in pre-operative staging and its accuracy by comparing pre-operative T-stage with definitive pathological staging following TEM. Results:  ERUS was performed in 165 of the 494 patients who underwent TEM for rectal cancer. It inaccurately staged rectal cancer in 44.8% of cases including 32.7% of tumours understaged and 12.1% overstaged. There was no significant difference in the depth of TEM excision or R1 rate between the patients who underwent ERUS prior to TEM and those who did not (p value=0.73). Conclusion:  The data show that ERUS is employed in a minority of rectal cancers undergoing TEM in the UK and its accuracy in this 'Real World' practice is disappointing.

Mouse Genomic Variation and Its Effect on Phenotypes and Gene Regulation

Nature. Sep, 2011  |  Pubmed ID: 21921910

We report genome sequences of 17 inbred strains of laboratory mice and identify almost ten times more variants than previously known. We use these genomes to explore the phylogenetic history of the laboratory mouse and to examine the functional consequences of allele-specific variation on transcript abundance, revealing that at least 12% of transcripts show a significant tissue-specific expression bias. By identifying candidate functional variants at 718 quantitative trait loci we show that the molecular nature of functional variants and their position relative to genes vary according to the effect size of the locus. These sequences provide a starting point for a new era in the functional analysis of a key model organism.

Large-scale Transcriptional Profiling and Functional Assays Reveal Important Roles for Rho-GTPase Signalling and SCL During Haematopoietic Differentiation of Human Embryonic Stem Cells

Human Molecular Genetics. Dec, 2011  |  Pubmed ID: 21937587

Understanding the transcriptional cues that direct differentiation of human embryonic stem cells (hESCs) and human-induced pluripotent stem cells to defined and functional cell types is essential for future clinical applications. In this study, we have compared transcriptional profiles of haematopoietic progenitors derived from hESCs at various developmental stages of a feeder- and serum-free differentiation method and show that the largest transcriptional changes occur during the first 4 days of differentiation. Data mining on the basis of molecular function revealed Rho-GTPase signalling as a key regulator of differentiation. Inhibition of this pathway resulted in a significant reduction in the numbers of emerging haematopoietic progenitors throughout the differentiation window, thereby uncovering a previously unappreciated role for Rho-GTPase signalling during human haematopoietic development. Our analysis indicated that SCL was the 11th most upregulated transcript during the first 4 days of the hESC differentiation process. Overexpression of SCL in hESCs promoted differentiation to meso-endodermal lineages, the emergence of haematopoietic and erythro-megakaryocytic progenitors and accelerated erythroid differentiation. Importantly, intrasplenic transplantation of SCL-overexpressing hESC-derived haematopoietic cells enhanced recovery from induced acute anaemia without significant cell engraftment, suggesting a paracrine-mediated effect.

Influence of Sodium Bicarbonate on Performance and Hydration in Lightweight Rowing

International Journal of Sports Physiology and Performance. Aug, 2011  |  Pubmed ID: 21941012

PURPOSE: The effect of sodium bicarbonate (NaHCO3) ingestion on pre-race hydration status and 2000 m ergometer performance in elite lightweight rowers was examined using a randomized, cross-over, double-blinded design. METHODS: To simulate body mass (BM) management strategies common to lightweight rowing, oarsmen reduced BM by ~4% in the 24 h preceding trials, and in the 2 h before performance undertook nutritional recovery consisting of mean 43.2 kJ·kg-1, 2.2 g CHO·kg-1, 31.8 mg Na+·kg-1, 24.3 mL H2O·kg-1, and NaHCO3 [0.3 g NaHCO3·kg-1 BM] or placebo [PL (0.15 g cornflour·kg-1 BM)] at 70-90 min before racing. RESULTS: At 25 min before performance, NaHCO3 had increased blood pH (7.48 ± 0.02 vs. PL: 7.41 ± 0.03, P=0.005) and [HCO3 -] (29.1 ± 1.8 vs. PL: 23.9 ± 1.6 mmol·L-1, P<0.001), while BM, urine specific gravity and plasma volume changes were similar between trials. Rowing ergometer times were similar between trials (NaHCO3: 397.8 ± 12.6; PL: 398.6 ± 13.8 s, P=0.417), while post-test [HCO3 -] (11.6 ± 2.3 vs. 9.4 ± 1.8 mmol·L-1, P=0.003) and [lactate-] increases (13.4 ± 1.7 vs. 11.9 ± 1.9 mmol·L-1, P=0.001) were greater with NaHCO3. CONCLUSION: NaHCO3 did not further enhance rehydration or performance in lightweight rowers when undertaking recommended post weigh-in nutritional recovery strategies.

Asymmetric Scalp Electromyogram: a Common and Accurate Lateralizing Sign in Motor Seizures

Journal of Clinical Neurophysiology : Official Publication of the American Electroencephalographic Society. Oct, 2011  |  Pubmed ID: 21946364

The early phase of motor seizures in focal epilepsy produces asymmetry of scalp electromyogram (ASEMG), observable as right-left inequality in muscle artifact on EEG. ASEMG is concordant with clinical lateralization when the seizure has clearly unilateral motor features (tonic, clonic, or versive movements), with higher electromyogram contralateral to the hemisphere of seizure onset. The authors explored whether ASEMG was also present in motor seizures without visible lateralizing signs.

The Polymorphism of Ice: Five Unresolved Questions

Physical Chemistry Chemical Physics : PCCP. Nov, 2011  |  Pubmed ID: 21946782

Our recent discovery of three new phases of ice has increased the total number of known distinct polymorphs of ice to fifteen. In this Perspective article, we give a brief account of previous work in the field, and discuss some of the particularly interesting open questions that have emerged from recent studies. These include (i) the effectiveness of acid and base dopants to enable hydrogen-ordering processes in the ices, (ii) the comparison of the calorimetric data of some of the crystalline phases of ice and low-density amorphous ice, (iii) the disagreement between the experimental ice XV structure and computational predictions, (iv) the incompleteness of some of the hydrogen order/disorder pairs and (v) the new frontiers at the high and negative pressure ends of the phase diagram.

Hyperreninemic Hypertension Following Presumed Abdominal Trauma

Nature Reviews. Nephrology. Dec, 2011  |  Pubmed ID: 21947117

An 18-year-old previously normotensive man was referred to a hypertension unit with blood pressure readings of 140-150/100-110 mmHg. Renal ultrasound had shown a right renal subcapsular fluid collection and an abdominal computed tomography scan had revealed a large cystic lesion surrounding the right kidney with a thick wall and irregular peripheral calcification consistent with a long-standing traumatic perinephric hematoma.

Oxygen Defects and Novel Transport Mechanisms in Apatite Ionic Conductors: Combined 17O NMR and Modeling Studies

Angewandte Chemie (International Ed. in English). Sep, 2011  |  Pubmed ID: 21948428

Performance of Genotype Imputation for Rare Variants Identified in Exons and Flanking Regions of Genes

PloS One. 2011  |  Pubmed ID: 21949800

Genotype imputation has the potential to assess human genetic variation at a lower cost than assaying the variants using laboratory techniques. The performance of imputation for rare variants has not been comprehensively studied. We utilized 8865 human samples with high depth resequencing data for the exons and flanking regions of 202 genes and Genome-Wide Association Study (GWAS) data to characterize the performance of genotype imputation for rare variants. We evaluated reference sets ranging from 100 to 3713 subjects for imputing into samples typed for the Affymetrix (500K and 6.0) and Illumina 550K GWAS panels. The proportion of variants that could be well imputed (true r(2)>0.7) with a reference panel of 3713 individuals was: 31% (Illumina 550K) or 25% (Affymetrix 500K) with MAF (Minor Allele Frequency) less than or equal 0.001, 48% or 35% with 0.0010.05. The performance for common SNPs (MAF>0.05) within exons and flanking regions is comparable to imputation of more uniformly distributed SNPs. The performance for rare SNPs (0.01

Empowering Industrial Research with Shared Biomedical Vocabularies

Drug Discovery Today. Nov, 2011  |  Pubmed ID: 21963522

The life science industries (including pharmaceuticals, agrochemicals and consumer goods) are exploring new business models for research and development that focus on external partnerships. In parallel, there is a desire to make better use of data obtained from sources such as human clinical samples to inform and support early research programmes. Success in both areas depends upon the successful integration of heterogeneous data from multiple providers and scientific domains, something that is already a major challenge within the industry. This issue is exacerbated by the absence of agreed standards that unambiguously identify the entities, processes and observations within experimental results. In this article we highlight the risks to future productivity that are associated with incomplete biological and chemical vocabularies and suggest a new model to address this long-standing issue.

Feasibility of Neonatal Dried Blood Spot Retrieval Amid Evolving State Policies (2009-2010): a Children's Oncology Group Study

Paediatric and Perinatal Epidemiology. Nov, 2011  |  Pubmed ID: 21980944

Dried blood spots (DBS) are collected uniformly from US newborns to test for metabolic and other disorders. Because evidence exists for prenatal origins of some diseases, DBS may provide unique prenatal exposure records. Some states retain residual DBS and permit their use in aetiological studies. The primary study aim was to assess the feasibility of obtaining residual DBS from state newborn screening programmes for paediatric and adolescent cancer patients nationwide with parental/subject consent/assent. Families of leukaemia and lymphoma patients aged ≤21 years diagnosed from 1998 to 2007 at randomly selected Children's Oncology Group institutions across the US were questioned (n = 947). Parents/guardians and patients aged ≥18 years were asked to release DBS to investigators in spring 2009. DBS were then requested from states. Overall, 299 families (32%) released DBS. Consenting/assenting patients were born in 39 US states and 46 DBS were obtained from five states; 124 DBS were unobtainable because patients were born prior to dates of state retention. State policies are rapidly evolving and there is ongoing discussion regarding DBS storage and secondary research uses. Currently, population-based DBS studies can be conducted in a limited number of states; fortunately, many have large populations to provide reasonably sized paediatric subject groups.

Maternal Prenatal Cigarette, Alcohol and Illicit Drug Use and Risk of Infant Leukaemia: a Report from the Children's Oncology Group

Paediatric and Perinatal Epidemiology. Nov, 2011  |  Pubmed ID: 21980945

Several case-control studies have evaluated associations between maternal smoking, alcohol consumption and illicit drug use during pregnancy and risk of childhood leukaemia. Few studies have specifically focused on infants (<1 year) with leukaemia, a group that is biologically and clinically distinct from older children. We present data from a Children's Oncology Group case-control study of 443 infants diagnosed with acute leukaemia [including acute lymphoblastic leukaemia (ALL) and acute myeloid leukaemia (AML)] between 1996 and 2006 and 324 population controls. Mothers were queried about their cigarette, alcohol and illicit drug use 1 year before and throughout pregnancy. Odds ratios (ORs) and 95% confidence intervals [CI] were calculated using adjusted unconditional logistic regression models. Maternal smoking (>1 cigarette/day) and illicit drug use (any amount) before and/or during pregnancy were not significantly associated with infant leukaemia. Alcohol use (>1 drink/week) during pregnancy was inversely associated with infant leukaemia overall [OR = 0.64; 95% CI 0.43, 0.94], AML [OR = 0.49; 95% CI 0.28, 0.87], and leukaemia with mixed lineage leukaemia gene rearrangements ('MLL+') [OR = 0.59; 95% CI 0.36, 0.97]. While our results agree with the fairly consistent evidence that maternal cigarette smoking is not associated with childhood leukaemia, the data regarding alcohol and illicit drug use are not consistent with prior reports and are difficult to interpret. It is possible that unhealthy maternal behaviours during pregnancy, some of which carry potential legal consequences, may not be adequately measured using only self-report. Future case-control studies of childhood leukaemia that pursue these exposures may benefit from incorporation of validated instruments and/or biomarkers when feasible.

Conformer Interconversion in a Switchable Porous Organic Cage

Physical Chemistry Chemical Physics : PCCP. Dec, 2011  |  Pubmed ID: 22002581

Porous organic cage crystals can show unique properties, such as switching from being porous to non-porous in the solid state. Conformer interconversion plays a crucial role in this for the imine cage molecule, CC1. The barriers to interconversion were calculated and found to match experimentally determined values. Calculations suggest that solvent interactions reduce these activation barriers.

Proton Ordering in Cubic Ice and Hexagonal Ice; a Potential New Ice Phase--XIc

Physical Chemistry Chemical Physics : PCCP. Nov, 2011  |  Pubmed ID: 22009223

Ordinary water ice forms under ambient conditions and has two polytypes, hexagonal ice (Ih) and cubic ice (Ic). From a careful comparison of proton ordering arrangements in Ih and Ic using periodic density functional theory (DFT) and diffusion Monte Carlo (DMC) approaches, we find that the most stable arrangement of water molecules in cubic ice is isoenergetic with that of the proton ordered form of hexagonal ice (known as ice XI). We denote this potential new polytype of ice XI as XIc and discuss a possible route for preparing ice XIc.

The Vermiform Appendix Presenting in a Laparoscopic Port Site Hernia

Journal of Minimal Access Surgery. Jul, 2011  |  Pubmed ID: 22022101

Laparoscopic port site hernias (PSHs) are uncommon but present a potential source of morbidity due to incarceration of the hernial contents which is usually omental fat or small bowel. We report only the third case of the vermiform appendix presenting in a symptomatic PSH; we discuss the appropriate management of this condition as well as ways in which the incidence of PSHs may be reduced.

A Simple Device for Multiplex ELISA Made from Melt-extruded Plastic Microcapillary Film

Lab on a Chip. Dec, 2011  |  Pubmed ID: 22030675

We present a simple device for multiplex quantitative enzyme-linked immunosorbant assays (ELISA) made from a novel melt-extruded microcapillary film (MCF) containing a parallel array of 200 μm capillaries along its length. To make ELISA devices different protein antigens or antibodies were immobilised inside individual microcapillaries within long reels of MCF extruded from fluorinated ethylene propylene (FEP). Short pieces of coated film were cut and interfaced with a pipette, allowing sequential uptake of samples and detection solutions into all capillaries from a reagent well. As well as being simple to produce, these FEP MCF devices have excellent light transmittance allowing direct optical interrogation of the capillaries for simple signal quantification. Proof of concept experiments demonstrate both quantitative and multiplex assays in FEP MCF devices using a standard direct ELISA procedure and read using a flatbed scanner. This new multiplex immunoassay platform should find applications ranging from lab detection to point-of-care and field diagnostics.

Comparison of Proton and Electron Radiation Effects on Biological Responses in Liver, Spleen and Blood

International Journal of Radiation Biology. Dec, 2011  |  Pubmed ID: 22035456

To determine whether differences exist between proton and electron radiations on biological responses after total-body exposure.

Minimizing Skin Color Differences Does Not Eliminate the Own-Race Recognition Advantage in Infants

Infancy : the Official Journal of the International Society on Infant Studies. 2011  |  Pubmed ID: 22039335

An abundance of experience with own-race faces and limited to no experience with other-race faces has been associated with better recognition memory for own-race faces in infants, children, and adults. This study investigated the developmental origins of this other-race effect (ORE) by examining the role of a salient perceptual property of faces-that of skin color. Six- and 9-month-olds' recognition memory for own- and other-race faces was examined using infant-controlled habituation and visual-paired comparison at test. Infants were shown own- or other-race faces in color or with skin color cues minimized in grayscale images. Results for the color stimuli replicated previous findings that infants show an ORE in face recognition memory. Results for the grayscale stimuli showed that even when a salient perceptual cue to race, such as skin color information, is minimized, 6- to 9-month-olds, nonetheless, show an ORE in their face recognition memory. Infants' use of shape-based and configural cues for face recognition is discussed.

Development of Face Processing

Wiley Interdisciplinary Reviews. Cognitive Science. 2011  |  Pubmed ID: 22039564

Pathogenic Aberrations Revealed Exclusively by Single Nucleotide Polymorphism (SNP) Genotyping Data in 5000 Samples Tested by Molecular Karyotyping

Journal of Medical Genetics. Dec, 2011  |  Pubmed ID: 22039585

Several recent studies have demonstrated the use of single nucleotide polymorphism (SNP) arrays for the investigation of intellectual disability, developmental delay, autism or congenital abnormalities. In addition to LogR 'copy number' data, these arrays provide SNP genotyping data for gene level autozygosity mapping, estimating low levels of mosaicism, assessing long continuous stretches of homozygosity (LCSH), detection of uniparental disomy, and 'autozygous' regions. However, there remains little specific information on the clinical utility of this genotyping data.

Susceptibility of Standard Clones and European Field Populations of the Green Peach Aphid, Myzus Persicae, and the Cotton Aphid, Aphis Gossypii (Hemiptera: Aphididae), to the Novel Anthranilic Diamide Insecticide Cyantraniliprole

Pest Management Science. Nov, 2011  |  Pubmed ID: 22045565

BACKGROUND: Parthenogenetic clones of the green peach aphid, Myzus persicae (Sulzer), and the cotton aphid, Aphis gossypii Glover, were tested with the anthranilic diamide insecticide cyantraniliprole (i.e. DuPont(™) Cyazypyr(™) ) in systemic-uptake bioassays to investigate potential for cross-resistance conferred by mechanisms of insecticide resistance to organophosphates, carbamates and pyrethroids and, in the case of M. persicae, reduced sensitivity to neonicotinoids. These data were compared with the response of field samples of M. persicae and A. gossypii collected from around Europe. RESULTS: Cyantraniliprole was not cross-resisted by any of the known insecticide resistance mechanisms present in M. persicae or A. gossypii. The compound was equally active against resistant and susceptible aphid strains. The responses of the M. persicae field samples were very consistent with a maximum response ratio of 2.9 compared with a standard laboratory clone. The responses of the A. gossypii field samples were more variable, although a majority of the responses were not statistically different. CONCLUSION: Cyantraniliprole is currently the only anthranilic diamide (IRAC MoA 28) insecticide targeting aphid species such as M. persicae and A. gossypii. There is no evidence to suggest that the performance of this compound is affected by commonly occurring mechanisms that confer resistance to other insecticide chemistries. Cyantraniliprole is therefore a valuable tool for managing insecticide resistance in these globally important pests. Copyright © 2011 Society of Chemical Industry.

Identifying the Presence of Neonicotinoidresistant Peach-potato Aphid (Myzus Persicae) in the Peach-growing Regions of Southern France and Northern Spain

Pest Management Science. Nov, 2011  |  Pubmed ID: 22052736

BACKGROUND: The neonicotinoid class of insecticides is a key component of pest management strategies used by stone fruit producers in Europe. Neonicotinoids are currently one of the most important tools for control of the peach-potato aphid (Myzus persicae). Overreliance on neonicotinoids has led to the development of resistance through a combination of metabolic and target-site resistance mechanisms in individual aphids. A resistance monitoring project was conducted by Syngenta in 2010 to determine the resistance status of M. persicae populations collected from France and Spain, and to determine the frequency of the target-site mutation in those populations. RESULTS: Resistance monitoring suggests that resistance to neonicotinoids is relatively widespread in populations of M. persicae collected from peach orchards in the Languedoc-Roussillon, Provence-Alpes-Cote d'Azur and Rhone-Alpes regions of France, and resistance can be associated with the frequency of the target-site mutation (R81T). The R81T mutation in its heterozygous form is also present in Spanish populations and is associated with neonicotinoid resistance. CONCLUSION: The widespread nature of neonicotinoid resistance in southern France and the potential for resistance development in northern Spain highlight the need for a coordinated management strategy employing insecticides with different modes of action to reduce the selection pressure with neonicotinoids. Copyright © 2011 Society of Chemical Industry.

Sea Cucumber Aquaculture in the Western Indian Ocean: Challenges for Sustainable Livelihood and Stock Improvement

Ambio. Oct, 2011  |  Pubmed ID: 22083524

The decline in sea cucumber fisheries that serve the Asian dried seafood market has prompted an increase in global sea cucumber aquaculture. The tropical sandfish (Holothuria scabra) has, in this context, been reared and produced with mixed success. In the Western Indian Ocean, villagers often participate in the export fishery for sea cucumbers as a source of income. However, with a growing concern of depleted stocks introduction of hatcheries to farm sandfish as a community livelihood and to replenish wild stocks is being promoted. This review identifies and discusses a number of aspects that constitute constraints or implications with regard to development of sandfish farming in the region. The conclusion is that for sandfish farming to live up to its expectations the possible impacts need to be further studied, and that improved evaluation of ongoing projects is required. In the interim, a precautionary approach toward new enterprise activities is suggested.

Successful Implementation of a Perioperative Glycemic Control Protocol in Cardiac Surgery: Barrier Analysis and Intervention Using Lean Six Sigma

Anesthesiology Research and Practice. 2011  |  Pubmed ID: 22091218

Although the evidence strongly supports perioperative glycemic control among cardiac surgical patients, there is scant literature to describe the practical application of such a protocol in the complex ICU environment. This paper describes the use of the Lean Six Sigma methodology to implement a perioperative insulin protocol in a cardiac surgical intensive care unit (CSICU) in a large academic hospital. A preintervention chart audit revealed that fewer than 10% of patients were admitted to the CSICU with glucose <200 mg/dL, prompting the initiation of the quality improvement project. Following protocol implementation, more than 90% of patients were admitted with a glucose <200 mg/dL. Key elements to success include barrier analysis and intervention, provider education, and broadening the project scope to address the intraoperative period.

Impact of Operating History on Mixed Culture Fermentation Microbial Ecology and Product Mixture

Water Science and Technology : a Journal of the International Association on Water Pollution Research. 2011  |  Pubmed ID: 22097058

Mixed culture fermentation is an alternative to pure culture fermentation for production of biofuels and valuable products. A glucose-fed, continuous reactor was operated cyclically to a central pH of 5.5 from a number of precedent pHs, from 4.5 to 7.5. At each pH, stable chemical production was reached after 2 retention times and was held for least 2 further retention times prior to the next change. Bacterial groups were identified by phylogenetic analysis of 16S rRNA gene clones. Bacterial community dynamics were monitored by terminal-restriction fragment length polymorphism. More ethanol was produced at high pH, and more butyrate at lower pH. At pH 5.5, the product spectrum was not measurably influenced by precedent pH but showed seemingly random changes. The impact of precedent pH on community structure was more systematic, with clear indications that when the pH was returned to 5.5, the bacterial group that was dominant at the precedent pH remained at high abundance. This result is important, since it indicates a decoupling between microbial function (as indicated by product spectrum), and community structure. More work is needed to determine the longevity of this hysteresis effect. There was evidence that groups retained their ability to re-emerge even after times of low abundance.

Maxillary Sinus Lift with Solely Autogenous Bone Compared to a Combination of Autogenous Bone and Growth Factors or (solely) Bone Substitutes. A Systematic Review

International Journal of Oral and Maxillofacial Surgery. Nov, 2011  |  Pubmed ID: 22099314

Literature regarding the outcome of maxillary sinus floor elevation to create sufficient bone fraction to enable implant placement was systematically reviewed. Bone fraction and implant survival rate were assessed to determine whether grafting material or applied growth factor affected bone fraction. Trials where sinus floor elevations with autogenous bone (controls) were compared with autogenous bone combined with growth factors or bone substitutes, or solely with bone substitutes (test groups) were identified; 12 of 1124 fulfilled all inclusion criteria. Meta-analyses comparing the bone fraction after applying: autogenous bone; autologous bone with growth factors (platelet rich plasma); or autogenous bone and bone substitutes (bovine hydroxyapatite, bioactive glass, corticocancellous pig bone) revealed no significant differences in bone formation after 5 months. A significantly higher bone fraction was found in the autogenous bone group compared to the sole use of β-tricalciumphosphate (P=0.036). The one-year overall implant survival rate showed no significant difference between implants. Bone substitutes combined with autogenous bone provide a reliable alternative for autogenous bone as sole grafting material to reconstruct maxillary sinus bony deficiencies, for supporting dental implants after 5 months. Adding growth factors (platelet rich plasma) to grafting material and the sole use of β-tricalciumphosphate did not promote bone formation.

Hydrogen Bonds and Van Der Waals Forces in Ice at Ambient and High Pressures

Physical Review Letters. Oct, 2011  |  Pubmed ID: 22107644

The first principles methods, density-functional theory and quantum Monte Carlo, have been used to examine the balance between van der Waals (vdW) forces and hydrogen bonding in ambient and high-pressure phases of ice. At higher pressure, the contribution to the lattice energy from vdW increases and that from hydrogen bonding decreases, leading vdW to have a substantial effect on the transition pressures between the crystalline ice phases. An important consequence, likely to be of relevance to molecular crystals in general, is that transition pressures obtained from density-functional theory exchange-correlation functionals which neglect vdW forces are greatly overestimated.

Geographical Variation in Egg Mass and Egg Content in a Passerine Bird

PloS One. 2011  |  Pubmed ID: 22110579

Reproductive, phenotypic and life-history traits in many animal and plant taxa show geographic variation, indicating spatial variation in selection regimes. Maternal deposition to avian eggs, such as hormones, antibodies and antioxidants, critically affect development of the offspring, with long-lasting effects on the phenotype and fitness. Little is however known about large-scale geographical patterns of variation in maternal deposition to eggs. We studied geographical variation in egg components of a passerine bird, the pied flycatcher (Ficedula hypoleuca), by collecting samples from 16 populations and measuring egg and yolk mass, albumen lysozyme activity, yolk immunoglobulins, yolk androgens and yolk total carotenoids. We found significant variation among populations in most egg components, but ca. 90% of the variation was among individuals within populations. Population however explained 40% of the variation in carotenoid levels. In contrast to our hypothesis, we found geographical trends only in carotenoids, but not in any of the other egg components. Our results thus suggest high within-population variation and leave little scope for local adaptation and genetic differentiation in deposition of different egg components. The role of these maternally-derived resources in evolutionary change should be further investigated.

The Entomopathogenic Bacterial Endosymbionts Xenorhabdus and Photorhabdus: Convergent Lifestyles from Divergent Genomes

PloS One. 2011  |  Pubmed ID: 22125637

Members of the genus Xenorhabdus are entomopathogenic bacteria that associate with nematodes. The nematode-bacteria pair infects and kills insects, with both partners contributing to insect pathogenesis and the bacteria providing nutrition to the nematode from available insect-derived nutrients. The nematode provides the bacteria with protection from predators, access to nutrients, and a mechanism of dispersal. Members of the bacterial genus Photorhabdus also associate with nematodes to kill insects, and both genera of bacteria provide similar services to their different nematode hosts through unique physiological and metabolic mechanisms. We posited that these differences would be reflected in their respective genomes. To test this, we sequenced to completion the genomes of Xenorhabdus nematophila ATCC 19061 and Xenorhabdus bovienii SS-2004. As expected, both Xenorhabdus genomes encode many anti-insecticidal compounds, commensurate with their entomopathogenic lifestyle. Despite the similarities in lifestyle between Xenorhabdus and Photorhabdus bacteria, a comparative analysis of the Xenorhabdus, Photorhabdus luminescens, and P. asymbiotica genomes suggests genomic divergence. These findings indicate that evolutionary changes shaped by symbiotic interactions can follow different routes to achieve similar end points.

Hospital Administration Team Development and Support in a Children's Cancer Service

Australian Health Review : a Publication of the Australian Hospital Association. Nov, 2011  |  Pubmed ID: 22126946

The administration team in the Queensland Children's Cancer Centre at the Royal Children's Hospital, Brisbane, included a team of 16 administration staff supporting the service and the state-wide clinical network. Shortly after the creation of a new expanded service, issues became apparent in administration team morale, relationships, communication, processes, leadership, support and training. The analysis of these issues included team interviews and surveys, consultation with senior administration staff and monthly sick leave monitoring. Strategies implemented included providing information; the joint development of a team business plan and individual performance plans; a review of the team's structure, workload and business processes; engaging staff in quality improvements; and the development of relationships and leadership. As a result, the team reported being more comfortable and supported in their roles, had improved morale and worked better together with more consistent and improving business processes. They had clear purpose and expectations of their roles, displayed better customer service and had reduced sick leave. The study shows that in a high stress environment, such as a children's cancer centre, attention to the team's culture, vision and purpose, providing information and improving communication and relationships, when combined with a team's enthusiasm, will improve the team's growth, cooperation and work outcomes.

Experimental Loss-tolerant Quantum Coin Flipping

Nature Communications. 2011  |  Pubmed ID: 22127057

Coin flipping is a cryptographic primitive in which two distrustful parties wish to generate a random bit to choose between two alternatives. This task is impossible to realize when it relies solely on the asynchronous exchange of classical bits: one dishonest player has complete control over the final outcome. It is only when coin flipping is supplemented with quantum communication that this problem can be alleviated, although partial bias remains. Unfortunately, practical systems are subject to loss of quantum data, which allows a cheater to force a bias that is complete or arbitrarily close to complete in all previous protocols and implementations. Here we report on the first experimental demonstration of a quantum coin-flipping protocol for which loss cannot be exploited to cheat better. By eliminating the problem of loss, which is unavoidable in any realistic setting, quantum coin flipping takes a significant step towards real-world applications of quantum communication.

A Randomized Controlled Trial of Cognitive-behavior Therapy Plus Bright Light Therapy for Adolescent Delayed Sleep Phase Disorder

Sleep. 2011  |  Pubmed ID: 22131604

To evaluate cognitive-behavior therapy plus bright light therapy (CBT plus BLT) for adolescents diagnosed with delayed sleep phase disorder (DSPD).

Exposure Circumstances and Outcomes of 48 Households with 57 Cats Exposed to Toxic Lily Species

Journal of the American Animal Hospital Association. Nov, 2011  |  Pubmed ID: 22058344

Ingestion of Lilium or Hemerocallis spp. by cats can result in renal failure. The objectives of this study were to determine the foreknowledge of lily toxicity of owners of cats that were exposed to lilies and to obtain historical, clinical and outcome information on the exposures. A survey was done of cat owners reporting indoor exposures to lilies to the Animal Poison Control Center (APCC) during April 2009. Forty eight individuals, (57 cats) were included. Sixty nine percent of cat owners said they could recognize a lily and 27% knew that lilies were toxic prior to their cats' exposures. Most lilies were obtained from grocery or other stores, and were purchased by the owners or as gifts to the cat owners. Owners who were unaware of lily toxicity frequently left the flowers where the cats had access to them, whereas in households where the toxicity was known the cats actively sought out the flowers. Of the cats in this study 93% received prompt veterinary care, and 87% either developed no signs or had brief signs that resolved. Five percent had evidence of renal insufficiency at final follow-up and another 5 percent of cats were euthanized due to renal failure.

Spontaneous Regression of Large Mandibular Lesions: Consider Langerhans Cell Histiocytosis

Dento Maxillo Facial Radiology. Dec, 2011  |  Pubmed ID: 22065805

A Perylene Diimide Rotaxane: Synthesis, Structure and Electrochemically Driven De-threading

Chemistry (Weinheim an Der Bergstrasse, Germany). Dec, 2011  |  Pubmed ID: 22135247

The first example of a [2]-rotaxane in which a perylene diimide acts as a recognition site has been synthesised and characterised. The interlocked nature of the compound has been verified by both NMR studies and an X-ray structure determination. Electrochemical investigations confirm that the nature of the redox processes associated with the perylene diimide are modified by the complexation process and that it is possible to mono-reduce the [2]-rotaxane to give a radical anion based rotaxane. Further reduction of the compound leads to de-threading of the macrocycle from the reduced PTCDI recognition site. Our synthetic strategies confirm the potential of PTCDI-based rotaxanes as viable targets for the preparation of complex interlocked species.

The Impact of State Laws and District Policies on Physical Education and Recess Practices in a Nationally Representative Sample of US Public Elementary Schools

Archives of Pediatrics & Adolescent Medicine. Dec, 2011  |  Pubmed ID: 22147763

OBJECTIVE: To examine the impact of state- and school district-level policies on the prevalence of physical education (PE) and recess in a nationally representative sample of US public elementary schools. DESIGN: Analyses from annual, nationally representative, cross-sectional surveys of school administrators in the United States. SETTING: Data were collected through surveys conducted between February and June during the 2006-2007 through 2008-2009 school years. State laws and district policies were compiled annually by researchers at the University of Illinois at Chicago using established legal research techniques. PARTICIPANTS: The sample size was 47 states, 690 districts, and 1761 schools. Main Exposures  State- and school district-level PE and recess-related laws MAIN OUTCOME MEASURES: Twenty minutes of daily recess and 150 min/wk of PE. RESULTS: The odds of schools having 150 min/wk of PE increased if they were located in states (odds ratio [OR], 2.8; 95% CI, 1.3-5.7) or school districts (OR, 2.4; 95% CI, 1.3-4.3) having a law or policy requiring 150 min/wk of PE. Schools located in states with laws encouraging daily recess were significantly more likely to have 20 minutes of recess daily (OR, 1.8; 95% CI, 1.2-2.8). District policies were not significantly associated with school-level recess practices. Adequate PE time was inversely associated with recess and vice versa, suggesting that schools are substituting one form of physical activity for another rather than providing the recommended amount of both recess and PE. CONCLUSION: By mandating PE or recess, policy makers can effectively increase school-based physical activity opportunities for youth.

Phenotypic Variation and Host Interactions of Xenorhabdus Bovienii SS-2004, the Entomopathogenic Symbiont of Steinernema Jollieti Nematodes

Environmental Microbiology. Dec, 2011  |  Pubmed ID: 22151385

Xenorhabdus bovienii (SS-2004) bacteria reside in the intestine of the infective-juvenile (IJ) stage of the entomopathogenic nematode, Steinernema jollieti. The recent sequencing of the X. bovienii genome facilitates its use as a model to understand host - symbiont interactions. To provide a biological foundation for such studies, we characterized X. bovienii in vitro and host interaction phenotypes. Within the nematode host X. bovienii was contained within a membrane bound envelope that also enclosed the nematode-derived intravesicular structure. Steinernema jollieti nematodes cultivated on mixed lawns of X. bovienii expressing green or DsRed fluorescent proteins were predominantly colonized by one or the other strain, suggesting the colonizing population is founded by a few cells. Xenorhabdus bovienii exhibits phenotypic variation between orange-pigmented primary form and cream-pigmented secondary form. Each form can colonize IJ nematodes when cultured in vitro on agar. However, IJs did not develop or emerge from Galleria mellonella insects infected with secondary form. Unlike primary-form infected insects that were soft and flexible, secondary-form infected insects retained a rigid exoskeleton structure. Xenorhabdus bovienii primary and secondary form isolates are virulent towards Manduca sexta and several other insects. However, primary form stocks present attenuated virulence, suggesting that X. bovienii, like Xenorhabdus nematophila may undergo virulence modulation.

Laxative-free CT Colonography

The British Journal of Radiology. Dec, 2011  |  Pubmed ID: 22167512

Objectives: To determine if the introduction of faecal tagging to CT colonography (CTC) made the examination easier to tolerate, or reduced the number of false positives.Methods: Our department changed bowel preparation for CT colonography from Picolax to Gastrografin only with a modified diet. Questionnaires were given to a subgroup of patients within these cohorts. The numbers of false positives were compared between two cohorts before and after this change. False positives were defined as lesions reported on CT that were not confirmed by subsequent endoscopic examination. Polyps were matched if they were in the same or adjacent segments, and were within 5 mm of the reported size.Results: 412 patients were identified from the Picolax cohort, and 116 from the Gastrografin cohort. 62 patients in each group completed questionnaires. Gastrografin produced less diarrhoea; 34% had five or more bowel motions in the previous day and night, compared with 77% for Picolax (p<0.001), although more patients found drinking it unpleasant compared with Picolax (85% reported drinking Picolax as "easy" vs 61% for Gastrografin; P0.002). Picolax produced more non-diagnostic examinations, although this difference was not statistically significant. There was not a significant reduction in the numbers of false positives (2 out of 112 for Gastrografin group, 14 out of 389 for the Picolax group; p = 0.54).Conclusion: Switching from Picolax to Gastrografin as a CTC preparation technique produced less diarrhoea, but did not reduce the number of false positives.

Electronic Structure of Lithium Battery Interphase Compounds: Comparison Between Inelastic X-ray Scattering Measurements and Theory

The Journal of Chemical Physics. Dec, 2011  |  Pubmed ID: 22168709

In lithium ion batteries, decomposition of the electrolyte and its associated passivation of the electrode surface occurs at low potentials, resulting in an electronically insulating, but Li-ion conducting, solid electrolyte interphase (SEI). The products of the SEI and their chemical constituents/properties play an important role in the long-term stability and performance of the battery. Reactivity and the sub-keV core binding energies of lithium, carbon, oxygen, and fluorine species in the SEI present technical challenges in the spectroscopy of these compounds. Using an alternative approach, nonresonant inelastic x-ray scattering, we examine the near-edge spectra of bulk specimens of common SEI compounds, including LiF, Li(2)CO(3), LiOH, LiOH·H(2)O, and Li(2)O. By working at hard x-ray energies, we also experimentally differentiate the s- and p-symmetry components of lithium's unoccupied states using the evolution of its K edge with momentum transfer. We find good agreement with theoretical spectra calculated using a Bethe-Salpeter approach in all cases. These results provide an analytical and diagnostic foundation for better understanding of the makeup of SEIs and the mechanism of their formation.

Search for the Rare Decays K_{L}→π^{0}π^{0}μ^{+}μ^{-} and K_{L}→π^{0}π^{0}X^{0}→π^{0}π^{0}μ^{+}μ^{-}

Physical Review Letters. Nov, 2011  |  Pubmed ID: 22181724

The KTeV E799 experiment has conducted a search for the rare decays, K_{L}→π^{0}π^{0}μ^{+}μ^{-} and K_{L}→π^{0}π^{0}X^{0}→π^{0}π^{0}μ^{+}μ^{-}, where the X^{0} is a possible new neutral boson that was reported by the HyperCP experiment with a mass of (214.3±0.5)  MeV/c^{2}. We find no evidence for either decay. We obtain upper limits of Br(K_{L}→π^{0}π^{0}X^{0}→π^{0}π^{0}μ^{+}μ^{-})<1.0×10^{-10} and Br(K_{L}→π^{0}π^{0}μ^{+}μ^{-})<9.2×10^{-11} at the 90% confidence level. This result rules out the pseudoscalar X^{0} as an explanation of the HyperCP result under the scenario that the d[over ¯]sX^{0} coupling is completely real.

Search for a Standard Model Higgs Boson in the H→ZZ→ℓ(+)ℓ(-)νν Decay Channel with the ATLAS Detector

Physical Review Letters. Nov, 2011  |  Pubmed ID: 22182021

A search for a heavy standard model Higgs boson decaying via H→ZZ→→ℓ(+)ℓ(-)νν, where ℓ=e, μ, is presented. It is based on proton-proton collision data at √s=7 TeV, collected by the ATLAS experiment at the LHC in the first half of 2011 and corresponding to an integrated luminosity of 1.04 fb(-1). The data are compared to the expected standard model backgrounds. The data and the background expectations are found to be in agreement and upper limits are placed on the Higgs boson production cross section over the entire mass window considered; in particular, the production of a standard model Higgs boson is excluded in the region 340

Search for the Higgs Boson in the H→WW→lνjj Decay Channel in Pp Collisions at ˆ�s=7 TeV with the ATLAS Detector

Physical Review Letters. Dec, 2011  |  Pubmed ID: 22182080

A search for a Higgs boson has been performed in the H→WW→ℓνjj channel in 1.04 fb(-1) of pp collision data at √s=7 TeV recorded with the ATLAS detector at the Large Hadron Collider. No significant excess of events is observed over the expected background and limits on the Higgs boson production cross section are derived for a Higgs boson mass in the range 240 GeV

Validation of Secondary Commercial Data Sources for Physical Activity Facilities in Urban and Non-Urban Settings

Journal of Physical Activity & Health. Dec, 2011  |  Pubmed ID: 22207600

BACKGROUND: Secondary data is often necessary to assess the availability of commercial physical activity (PA) facilities and examine its association with individual behaviors and outcomes, yet the validity of such sources has been explored only in a limited number of studies. METHODS: Field data were collected on the presence and attributes of commercial PA facilities in a random sample of 30 urban, 15 suburban and 15 rural Census tracts in the Chicago metropolitan statistical area and surrounding area. RESULTS: Approximately 40% of PA establishments in the field data were listed for both urban and non-urban tracts in both lists except for non-urban tracts in D&B (35%), which was significantly improved in the combined list of D&B and InfoUSA. Approximately one quarter of the PA facilities listed in D&B were found on the ground in D&B whereas 40-50% of PA facilities listed in InfoUSA were found on the ground. PA establishments that offered instruction programs or lessons or that had a court or pool were less likely to be listed, particularly in the non-urban tracts. CONCLUSIONS: Secondary commercial business lists on PA facilities should be used with caution in assessing the built environment.

An Akt-dependent Increase in Canonical Wnt Signaling and a Decrease in Sclerostin Protein Levels Are Involved in Strontium Ranelate-induced Osteogenic Effects in Human Osteoblasts

The Journal of Biological Chemistry. Jul, 2011  |  Pubmed ID: 21566129

Sclerostin is an important regulator of bone homeostasis and canonical Wnt signaling is a key regulator of osteogenesis. Strontium ranelate is a treatment for osteoporosis that has been shown to reduce fracture risk, in part, by increasing bone formation. Here we show that exposure of human osteoblasts in primary culture to strontium increased mineralization and decreased the expression of sclerostin, an osteocyte-specific secreted protein that acts as a negative regulator of bone formation by inhibiting canonical Wnt signaling. Strontium also activated, in an apparently separate process, an Akt-dependent signaling cascade via the calcium-sensing receptor that promoted the nuclear translocation of β-catenin. We propose that two discrete pathways linked to canonical Wnt signaling contribute to strontium-induced osteogenic effects in osteoblasts.

Factors Affecting Valrubicin Response in Patients with Bacillus Calmette-Guérin-refractory Bladder Carcinoma in Situ

Postgraduate Medicine. May, 2011  |  Pubmed ID: 21566413

Patients with bacillus Calmette-Guérin (BCG)-refractory carcinoma in situ (CIS) of the bladder are candidates for intravesical (IVe) valrubicin. This post-hoc analysis of data from the pivotal phase 3, prospective, open-label study of valrubicin evaluated the effects of patient characteristics and past treatments on the response to valrubicin.

Melting the Ice: on the Relation Between Melting Temperature and Size for Nanoscale Ice Crystals

ACS Nano. Jun, 2011  |  Pubmed ID: 21568289

Although the melting of ice is an everyday process, important issues remain unclear particularly on the nanoscale. Indeed despite extensive studies into ice melting and premelting, little is known about the relationship between (pre)melting and crystal size and morphology, with, for example, the melting temperature of ice nanocrystals being unclear. Here we report extensive long-time force-field-based molecular dynamics studies of the melting of hexagonal ice nanocrystals in the ca. 2 to 8 nm size range. We show that premelting is initiated at the corners of the crystals, then the edges between facets, and then the flat surfaces; that is, the melting temperature is related to the degree of coordination. A strong size dependence of the melting temperature is observed, with the combination of small particle size and premelting leading nanosized ice crystals to have liquid-like surfaces as low as about 130 K below the bulk ice melting temperature. These results will be of relevance in understanding the size dependence of ice crystal morphology and the surface reactivity of ice particles under atmospheric conditions.

Cognitive Correlates of Psychosocial Outcome Following Traumatic Brain Injury in Early Childhood: Comparisons Between Groups of Children Aged Under and over 10 Years of Age

Clinical Child Psychology and Psychiatry. Apr, 2011  |  Pubmed ID: 21571762

Children with traumatic brain injuries (TBI) commonly present with socioemotional difficulties, as well as accompanying multiple cognitive impairments. Often difficulties worsen at around 10 years old. This change is associated with frontal system changes, and tests of executive function (EF) predict outcome. However, children with TBI sometimes present with socioemotional difficulties despite apparent cognitive recovery. Our aims were to explore potential cognitive and socioemotional effects following childhood TBI, before and after the age of 10 years. We also wanted to identify cognitive correlates of psychosocial dysfunction. Measures of cognitive function and socioemotional disturbance administered to 14 children with TBI aged 8-10 years, and 14 children with TBI aged 10-16 years, were compared to control data from 22 non-injured 8- to 10 year-olds and 67 non-injured 10- to 16-year-olds. Results indicated that only the older group of children with TBI were impaired in tests of EF, but significant socioemotional difficulties were commonly evident in both groups. Processing speed (as well as EF) was found to correlate with socioemotional disturbance. We conclude that poor processing speed may also index the risk of socioemotional difficulties, but our general findings indicate that cognitive functions relevant to socioemotional functioning are not readily testable in younger children and are not strongly associated with such outcomes as they may be in adults.

Looking Across Domains to Understand Infant Representation of Emotion

Emotion Review. Apr, 2011  |  Pubmed ID: 21572929

A comparison of the literatures on how infants represent generic object classes, gender and race information in faces, and emotional expressions reveals both common and distinctive developments in the three domains. In addition, the review indicates that some very basic questions remain to be answered regarding how infants represent facial displays of emotion, including (a) whether infants form category representations for discrete classes of emotion, when and how such representations come(b) to incorporate affective meaning, (c) the developmental trajectory for representation of emotional expression at different levels of inclusiveness (i.e., from broad to narrow or narrow to broad?), and (d) whether there is superior discrimination ability operating within more frequently experienced emotion categories.

[Calibration Study of the Food Frequency Questionnaire for Adolescents (AFFQ)]

Ciência & Saúde Coletiva. Apr, 2011  |  Pubmed ID: 21584475

In order to establish calibration factors of the Adolescent Food Frequency Questionnaire (AFFQ), 74 boys and girls from Piracicaba (SP, Brazil) with ages ranging from 10 to 14 took part in the study. Dietary intake (assessed by the food frequency questionnaire and 24-hour recall) was assessed and adjusted for energy intake. Descriptive statistics, variance analysis using one classification factor, Pearson's correlation coefficients and linear regression were performed. The average of two 24-hour recalls was used as a reference for calibration of data. Calibration coefficients (λ) ranged from -0.07 (iron) to 0.40 (vitamin C) revealing substantial error in the dietary method tested, albeit similar to those observed in the literature. As these coefficients were low, they indicate the need for reformulating the instrument regarding some nutrients, though application was not considered advisable for correcting information on iron and retinol. The methodology used to calibrate dietary data can consider measurement error in the assessment when its assumptions are respected, since violations of these assumptions may lead to other errors that are difficult to predict.

A Randomized, Double-blinded, Placebo-controlled, Crossover, Add-on Study of CoEnzyme Q10 in the Prevention of Pediatric and Adolescent Migraine

Cephalalgia : an International Journal of Headache. Jun, 2011  |  Pubmed ID: 21586650

To evaluate the efficacy of Coenzyme Q10 (CoQ10) supplementation in the prevention of migraine in children using a placebo-controlled, double-blinded, crossover, add-on trial.

Childhood Abuse in Pediatric Patients With Chronic Daily Headache

Clinical Pediatrics. May, 2011  |  Pubmed ID: 21593054

Assessing the Potential for Rhizoremediation of PCB Contaminated Soils in Northern Regions Using Native Tree Species

Chemosphere. Jun, 2011  |  Pubmed ID: 21596420

Rhizosphere bioremediation of polychlorinated biphenyls (PCBs) offers a potentially inexpensive approach to remediating contaminated soils that is particularly attractive in remote regions including the Arctic. We assessed the abilities of two tree species native to Alaska, Salix alaxensis (felt-leaf willow) and Picea glauca (white spruce), to promote microbial biodegradation of PCBs via the release of phytochemicals upon fine root death. Crushed fine roots, biphenyl (PCB analogue) or salicylate (willow secondary compound) were added to microcosms containing soil spiked with PCBs and resultant PCB disappearance, soil toxicity and microbial community changes were examined. After 180d, soil treated with willow root crushates showed a significantly greater PCB loss than untreated soils for some PCB congeners, including the toxic congeners, PCB 77, 105 and 169, and showed a similar PCB loss pattern (in both extent of degradation and congeners degraded) to biphenyl-treated microcosms. Neither P. glauca (white spruce) roots nor salicylate enhanced PCB loss, indicating that biostimulation is plant species specific and was not mediated by salicylate. Soil toxicity assessed using the Microtox bioassay indicated that the willow treatment resulted in a less toxic soil environment. Molecular microbial community analyses indicated that biphenyl and salicylate promoted shifts in microbial community structure and composition that differed distinctly from each other and from the crushed root treatments. The biphenyl utilizing bacterium, Cupriavidus spp. was isolated from the soil. The findings suggest that S. alaxensis may be an effective plant for rhizoremediation by altering microbial community structure, enhancing the loss of some PCB congeners and reducing the toxicity of the soil environment.

Perceptual Training Prevents the Emergence of the Other Race Effect During Infancy

PloS One. 2011  |  Pubmed ID: 21625638

Experience plays a crucial role in the development of the face processing system. At 6 months of age infants can discriminate individual faces from their own and other races. By 9 months of age this ability to process other-race faces is typically lost, due to minimal experience with other-race faces, and vast exposure to own-race faces, for which infants come to manifest expertise [1]. This is known as the Other Race Effect. In the current study, we demonstrate that exposing Caucasian infants to Chinese faces through perceptual training via picture books for a total of one hour between 6 and 9 months allows Caucasian infants to maintain the ability to discriminate Chinese faces at 9 months of age. The development of the processing of face race can be modified by training, highlighting the importance of early experience in shaping the face representation.

Mutation of a Nicotinic Acetylcholine Receptor β Subunit is Associated with Resistance to Neonicotinoid Insecticides in the Aphid Myzus Persicae

BMC Neuroscience. 2011  |  Pubmed ID: 21627790

Myzus persicae is a globally important aphid pest with a history of developing resistance to insecticides. Unusually, neonicotinoids have remained highly effective as control agents despite nearly two decades of steadily increasing use. In this study, a clone of M. persicae collected from southern France was found, for the first time, to exhibit sufficiently strong resistance to result in loss of the field effectiveness of neonicotinoids.

Resilience and the Mediating Effects of Executive Dysfunction After Childhood Brain Injury: a Comparison Between Children Aged 9-15 Years with Brain Injury and Non-injured Controls

Brain Injury : [BI]. 2011  |  Pubmed ID: 21631188

Acquired brain injury (ABI) during childhood can be associated with enduring difficulties related to impairments to executive functioning (EF). EF impairments may detrimentally affect outcome by restricting an individual's ability to access 'resiliency' resources after ABI.

Measurement of Dijet Azimuthal Decorrelations in Pp Collisions at Sqrt(s)=7  TeV

Physical Review Letters. Apr, 2011  |  Pubmed ID: 21635030

Azimuthal decorrelations between the two central jets with the largest transverse momenta are sensitive to the dynamics of events with multiple jets. We present a measurement of the normalized differential cross section based on the full data set (∫Ldt=36  pb(-1)) acquired by the ATLAS detector during the 2010 sqrt(s)=7  TeV proton-proton run of the LHC. The measured distributions include jets with transverse momenta up to 1.3 TeV, probing perturbative QCD in a high-energy regime.

Laparoscopic Duodenojejunostomy for Superior Mesenteric Artery Syndrome

Digestive Diseases and Sciences. Sep, 2011  |  Pubmed ID: 21643740

Effects of Adverts from a Drug and Alcohol Prevention Campaign on Willingness to Engage in Alcohol-related Risky Behaviors

Journal of Health Psychology. Nov, 2011  |  Pubmed ID: 21646292

Behavioral willingness is conceptualized as a pathway to behavior that is non-deliberative, yet traditional measures require thoughtful deliberation to complete. This study explored non-deliberative measures of alcohol-related willingness to complement recent work on marijuana-related willingness. The study also examined whether adverts from a field-tested drug and alcohol prevention campaign may have operated by influencing alcohol-related willingness. Participants viewed campaign adverts or consumer adverts (control). Outcomes were reaction times to make speeded judgments about whether one would engage in risky alcohol-related behaviors. Results showed that campaign advertisements lowered willingness to play drinking games and (for males) to drive while intoxicated.

Origin and Evolution of Prebiotic Organic Matter As Inferred from the Tagish Lake Meteorite

Science (New York, N.Y.). Jun, 2011  |  Pubmed ID: 21659601

The complex suite of organic materials in carbonaceous chondrite meteorites probably originally formed in the interstellar medium and/or the solar protoplanetary disk, but was subsequently modified in the meteorites' asteroidal parent bodies. The mechanisms of formation and modification are still very poorly understood. We carried out a systematic study of variations in the mineralogy, petrology, and soluble and insoluble organic matter in distinct fragments of the Tagish Lake meteorite. The variations correlate with indicators of parent body aqueous alteration. At least some molecules of prebiotic importance formed during the alteration.

Nutrition Guidelines for Strength Sports: Sprinting, Weightlifting, Throwing Events, and Bodybuilding

Journal of Sports Sciences. 2011  |  Pubmed ID: 21660839

Strength and power athletes are primarily interested in enhancing power relative to body weight and thus almost all undertake some form of resistance training. While athletes may periodically attempt to promote skeletal muscle hypertrophy, key nutritional issues are broader than those pertinent to hypertrophy and include an appreciation of the sports supplement industry, the strategic timing of nutrient intake to maximize fuelling and recovery objectives, plus achievement of pre-competition body mass requirements. Total energy and macronutrient intakes of strength-power athletes are generally high but intakes tend to be unremarkable when expressed relative to body mass. Greater insight into optimization of dietary intake to achieve nutrition-related goals would be achieved from assessment of nutrient distribution over the day, especially intake before, during, and after exercise. This information is not readily available on strength-power athletes and research is warranted. There is a general void of scientific investigation relating specifically to this unique group of athletes. Until this is resolved, sports nutrition recommendations for strength-power athletes should be directed at the individual athlete, focusing on their specific nutrition-related goals, with an emphasis on the nutritional support of training.

Children's Discrimination of Fantastic Vs. Realistic Visual Displays After Watching a Film with Magical Content

Perceptual and Motor Skills. Apr, 2011  |  Pubmed ID: 21667768

Six- and nine-yr.-old children (n=28 of each) were divided into equal experimental and control groups. The experimental groups were shown a film with a magical theme, and the control groups were shown a film with a nonmagical theme. All groups then were presented with a choice task requiring them to discriminate between ordinary and fantastic visual displays on a computer screen. Statistical analyses indicated that mean scores for correctly identifying the ordinary and fantastic displays were significantly different between experimental and control groups. The children in the experimental groups who watched the magical film had significantly higher scores on correct identifications than children in the control groups who watched the nonmagical film for both age groups. The results suggest that watching films with a magical theme might enhance children's sensitivity toward the fantasy/reality distinction.

Left Renal Vein Ligation: a Technique to Mitigate Low Portal Flow from Splenic Vein Siphon During Liver Transplantation

American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons. Aug, 2011  |  Pubmed ID: 21668639

Low portal vein flows in liver transplant have been associated with poor allograft survival. Identifying and ameliorating causes of inadequate portal flow is paramount. We describe successful reversal of significant splenic vein siphon from a spontaneous splenorenal shunt during liver transplant. The patient is a 43-year-old male with cirrhosis from hepatitis C and Budd-Chiari syndrome, who had a variceal hemorrhage necessitating an emergent splenorenal shunt with 8 mm PTFE graft. Imaging in 2006 revealed thrombosis of the splenorenal shunt and evidence of a new spontaneous splenorenal shunt. The patient developed hepatocellular carcinoma and underwent transplant in 2009. After reperfusion, portal flows were low (150-200 mL/min). A mesenteric varix was ligated without improvement. Due to adhesions, direct collateral ligation was not attempted. In order to redirect the splenic siphon, the left renal vein was stapled at its confluence with the inferior vena cava. Portal flows subsequently increased to 1.28 L/min. Postoperatively, the patient had stable renal and liver function. We conclude that spontaneous splenorenal shunts can cause low portal flows. A diligent search for shunts with understanding of flow patterns is critical; ligation or rerouting of splanchnic flow may be necessary to improve portal flows and allograft outcomes.

Phenotypic Variability of Distal 22q11.2 Copy Number Abnormalities

American Journal of Medical Genetics. Part A. Jul, 2011  |  Pubmed ID: 21671380

The availability of microarray technology has led to the recent recognition of copy number abnormalities of distal chromosome 22q11.2 that are distinct from the better-characterized deletions and duplications of the proximal region. This report describes five unrelated individuals with copy number abnormalities affecting distal chromosome 22q11.2. We report on novel phenotypic features including diaphragmatic hernia and uterine didelphys associated with the distal microdeletion syndrome; and frontomedial polymicrogyria and callosal agenesis associated with the distal microduplication syndrome. We describe the third distal chromosome 22q11.2 microdeletion patient with Goldenhar syndrome. Patients with distal chromosome 22q11.2 copy number abnormalities exhibit inter- and intra-familial phenotypic variability, and challenge our ability to draw meaningful genotype-phenotype correlations.

Molecular Epidemiology of Strangles Outbreaks in the UK During 2010

The Veterinary Record. Jun, 2011  |  Pubmed ID: 21672953

The sequence of the Streptococcus equi subspecies equi (S equi) M-like protein (SeM) gene was determined for 105 isolates of S equi from strangles outbreaks in the UK during 2010 and compared with previous data from 2007 to 2008. Twenty-three distinct alleles were identified, including 11 novel alleles. One allele giving rise to a putative truncated M protein was identified from the guttural pouch of an asymptomatic carrier. Allele 9 was the most prevalent, comprising 57.7 per cent of isolates, followed by allele 6 (10.3 per cent). Significant changes in allele prevalence were found between 2007, 2008 and 2010, with an increasing prevalence in SeM-9-related alleles and a corresponding decreasing prevalence in SeM-6-related alleles observed over the period (P<0.001). Geographical proximity of outbreaks caused by some uncommon alleles was apparent between 2007, 2008 and 2010.

Modular and Predictable Assembly of Porous Organic Molecular Crystals

Nature. Jun, 2011  |  Pubmed ID: 21677756

Nanoporous molecular frameworks are important in applications such as separation, storage and catalysis. Empirical rules exist for their assembly but it is still challenging to place and segregate functionality in three-dimensional porous solids in a predictable way. Indeed, recent studies of mixed crystalline frameworks suggest a preference for the statistical distribution of functionalities throughout the pores rather than, for example, the functional group localization found in the reactive sites of enzymes. This is a potential limitation for 'one-pot' chemical syntheses of porous frameworks from simple starting materials. An alternative strategy is to prepare porous solids from synthetically preorganized molecular pores. In principle, functional organic pore modules could be covalently prefabricated and then assembled to produce materials with specific properties. However, this vision of mix-and-match assembly is far from being realized, not least because of the challenge in reliably predicting three-dimensional structures for molecular crystals, which lack the strong directional bonding found in networks. Here we show that highly porous crystalline solids can be produced by mixing different organic cage modules that self-assemble by means of chiral recognition. The structures of the resulting materials can be predicted computationally, allowing in silico materials design strategies. The constituent pore modules are synthesized in high yields on gram scales in a one-step reaction. Assembly of the porous co-crystals is as simple as combining the modules in solution and removing the solvent. In some cases, the chiral recognition between modules can be exploited to produce porous organic nanoparticles. We show that the method is valid for four different cage modules and can in principle be generalized in a computationally predictable manner based on a lock-and-key assembly between modules.

Effects of Daily Activities on DXA Measurements of Body Composition in Active People

Medicine and Science in Sports and Exercise. Jun, 2011  |  Pubmed ID: 21685816

PURPOSE:: Dual energy x-ray absorptiometry (DXA) is becoming a popular tool to measure body composition in athletes, owing to its ease of operation and comprehensive analysis of body composition. This study represents the first systematic investigation of reliability of DXA measurements of body composition in trained individuals, and includes measurements of daily variability as well as the specific effect of the intake of a meal. METHODS:: Physically active young adults (15 females, 16 males) underwent five whole-body DXA scans over a 2 d period: in the morning after an overnight fast, ∼5 min later after repositioning on the scanning bed, ∼8 h later following usual daily activities, and the next morning before and ∼30 min after consumption of a simple breakfast. Magnitudes of typical (standard) errors of measurement and changes in the mean of DXA measures were assessed by standardization. RESULTS:: Repositioning produced trivial typical errors for whole body composition, whereas regional body composition showed substantial errors. Daily activities and consumption of breakfast generally produced substantial increase in the typical error and mean of DXA estimates of total and regional lean mass, and associated body mass. CONCLUSION:: Having a standardized scanning protocol and fasted subjects is the most practical way to minimize measurement errors. Future studies involving DXA in measuring body composition should report their scanning and analysis protocol with their associated typical errors of measurement so that the level of reliability can be assessed.

Maternal Exposure to Household Chemicals and Risk of Infant Leukemia: a Report from the Children's Oncology Group

Cancer Causes & Control : CCC. Aug, 2011  |  Pubmed ID: 21691732

Utilizing data from the largest study to date, we examined associations between maternal preconception/prenatal exposure to household chemicals and infant acute leukemia.

'Trying to Make It All Come Together': Structuration and Employed Mothers' Experience of Family Food Provisioning in Canada

Health Promotion International. Jun, 2011  |  Pubmed ID: 21693474

This research examined the aetiology of employed mothers' food choice and food provisioning decisions using a qualitative, grounded theory methodology. Semi-structured interviews using the Food Choice Map were conducted with eleven middle-income employed mothers of elementary school-age children. Results demonstrated that the women exhibited conflicting identities with respect to food choice and provisioning. As 'good mothers' they were the primary food and nutrition caregivers for the family, desiring to provide healthy, homemade foods their families preferred at shared family meals. They also sought to be independent selves, working outside the home, within the context of a busy modern family. Increased food autonomy of children, and lack of time due to working outside the home and children's involvement in extracurricular activities, were significant influences on their food choice and provisioning. This resulted in frequently being unable to live up to their expectations of consistently providing healthy homemade foods and having shared family meals. To cope, the women frequently relied on processed convenience and fast foods despite their acknowledged inferior nutritional status. Using Giddens' structuration theory, the dynamic relationships between the women's food choice and provisioning actions, their identities and larger structures including socio-cultural norms, conditions of work and the industrial food system were explored. The ensuing dietary pattern of the women and their families increases the risk of poor health outcomes, including obesity. These results have implications for public health responses to improve population health by shifting the focus from individual-level maternal influences to structural influences on diet.

Retention of Provided Identification for Dogs and Cats Seen in Veterinary Clinics and Adopted from Shelters in Oklahoma City, OK, USA

Preventive Veterinary Medicine. Sep, 2011  |  Pubmed ID: 21704398

Personalized identification (ID) tags that contain contact information for the dog or cat owner can help assure lost animals are quickly reunited with their owners. The authors have previously reported that while the majority of pet owners stated that ID tags were very important, only a third responded that their pet wears an ID tag. The objective of this study was to evaluate if providing and putting on the pet a free collar and ID tag during an owner's visit to a veterinary hospital or spay/neuter facility increased the likelihood that the pet owners would actually keep the identification on their pet at least 6-8 weeks after they were placed on the pet. A second population of dogs and cats that were adopted from animal control and humane society shelters were also studied to assess retention of a collar and personalized ID tag. Telephone follow-up occurred a mean of 8 weeks after the tag was applied. Retaining and using the tag significantly increased for the veterinary group with 13.8% reporting their pets were currently wearing an ID tag pre-intervention, and 84.3% reporting their pets were currently wearing and ID tag post-intervention. Of the dogs and cats that were adopted, at follow-up 94% of owners reported their pets were currently wearing an ID tag. Approximately 5% of those who participated in the post-intervention survey lost a pet and recovered that pet because of the ID tag. This suggests that ID tagging is an effective method to potentially decrease stray intake into shelters and return pets home. The data also support placing tags and collars directly on pets as a method to retain those ID tags and collars on the animals, thus increasing the likelihood they will be returned home if lost or during a disaster.

Effectiveness of a Mass Media Campaign in Promoting HIV Testing Information Seeking Among African American Women

Journal of Health Communication. Oct, 2011  |  Pubmed ID: 21707409

"Take Charge. Take the Test." (TCTT), a media campaign promoting HIV testing among African American women, was piloted in Cleveland and Philadelphia from October 2006 to October 2007. This study assesses TCTT's effectiveness in promoting HIV testing information seeking among target audiences in each pilot city. The authors analyzed data on telephone hotlines promoted by the campaign and the www.hivtest.org Web site to examine trends in hotline calls and testing location searches before, during, and after the campaign. Cleveland hotline data were available from October 1, 2005, through February 28, 2008, for a total of 29 months (N = 126 weeks). Philadelphia hotline data were available from May 1, 2006, through February 28, 2008, for a total of 22 months (N = 96 weeks). The authors assessed the relation between market-level measures of the campaign's advertising activities and trends in hotline call volume and testing location searches. They found a significant relation between measures of TCTT advertising and hotline calls. Specifically, they found that increases in advertising gross ratings points were associated with increases in call volume, controlling for caller demographics and geographic location. The campaign had similar effects on HIV testing location searches. Overall, it appears the campaign generated significant increases in HIV information seeking. Results are consistent with other studies that have evaluated the effects of media campaigns on similar forms of information seeking. This study illustrates useful methods for evaluating campaign effects on information seeking with data on media implementation, hotline calls, and zip code-based searches for testing locations.

Eomesodermin, HAND1, and CSH1 Proteins Are Induced by Cellular Stress in a Stress-activated Protein Kinase-dependent Manner

Molecular Reproduction and Development. Jul, 2011  |  Pubmed ID: 21710638

Eomesodermin (Eomes) is a transcription factor essential for trophoblast development. Stress stimuli activate stress-activated protein kinase (MAPK8/9) and modulate transcription factors in trophoblast stem cells (TSC). In this study, we test the hypothesis that stress-induced Eomes upregulation and downstream trophoblast development are MAPK8/9-dependent. Immunocytochemical and immunoblot assays suggest that Eomes is induced by hyperosmolar stress in a dose- and time-dependent manner. Two MAPK8/9 inhibitors that work by different mechanisms, LJNKl1 and SP600125, block induction of Eomes protein by stress. During normal TSC differentiation, the transcription factor heart and neural crest derivatives expressed 1 (HAND1) is dependent on Eomes, and chorionic somatomammotropin hormone 1 (CSH1) expression is dependent on HAND1. Similar to Eomes, HAND1 and CSH1 induction by stress are MAPK8/9-dependent, and CSH1 is induced in nearly all stressed TSC. CSH1 induction normally requires downregulation of the transcription factor inhibitor of differentiation 2 (ID2) as well as HAND1 upregulation. It was shown previously that hyperosmolar stress induces AMP-activated protein kinase (PRKAA1/2)-dependent ID2 loss in a MAPK8/9-independent manner. Inhibition of PRKAA1/2 with compound C and LJNKl1, more than MAPK8/9 inhibitors alone, inhibits the induction of CSH1 by stress. Taken together these data suggest that stress-induced MAPK8/9 and PRKAA1/2 regulate transcription factors Eomes/HAND1 and ID2, respectively. Together this network mediates induction of CSH1 by stress. Therefore, stress triggers a proportional increase in a normal early TSC differentiation event that could be adaptive in inducing CSH1. But the flexibility of TSC to undergo stress-induced differentiation could lead to pathophysiological consequences if stress endured and TSC differentiation became unbalanced.

Effect of Sodium Bicarbonate on [HCO3-], PH, and Gastrointestinal Symptoms

International Journal of Sport Nutrition and Exercise Metabolism. Jun, 2011  |  Pubmed ID: 21719899

Sodium bicarbonate (NaHCO₃) is often ingested at a dose of 0.3 g/kg body mass (BM), but ingestion protocols are inconsistent in terms of using solution or capsules, ingestion period, combining NaHCO₃ with sodium citrate (Na₃C₆H₅O₇), and coingested food and fluid.

FMR1 Intron 1 Methylation Predicts FMRP Expression in Blood of Female Carriers of Expanded FMR1 Alleles

The Journal of Molecular Diagnostics : JMD. Sep, 2011  |  Pubmed ID: 21723415

Fragile X syndrome (FXS) is caused by loss of the fragile X mental retardation gene protein product (FMRP) through promoter hypermethylation, which is usually associated with CGG expansion to full mutation size (>200 CGG repeats). Methylation-sensitive Southern blotting is the current gold standard for the molecular diagnosis of FXS. For females, Southern blotting provides the activation ratio (AR), which is the proportion of unmethylated alleles on the active X chromosome. Herein, we examine the relationship of FMRP expression with methylation patterns of two fragile X-related epigenetic elements (FREE) analyzed using matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry and the AR. We showed that the differential methylation of the FREE2 sequence within fragile X mental retardation gene intron 1 was related to depletion of FMRP expression. We also show that, using the combined cohort of 12 females with premutation (55 to 200 CGG repeats) and 22 females with full mutation alleles, FREE2 methylation analysis was superior to the AR as a predictor of the proportion of FMRP-positive cells in blood. Because matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry is amenable to high-throughput processing and requires minimal DNA, these findings have implications for routine FXS testing and population screening.

Partial Breast Irradiation Delivered with Proton Beam: Results of a Phase II Trial

Clinical Breast Cancer. Aug, 2011  |  Pubmed ID: 21729673

A phase II trial sought to determine the safety and efficacy of proton beam irradiation to deliver partial breast radiotherapy after lumpectomy for early-stage breast cancer.

An in Vitro Model of the Glomerular Capillary Wall Using Electrospun Collagen Nanofibres in a Bioartificial Composite Basement Membrane

PloS One. 2011  |  Pubmed ID: 21731625

The filtering unit of the kidney, the glomerulus, contains capillaries whose walls function as a biological sieve, the glomerular filtration barrier. This comprises layers of two specialised cells, glomerular endothelial cells (GEnC) and podocytes, separated by a basement membrane. Glomerular filtration barrier function, and dysfunction in disease, remains incompletely understood, partly due to difficulties in studying the relevant cell types in vitro. We have addressed this by generation of unique conditionally immortalised human GEnC and podocytes. However, because the glomerular filtration barrier functions as a whole, it is necessary to develop three dimensional co-culture models to maximise the benefit of the availability of these cells. Here we have developed the first two tri-layer models of the glomerular capillary wall. The first is based on tissue culture inserts and provides evidence of cell-cell interaction via soluble mediators. In the second model the synthetic support of the tissue culture insert is replaced with a novel composite bioartificial membrane. This consists of a nanofibre membrane containing collagen I, electrospun directly onto a micro-photoelectroformed fine nickel supporting mesh. GEnC and podocytes grew in monolayers on either side of the insert support or the novel membrane to form a tri-layer model recapitulating the human glomerular capillary in vitro. These models will advance the study of both the physiology of normal glomerular filtration and of its disruption in glomerular disease.

Validity and Reliability of Perinatal Biomarkers of Adiposity After Storage As Dried Blood Spots on Paper

American Journal of Human Biology : the Official Journal of the Human Biology Council. Sep-Oct, 2011  |  Pubmed ID: 21735507

To validate use of chip-based immunoaffinity capillary electrophoresis on dried blood spot samples (DBSS) to measure obesity-related hormones.

'Trails B or Not Trails B?' Is Attention-switching a Useful Outcome Measure?

Brain Injury : [BI]. 2011  |  Pubmed ID: 21745176

Difficulties with attention contribute to behavioural and cognitive problems during childhood and may reflect subtle deficits in executive functioning (EF). Attention problems in early childhood have also been found to predict higher levels of anxiety and depression symptoms at 10 years old. It has also been reported that attention problems during childhood may be differentially related to later-emerging distinct EF difficulties. Many of these findings, however, rely on teacher-ratings of attention difficulties.

Comparison Between Conventional and Piezoelectric Surgical Tools for Maxillary Sinus Floor Elevation. A Randomized Controlled Clinical Trial

Clinical Implant Dentistry and Related Research. Jul, 2011  |  Pubmed ID: 21745325

Aim: The aim of this study was to assess the performance of conventional rotative instruments and a piezoelectric device for maxillary sinus floor elevation surgery, and to assess whether application of a resorbable membrane reduces resorption of an augmented site in a randomized clinical trial. Materials and Methods: Thirty-six consecutive patients (59.2 ± 10.7 years, range 38-76 years) needing bilateral sinus floor elevation surgery agreed to participate in this study. In a parallel split mouth design randomized clinical trial, in which the allocation of the surgical technique to be used on the determined sites was randomly assigned, one site was always treated with conventional rotative instruments (control group) and the other site with piezosurgery (test group). In addition, in a random order, the grafted sites were covered with a collagen membrane or no membrane. After a healing period of 3-4 months implants were placed. Results: Comparison of clinical features of the test and control sites revealed no differences with regard to wound healing and complications (perforations of the sinus membrane) during or postsurgery (p = .458, p = 1.0, respectively). A clinically insignificant, but statistically shorter operation time was observed when using conventional rotative instruments (11.1 ± 2.4 minutes) than using piezosurgery (15.1 ± 2.9 minutes; p < .001). In both groups, application of a resorbable membrane did not result in less horizontal bone resorption (membrane: 1.43 mm, no membrane: 1.06 mm; p = .062); All 193 implants could be placed with primary stability. One year after functional loading, survival rate was 100%. Conclusion: It can be concluded that, for maxillary sinus floor elevation surgery, a piezoelectric device shows no advantages over rotative instruments as well as that placement of a barrier membrane did not reduce resorption of the augmented site.

Search for a Heavy Particle Decaying into an Electron and a Muon with the ATLAS Detector in Sqrt[s] = 7 TeV Pp Collisions at the LHC

Physical Review Letters. Jun, 2011  |  Pubmed ID: 21770629

This Letter presents the first search for a heavy particle decaying into an e ± μ(-/+) final state in sqrt[s] = 7 TeV pp collisions at the LHC. The data were recorded by the ATLAS detector during 2010 and correspond to a total integrated luminosity of 35 pb(-1). No excess above the standard model background expectation is observed. Exclusions at 95% confidence level are placed on two representative models. In an R-parity violating supersymmetric model, tau sneutrinos with a mass below 0.75 TeV are excluded, assuming all R-parity violating couplings are zero except λ(311)' = 0.11 and λ312 = 0.07. In a lepton flavor violating model, a Z'-like vector boson with masses of 0.70-1.00 TeV and corresponding cross sections times branching ratios of 0.175-0.183 pb is excluded. These results extend to higher mass R-parity violating sneutrinos and lepton flavor violating Z's than previous constraints from the Tevatron.

Acute Laminar Shear Stress Reversibly Increases Human Glomerular Endothelial Cell Permeability Via Activation of Endothelial Nitric Oxide Synthase

American Journal of Physiology. Renal Physiology. Oct, 2011  |  Pubmed ID: 21775480

Laminar shear stress is a key determinant of systemic vascular behavior, including through activation of endothelial nitric oxide synthase (eNOS), but little is known of its role in the glomerulus. We confirmed eNOS expression by glomerular endothelial cells (GEnC) in tissue sections and examined effects of acute exposure (up to 24 h) to physiologically relevant levels of laminar shear stress (10-20 dyn/cm(2)) in conditionally immortalized human GEnC. Laminar shear stress caused an orientation of GEnC and stress fibers parallel to the direction of flow and induced Akt and eNOS phosphorylation along with NO production. Inhibition of the phophatidylinositol (PI)3-kinase/Akt pathway attenuated laminar shear stress-induced eNOS phosphorylation and NO production. Laminar shear stress of 10 dyn/cm(2) had a dramatic effect on GEnC permeability, reversibly decreasing the electrical resistance across GEnC monolayers. Finally, the laminar shear stress-induced reduction in electrical resistance was attenuated by the NOS inhibitors l-N(G)-monomethyl arginine (l-NMMA) and l-N(G)-nitroarginine methyl ester (l-NAME) and also by inhibition of the PI3-kinase/Akt pathway. Hence we have shown for GEnC in vitro that acute permeability responses to laminar shear stress are dependent on NO, produced via activation of the PI3-kinase/Akt pathway and increased eNOS phosphorylation. These results suggest the importance of laminar shear stress and NO in regulating the contribution of GEnC to the permeability properties of the glomerular capillary wall.

Size-exclusion Chromatography of Perfluorosulfonated Ionomers

Journal of Chromatography. A. Aug, 2011  |  Pubmed ID: 21782181

A size-exclusion chromatography (SEC) method in N,N-dimethylformamide containing 0.1 M LiNO(3) is shown to be suitable for the determination of molar mass distributions of three classes of perfluorosulfonated ionomers, including Nafion(®). Autoclaving sample preparation is optimized to prepare molecular solutions free of aggregates, and a solvent exchange method concentrates the autoclaved samples to enable the use of molar-mass-sensitive detection. Calibration curves obtained from light scattering and viscometry detection suggest minor variation in the specific refractive index increment across the molecular size distributions, which introduces inaccuracies in the calculation of local absolute molar masses and intrinsic viscosities. Conformation plots that combine apparent molar masses from light scattering detection with apparent intrinsic viscosities from viscometry detection partially compensate for the variations in refractive index increment. The conformation plots are consistent with compact polymer conformations, and they provide Mark-Houwink-Sakurada constants that can be used to calculate molar mass distributions without molar-mass-sensitive detection. Unperturbed dimensions and characteristic ratios calculated from viscosity-molar mass relationships indicate unusually free rotation of the perfluoroalkane backbones and may suggest limitations to applying two-parameter excluded volume theories for these ionomers.

The Missing Piece: a Sociological Autopsy of Firearm Suicide in the United States

Suicide & Life-threatening Behavior. Oct, 2011  |  Pubmed ID: 21793873

Social, economic, violence, political, and gun access predictors of suicide and gun suicide were examined via sociological autopsy. The model predicting suicide rates overall had the best results, χ(2) (9, N=50)=5.279 (CMIN, the goodness of fit statistic that represents the minimum discrepancy between the unrestricted sample covariance matrix and the restricted covariance matrix) p=.809, [the goodness of fit statistic that represents the minimum discrepancy between the unrestricted sample covariance matrix and the restricted covariance matrix] indicating an excellent fit of the data and theoretical model. The model explained 76% of the variance in state suicide rates and was a significantly better predictor than one could expect by chance, F(6,43)=22.889, p<.001). All path coefficients were significant predictors of suicide with the exception of violence climate, which was not included. This study contributes to the theoretical knowledge by adding a comprehensive framework of analysis and model useful for prevention.

Relationships Between Early Acute Pain Scores, Autonomic Nervous System Function, and Injury Severity in Wounded Soldiers

The Journal of Trauma. Jul, 2011  |  Pubmed ID: 21795892

Acute pain after injury affects the comfort and function of the wounded soldier and the physiology of multiple body systems. In the civilian population, pain alters the function of the autonomic nervous system, causing increased heart rate and blood pressure. However, there are no data regarding the impact of combat-related pain on physiologic responses. This study is a retrospective analysis that examined the relationship of pain and physiologic parameters in injured soldiers.

GRP78(BiP) Facilitates the Cytosolic Delivery of Anthrax Lethal Factor (LF) in Vivo and Functions As an Unfoldase in Vitro

Molecular Microbiology. Sep, 2011  |  Pubmed ID: 21797942

Anthrax toxin is an A/B bacterial protein toxin which is composed of the enzymatically active Lethal Factor (LF) and/or Oedema Factor (EF) bound to Protective Antigen 63 (PA63) which functions as both the receptor binding and transmembrane domains. Once the toxin binds to its cell surface receptors it is internalized into the cell and traffics through Rab5- and Rab7-associated endosomal vesicles. Following acidification of the vesicle lumen, PA63 undergoes a dynamic change forming a beta-barrel that inserts into and forms a pore through the endosomal membrane. It is widely recognized that LF, and the related fusion protein LFnDTA, must be completely denatured in order to transit through the PA63 formed pore and enter the eukaryotic cell cytosol. We demonstrate by protease protection assays that the molecular chaperone GRP78 mediates the unfolding of LFnDTA and LF at neutral pH and thereby converts these proteins from a trypsin resistant to sensitive conformation. We have used immunoelectron microscopy and gold-labelled antibodies to demonstrate that both GRP78 and GRP94 chaperones are present in the lumen of endosomal vesicles. Finally, we have used siRNA to demonstrate that knock-down of GRP78 results in the emergence of resistance to anthrax lethal toxin and oedema toxin action.

Comments on Cow's Milk Allergy and Diphtheria, Tetanus, and Pertussis Vaccines

The Journal of Allergy and Clinical Immunology. Aug, 2011  |  Pubmed ID: 21807255

Whistleblower Protection

Ohio Nurses Review. Mar-Apr, 2011  |  Pubmed ID: 21815328

Comparison of Commercial DNA Extraction Kits for Isolation and Purification of Bacterial and Eukaryotic DNA from PAH-contaminated Soils

Canadian Journal of Microbiology. Aug, 2011  |  Pubmed ID: 21815819

Molecular characterization of the microbial populations of soils and sediments contaminated with polycyclic aromatic hydrocarbons (PAHs) is often a first step in assessing intrinsic biodegradation potential. However, soils are problematic for molecular analysis owing to the presence of organic matter, such as humic acids. Furthermore, the presence of contaminants, such as PAHs, can cause further challenges to DNA extraction, quantification, and amplification. The goal of our study was to compare the effectiveness of four commercial soil DNA extraction kits (UltraClean Soil DNA Isolation kit, PowerSoil DNA Isolation kit, PowerMax Soil DNA Isolation kit, and FastDNA SPIN kit) to extract pure, high-quality bacterial and eukaryotic DNA from PAH-contaminated soils. Six different contaminated soils were used to determine if there were any biases among the kits due to soil properties or level of contamination. Extracted DNA was used as a template for bacterial 16S rDNA and eukaryotic 18S rDNA amplifications, and PCR products were subsequently analyzed using denaturing gel gradient electrophoresis (DGGE). We found that the FastDNA SPIN kit provided significantly higher DNA yields for all soils; however, it also resulted in the highest levels of humic acid contamination. Soil texture and organic carbon content of the soil did not affect the DNA yield of any kit. Moreover, a liquid-liquid extraction of the DNA extracts found no residual PAHs, indicating that all kits were effective at removing contaminants in the extraction process. Although the PowerSoil DNA Isolation kit gave relatively low DNA yields, it provided the highest quality DNA based on successful amplification of both bacterial and eukaryotic DNA for all six soils. DGGE fingerprints among the kits were dramatically different for both bacterial and eukaryotic DNA. The PowerSoil DNA Isolation kit revealed multiple bands for each soil and provided the most consistent DGGE profiles among replicates for both bacterial and eukaryotic DNA.

Single-Tooth Implants with Different Neck Designs: A Randomized Clinical Trial Evaluating the Aesthetic Outcome

Clinical Implant Dentistry and Related Research. Aug, 2011  |  Pubmed ID: 21815991

Aim: To evaluate the aesthetic outcome of single-tooth implants in the aesthetic zone with different neck designs from a professional's and patient's perception. Materials and Methods: Ninety-three patients with a missing anterior tooth in the maxilla were randomly assigned to be treated with an implant with a smooth neck, a rough neck with grooves or a scalloped rough neck with grooves. Implants were installed in healed sites. One year after definitive crown placement (18 months post-implant placement), photographs were taken and the aesthetic outcome was assessed according to two objective aesthetic indexes: pink esthetic score/white esthetic score (PES/WES) and implant crown aesthetic index (ICAI). A questionnaire was used to assess the aesthetic outcome and general satisfaction from a patient's perception. Standardized radiographs were taken to measure marginal bone level changes. Results: One implant was lost. Although there was a significant difference in marginal bone loss between the different implant neck designs (smooth neck 1.19 ± 0.82 mm, rough neck 0.90 ± 0.57 mm, scalloped neck 2.01 ± 0.77 mm), there were no differences in aesthetic outcome. According to the professional's assessments using PES/WES and ICAI, 79.3% and 62% of the cases showed acceptable crown aesthetics, and 59.8% and 56.5% of the cases showed acceptable mucosa aesthetics. Overall, patients were satisfied about the aesthetics of the mucosa (81.5%) and crown (93.3%), and general patient satisfaction was high (9.0 ± 1.0 out of a maximum of 10). According to the professional's assessment, a pre-implant augmentation procedure was associated with less favorable aesthetics of the mucosa. Conclusion: This study shows that the aesthetics of single-tooth implants in the maxillary aesthetic zone appears to be independent of the implant neck designs applied but dependent on the need for pre-implant surgery.

Expression of Transposable Elements in Neural Tissues During Xenopus Development

PloS One. 2011  |  Pubmed ID: 21818339

Transposable elements comprise a large proportion of animal genomes. Transposons can have detrimental effects on genome stability but also offer positive roles for genome evolution and gene expression regulation. Proper balance of the positive and deleterious effects of transposons is crucial for cell homeostasis and requires a mechanism that tightly regulates their expression. Herein we describe the expression of DNA transposons of the Tc1/mariner superfamily during Xenopus development. Sense and antisense transcripts containing complete Tc1-2_Xt were detected in Xenopus embryos. Both transcripts were found in zygotic stages and were mainly localized in Spemann's organizer and neural tissues. In addition, the Tc1-like elements Eagle, Froggy, Jumpy, Maya, Xeminos and TXr were also expressed in zygotic stages but not oocytes in X. tropicalis. Interestingly, although Tc1-2_Xt transcripts were not detected in Xenopus laevis embryos, transcripts from other two Tc1-like elements (TXr and TXz) presented a similar temporal and spatial pattern during X. laevis development. Deep sequencing analysis of Xenopus tropicalis gastrulae showed that PIWI-interacting RNAs (piRNAs) are specifically derived from several Tc1-like elements. The localized expression of Tc1-like elements in neural tissues suggests that they could play a role during the development of the Xenopus nervous system.

Illusory Contour Figures Are Perceived As Occluding Contours by 4-month-old Infants

Developmental Psychology. Aug, 2011  |  Pubmed ID: 21823790

Although 4-month-olds perceive continuity of an object's trajectory through occlusion, little is known about the information specifying an occluding surface at this age. We investigated this in 3 experiments involving 84 participants. Testing the claim that 5-month-olds are unable to perceive the Kanizsa figure as an occluding surface (Csibra, 2001), we demonstrated that 4-month-olds perceived trajectory continuity behind this figure providing its horizontal extent was small. We demonstrated that the presence of visible occluding edges or occlusion of background was insufficient to specify an occluding surface but that their combination was sufficient. Thus, beyond object deletion and accretion, both visible occluding edges and occlusion of background are necessary for perception of occluding surfaces at this age. (PsycINFO Database Record (c) 2011 APA, all rights reserved).

Question Whether Dog Bites Are on the Rise

Journal of the American Veterinary Medical Association. Mar, 2011  |  Pubmed ID: 21452592

Abundant Non-canonical DUTP Found in Primary Human Macrophages Drives Its Frequent Incorporation by HIV-1 Reverse Transcriptase

The Journal of Biological Chemistry. Jul, 2011  |  Pubmed ID: 21454906

Terminally differentiated/non-dividing macrophages contain extremely low cellular dNTP concentrations (20-40 nm), compared with activated CD4(+) T cells (2-5 μm). However, our LC-MS/MS study revealed that the non-canonical dUTP concentration (2.9 μm) is ∼60 times higher than TTP in macrophages, whereas the concentrations of dUTP and TTP in dividing human primary lymphocytes are very similar. Specifically, we evaluated the contribution of HIV-1 reverse transcriptase to proviral DNA uracilation under the physiological conditions found in HIV-1 target cells. Indeed, biochemical simulation of HIV-1 reverse transcription demonstrates that HIV-1 RT efficiently incorporates dUTP in the macrophage nucleotide pools but not in the T cell nucleotide pools. Measurement of both pre-steady state and steady state kinetic parameters of dUTP incorporation reveals minimal selectivity of HIV-1 RT for TTP over dUTP, implying that the cellular dUTP/TTP ratio determines the frequency of HIV-1 RT-mediated dUTP incorporation. The RT of another lentivirus, simian immunodeficiency virus, also displays efficient dUTP incorporation in the dNTP/dUTP pools found in macrophages but not in T cells. Finally, 2',3'-dideoxyuridine was inhibitory to HIV-1 proviral DNA synthesis in macrophages but not in T cells. The data presented demonstrates that the non-canonical dUTP was abundant relative to TTP, and efficiently incorporated during HIV-1 reverse transcription, particularly in non-dividing macrophages.

Thematic Analysis™: a Chemogenomic Approach to GPCR Drug Discovery

Current Topics in Medicinal Chemistry. 2011  |  Pubmed ID: 21470170

Thematic Analysis™ is a chemogenomic tool which has been developed and used to aid the process of GPCR drug discovery. This review covers the scientific rationale behind the development of this tool and provides examples of the successful application of the chemogenomic method in both hit finding and hit to lead stages of the drug discovery process.

Multifocal Oral Langerhans Cell Histiocytosis

Journal of Oral and Maxillofacial Surgery : Official Journal of the American Association of Oral and Maxillofacial Surgeons. Oct, 2011  |  Pubmed ID: 21470739

Preventing Infection with Long-term Indwelling Urinary Catheters

British Journal of Community Nursing. Apr, 2011  |  Pubmed ID: 21471917

This article sets out to inform readers on the care and management of indwelling catheters. The reason for urinary catheter insertion is discussed and the decision to use a long-term indwelling urinary catheter is explained. This article focuses on the risk of urinary tract infections (UTI) associated with the use of long-term indwelling catheters and how these may be avoided. Examples of current products are detailed and how the selection of the right product may be achieved is also outlined.

Analysis of White Blood Cell Counts in Mice After Gamma- or Proton-radiation Exposure

Radiation Research. Aug, 2011  |  Pubmed ID: 21476859

In the coming decades human space exploration is expected to move beyond low-Earth orbit. This transition involves increasing mission time and therefore an increased risk of radiation exposure from solar particle event (SPE) radiation. Acute radiation effects after exposure to SPE radiation are of prime importance due to potential mission-threatening consequences. The major objective of this study was to characterize the dose-response relationship for proton and γ radiation delivered at doses up to 2 Gy at high (0.5 Gy/min) and low (0.5 Gy/h) dose rates using white blood cell (WBC) counts as a biological end point. The results demonstrate a dose-dependent decrease in WBC counts in mice exposed to high- and low-dose-rate proton and γ radiation, suggesting that astronauts exposed to SPE-like radiation may experience a significant decrease in circulating leukocytes.

Synthesis of Silicon Doped SrMO3 (M = Mn, Co): Stabilization of the Cubic Perovskite and Enhancement in Conductivity

Dalton Transactions (Cambridge, England : 2003). May, 2011  |  Pubmed ID: 21491046

In this paper we report the successful incorporation of silicon into SrMO(3) (M = Co, Mn) leading to a structural change from a hexagonal to a cubic perovskite. For M = Co, the cubic phase was observed for low doping levels (3%), and these doped phases showed very high conductivities (up to ≈350 Scm(-1) at room temperature). However, annealing studies at intermediate temperatures (700-800 °C), indicated that the cubic phase was metastable with a gradual transformation to a hexagonal cell on annealing. Further work showed that co-doping with Fe resulted in improved stability of the cubic phase; a composition SrCo(0.85)Fe(0.1)Si(0.05)O(3-y) displayed good stability at intermediate temperatures and a high conductivity (≈150 Scm(-1) at room temperature). For M = Mn, the work showed that higher substitution levels were required to form the cubic perovskite (≈15% Si doping), although in these cases the phases were shown to be stable to annealing at intermediate temperatures. Conductivity measurements again showed an enhancement in the conductivity on Si doping, although the conductivities were lower (≈0.3-14 Scm(-1) in the range 20-800 °C) than the cobalt containing systems. The conductivities of both systems suggest potential for use as cathode materials in solid oxide fuel cells.

Molecular Characterisation of 'strangles' Outbreaks in the UK: the Use of M-protein Typing of Streptococcus Equi Ssp. Equi

Equine Veterinary Journal. May, 2011  |  Pubmed ID: 21492215

Strangles is the most commonly diagnosed and important infectious disease of horses worldwide. Very little is known about the temporo-spatial and molecular epidemiology of strangles. The disease is not notifiable in the UK and there are few published data on the geographical locations of outbreaks.

Family Support Groups: an Essential Contribution to Follow-up Care

Journal of Pediatric Gastroenterology and Nutrition. May, 2011  |  Pubmed ID: 21499047

Sequentially-induced Responses Define Tumour Cell Radiosensitivity

International Journal of Radiation Biology. Jun, 2011  |  Pubmed ID: 21501033

Our aim was to define dose-dependent and genotype-dependent components of radiosensitivity by resolving patterns of radiation-induced clonal inactivation into specific responses.

Disseminated Candidiasis Caused by Candida Albicans with Amino Acid Substitutions in Fks1 at Position Ser645 Cannot Be Successfully Treated with Micafungin

Antimicrobial Agents and Chemotherapy. Jul, 2011  |  Pubmed ID: 21502627

The clinical utility of the echinocandins is potentially compromised by the emergence of drug resistance. We investigated whether Candida albicans with amino acid substitutions at position Ser645 in Fks1 can be treated with either a conventional or an elevated dosage of micafungin. We studied Candida albicans (wild-type SC5314; MIC, 0.06 mg/liter) and four fks1 mutants (one FKS1/fks1 heterozygote mutant [MIC, 0.5 mg/liter] and three fks1/fks1 homozygous mutants [MICs for all, 2 mg/liter]) with a variety of amino acid substitutions at Ser645. The pharmacokinetic and pharmacodynamic relationships were characterized in a persistently neutropenic murine model of disseminated candidiasis. A mathematical model was fitted to all pharmacokinetic and pharmacodynamic data. This mathematical model was then used to "humanize" the murine pharmacokinetics, and the predicted antifungal effect was determined. The estimated maximal rate of growth and ultimate fungal densities in the kidney for each of the strains were similar. The administration of micafungin at 1 mg/kg of body weight to the wild type resulted in moderate antifungal activity, whereas the administration of 5 and 20 mg/kg resulted in rapid fungicidal activity. In contrast, the FKS1/fks heterozygote was killed only with 20 mg/kg, and the homozygous fks1 mutants failed to respond to any dosage. The bridging study revealed that human dosages of 100 and 400 mg/day were active only against the wild type, with no activity against either the heterozygote or the homozygote mutants. Ser645 Fks1 Candida albicans mutants cannot be treated with either conventional or elevated dosages of micafungin and should be deemed resistant.

An Incremental Mean First Passage Analysis for a Quasistatic Model of Polymer Translocation Through a Nanopore

The Journal of Chemical Physics. Apr, 2011  |  Pubmed ID: 21513416

For the translocation of a polymer through a nanopore, a quasistatic assumption for the dynamics yields a tractable form for the entropic barrier. Although this is a much simplified model, interesting features such as robust scaling emerge from its application. To explore these details, we present a method of mapping the translocation process as an incremental mean first passage problem. In this approach, the quantity of interest is the average first time t(0) at which the polymer achieves a displacement of Δs in the translocation coordinate s. Constructing scenarios with different initial conditions and boundary conditions, analytic and exact numerical approaches are used to resolve the dynamics of translocation in detail and generate new insight into the nature of the entropic barrier.

Search for Diphoton Events with Large Missing Transverse Energy in 7 TeV Proton-proton Collisions with the ATLAS Detector

Physical Review Letters. Mar, 2011  |  Pubmed ID: 21517301

A search for diphoton events with large missing transverse energy is presented. The data were collected with the ATLAS detector in proton-proton collisions at √s=7 TeV at the CERN Large Hadron Collider and correspond to an integrated luminosity of 3.1 pb⁻¹. No excess of such events is observed above the standard model background prediction. In the context of a specific model with one universal extra dimension with compactification radius R and gravity-induced decays, values of 1/R<729 GeV are excluded at 95% C. L., providing the most sensitive limit on this model to date.

Search for Supersymmetry Using Final States with One Lepton, Jets, and Missing Transverse Momentum with the ATLAS Detector in ˆ�s=7 TeV Pp Collisions

Physical Review Letters. Apr, 2011  |  Pubmed ID: 21517374

This Letter presents the first search for supersymmetry in final states containing one isolated electron or muon, jets, and missing transverse momentum from √s=7  TeV proton-proton collisions at the LHC. The data were recorded by the ATLAS experiment during 2010 and correspond to a total integrated luminosity of 35  pb(-1). No excess above the standard model background expectation is observed. Limits are set on the parameters of the minimal supergravity framework, extending previous limits. Within this framework, for A(0)=0 GeV, tanβ=3, and μ>0 and for equal squark and gluino masses, gluino masses below 700 GeV are excluded at 95% confidence level.

Dermatological Applications of Gene Silencing RNA Technology

The British Journal of Dermatology. May, 2011  |  Pubmed ID: 21518325

The Design and Application of Target-focused Compound Libraries

Combinatorial Chemistry & High Throughput Screening. Jul, 2011  |  Pubmed ID: 21521154

Target-focused compound libraries are collections of compounds which are designed to interact with an individual protein target or, frequently, a family of related targets (such as kinases, voltage-gated ion channels, serine/cysteine proteases). They are used for screening against therapeutic targets in order to find hit compounds that might be further developed into drugs. The design of such libraries generally utilizes structural information about the target or family of interest. In the absence of such structural information, a chemogenomic model that incorporates sequence and mutagenesis data to predict the properties of the binding site can be employed. A third option, usually pursued when no structural data are available, utilizes knowledge of the ligands of the target from which focused libraries can be developed via scaffold hopping. Consequently, the methods used for the design of target-focused libraries vary according to the quantity and quality of structural or ligand data that is available for each target family. This article describes examples of each of these design approaches and illustrates them with case studies, which highlight some of the issues and successes observed when screening target-focused libraries.

Stimuli Responsive Poly(1-[11-acryloylundecyl]-3-methyl-imidazolium Bromide): Dewetting and Nanoparticle Condensation Phenomena

Langmuir : the ACS Journal of Surfaces and Colloids. Jun, 2011  |  Pubmed ID: 21526809

A stimuli-responsive homopolymer poly(ILBr) is fabricated via a "two-phase" atom transfer radical polymerization (ATRP) process, where ILBr stands for the reactive ionic liquid surfactant, 1-[11-acryloylundecyl]-3-methyl-imidazolium bromide. An extraordinarily wide molecular weight distribution (PDI = 6.0) was obtained by introducing the initiator (4-bromomethyl methyl benzoate) in a heterogeneous two-phase process. The molecular weight distribution of poly(ILBr) was characterized by size-exclusion chromatography (SEC). The resulting homopolymer was found to be surface active and stimuli responsive. Poly(ILBr) films coated on quartz exhibit stimuli-responsive dewetting after ion exchange of Br(-) by PF(6)(-). This dewetting phenomenon can be understood in chain segmental terms as a stimuli-induced structural relaxation and appears to be the first such reported stimuli-responsive polymeric dewetting. Titrating aqueous poly(ILBr) with aqueous bis(2-ethylhexyl)sulfosuccinate induces nanophase separation and results in the condensation of nanoparticles 30-60 nm in diameter.

Thoracic Aortic Mobile Thrombus: is There a Role for Early Surgical Intervention?

The Annals of Thoracic Surgery. Jun, 2011  |  Pubmed ID: 21529769

The diagnosis of thoracic aortic mobile thrombus (TAMT) is rare and is usually made after debilitating embolic events. The optimal treatment strategy is unknown. We report 14 patients with TAMT and aim to better define the role of early (less than 2 weeks) surgical thrombectomy.

Influence of Intracellular Trehalose Concentration and Pre-freeze Cell Volume on the Cryosurvival of Rapidly Frozen Human Erythrocytes

Cryobiology. Aug, 2011  |  Pubmed ID: 21530502

Significant interest exists in the application of trehalose, which has low permeability to the phospholipid bilayer, as a non-toxic intracellular cryopreservative for mammalian cells. Introduction of between 8 ± 3mM and 266 ± 22 mM trehalose into human erythrocytes using the membrane permeabilizing polymer PP-50 allowed investigation of the relationship between intracellular trehalose concentration, pre-freeze cell volume, and cryosurvival. Cellular cryosurvival increased approximately linearly with pre-freeze cell volume up to the normal volume of fresh cells; diminished cell survival correlated with subnormal pre-freeze cell volume in some cases even at >100mM intracellular trehalose concentration. Uptake of >200 mM trehalose in cells with near-normal cell volume facilitated enhancement of cellular cryosurvival by up to 15 ± 5%.

Long-term Oral Appliance Therapy in Obstructive Sleep Apnea Syndrome: a Controlled Study on Temporomandibular Side Effects

Clinical Oral Investigations. May, 2011  |  Pubmed ID: 21538074

The objective of this study was to assess variations in the occurrence of temporomandibular disorders (TMDs) and the risk of developing pain and function impairment of the temporomandibular complex in obstructive sleep apnea syndrome (OSAS) patients treated with either an oral appliance (mandibular advancement device) or continuous positive airway pressure (CPAP) in a 2-year follow-up study. In addition, we assessed the relationship between the mean mandibular protrusion and the frequency of wearing the appliance during follow-up with the occurrence of pain and function impairment of the temporomandibular complex. Fifty-one patients were randomized to oral appliance therapy and 52 patients to CPAP therapy. TMDs (diagnosed according to the Axis I Research Diagnostic Criteria for TMD), pain intensity and disability and mandibular function impairment were recorded at baseline, after 2 months, 1 year and 2 years of therapy. Only in the initial period of treatment the occurrence of pain-related TMDs was considerably higher (24%) in the oral appliance group compared to CPAP (6%). Oral appliance therapy furthermore resulted in more temporomandibular pain compared to CPAP (odds ratio 2.33, 95% confidence interval (1.22-4.43)). However, there were no limitations in mandibular function in both groups during the (entire) follow-up period. Although generally not serious and of transient nature, oral appliance therapy results in more pain-related TMDs in the initial period of use compared with CPAP therapy. Oral appliance therapy is associated with increased pain in the temporomandibular complex in the initial period of use. Because of the transient nature, this pain is not a reason to contra-indicate an oral appliance in OSAS patients. Moreover, TMDs and the risk of developing pain and function impairment of the temporomandibular complex appear limited with long-term oral appliance use.

Two- to Eight-month-old Infants' Perception of Dynamic Auditory-visual Spatial Colocation

Child Development. Jul-Aug, 2011  |  Pubmed ID: 21545580

From birth, infants detect associations between the locations of static visual objects and sounds they emit, but there is limited evidence regarding their sensitivity to the dynamic equivalent when a sound-emitting object moves. In 4 experiments involving thirty-six 2-month-olds, forty-eight 5-month-olds, and forty-eight 8-month-olds, we investigated infants' ability to process this form of spatial colocation. Whereas there was no evidence of spontaneous sensitivity, all age groups detected a dynamic colocation during habituation and looked longer at test trials in which sound and sight were dislocated. Only 2-month-olds showed clear sensitivity to the dislocation relation, although 8-month-olds did so following additional habituation. These results are discussed relative to the intersensory redundancy hypothesis and work suggesting increasing specificity in processing with age.

Outcomes in Unresectable and Locally Advanced Resected Cholangiocarcinoma

Expert Review of Anticancer Therapy. May, 2011  |  Pubmed ID: 21554045

Patients with cholangiocarcinomas often present with unresectable disease, which is associated with a poor clinical outcome and survival. A number of palliative options are available to patients; the evaluated article presented experience from a single institution of treating cholangiocarcinoma, either unresectable or locally advanced, with conformal radiotherapy and concurrent chemotherapy. Patients who had received biliary radiation for cholangiocarcinoma were identified from the hospital database, and information on the patients sourced from notes and reports. In total, 20 patients with a diagnosis of biliary tract cancer were included and received radical conformal radiotherapy with concurrent cisplatin/5-fluorouracil and sequential gemcitabine. The median overall survival was 20.4 months and the relapse-free survival was 9.6 months. Treatment failure within the radiotherapy field was recorded in 45% of patients; adverse events were minimal. This study adds to the retrospective data available regarding the management of patients with biliary tract carcinomas, and we have found in our own cohort of 45 patients that gemcitabine/platinum was a more effective combination than monotherapy.

Predictors of Cognition in Adults with HIV: Implications for Nursing Practice and Research

The Journal of Neuroscience Nursing : Journal of the American Association of Neuroscience Nurses. Feb, 2011  |  Pubmed ID: 21338043

The objective of this study was to identify predictors of cognitive performance among adults with HIV. Participants completed demographic, psychosocial, and mental and physical health questionnaires as well as cognitive measures of speed of processing, psychomotor ability and visuomotor coordination, attention and working memory, reasoning, and executive function. Hierarchical regression analyses were used to examine predictors of cognitive performance for each cognitive measure. Possible predictors included age, gender, socioeconomic status, Wide Range Achievement Test 3 Reading score (quality of education), social networks score, hardiness score, mood disturbance score, medical problems composite score, psychoactive drug use composite score, HIV chronicity, CD4+ lymphocyte cell count, and HIV medication usage. Model 1 examined demographic factors, and model 2 examined the contribution of the remaining variables on cognitive performance. Results revealed that several factors were predictive of cognitive functioning, with the individual regression models for each measure explaining 8% to 48% of the variability in performance. Overall, this study posits that among adults with HIV, the most consistent predictors of poorer cognition included older age, poorer reading ability, more depressed mood, CD4+ lymphocyte cell count less than 200, and lack of HIV medication usage. Results suggest that those aging with HIV are subject to decreases in cognitive functioning.

The Genome Sequence of the Leaf-cutter Ant Atta Cephalotes Reveals Insights into Its Obligate Symbiotic Lifestyle

PLoS Genetics. 2011  |  Pubmed ID: 21347285

Leaf-cutter ants are one of the most important herbivorous insects in the Neotropics, harvesting vast quantities of fresh leaf material. The ants use leaves to cultivate a fungus that serves as the colony's primary food source. This obligate ant-fungus mutualism is one of the few occurrences of farming by non-humans and likely facilitated the formation of their massive colonies. Mature leaf-cutter ant colonies contain millions of workers ranging in size from small garden tenders to large soldiers, resulting in one of the most complex polymorphic caste systems within ants. To begin uncovering the genomic underpinnings of this system, we sequenced the genome of Atta cephalotes using 454 pyrosequencing. One prediction from this ant's lifestyle is that it has undergone genetic modifications that reflect its obligate dependence on the fungus for nutrients. Analysis of this genome sequence is consistent with this hypothesis, as we find evidence for reductions in genes related to nutrient acquisition. These include extensive reductions in serine proteases (which are likely unnecessary because proteolysis is not a primary mechanism used to process nutrients obtained from the fungus), a loss of genes involved in arginine biosynthesis (suggesting that this amino acid is obtained from the fungus), and the absence of a hexamerin (which sequesters amino acids during larval development in other insects). Following recent reports of genome sequences from other insects that engage in symbioses with beneficial microbes, the A. cephalotes genome provides new insights into the symbiotic lifestyle of this ant and advances our understanding of host-microbe symbioses.

Pharmacokinetics and Pharmacodynamics of Posaconazole for Invasive Pulmonary Aspergillosis: Clinical Implications for Antifungal Therapy

The Journal of Infectious Diseases. May, 2011  |  Pubmed ID: 21357943

Posaconazole is a triazole with anti-Aspergillus activity. However, little is known about the utility of posaconazole as primary therapy for invasive pulmonary aspergillosis.

The Discovery of Thyroid Replacement Therapy. Part 3: A Complete Transformation

Journal of the Royal Society of Medicine. Mar, 2011  |  Pubmed ID: 21357978

Repair of Parastomal Hernias with Biologic Grafts: a Systematic Review

Journal of Gastrointestinal Surgery : Official Journal of the Society for Surgery of the Alimentary Tract. Jul, 2011  |  Pubmed ID: 21360207

Biologic grafts are increasingly used instead of synthetic mesh for parastomal hernia repair due to concerns of synthetic mesh-related complications. This systematic review was designed to evaluate the use of these collagen-based scaffolds for the repair of parastomal hernias.

Microdosimetric Comparison of Scanned and Conventional Proton Beams Used in Radiation Therapy

Radiation Protection Dosimetry. Feb, 2011  |  Pubmed ID: 21362697

Multiple groups have hypothesised that the use of scanning beams in proton therapy will reduce the neutron component of secondary radiation in comparison with conventional methods with a corresponding reduction in risks of radiation-induced cancers. Loma Linda University Medical Center (LLUMC) has had FDA marketing clearance for scanning beams since 1988 and an experimental scanning beam has been available at the LLUMC proton facility since 2001. The facility has a dedicated research room with a scanning beam and fast switching that allows its use during patient treatments. Dosimetric measurements and microdosimetric distributions for a scanned beam are presented and compared with beams produced with the conventional methods presently used in proton therapy.

Supporting Patients with Enterocutaneous Fistula: from Hospital to Home

British Journal of Community Nursing. Feb, 2011  |  Pubmed ID: 21378670

The aim of this article is to discuss the care of patients with enterocutaneous fistula (ECF) requiring long-term support in the community. The discussion of what ECF are and aspects of nutrition will support the knowledge required to care for this group of patients effectively in their homes. This article focuses on the management of ECF appliances and gives a basic guide of skin care and how to reduce the prevalence of appliance leaks.

Contextual Factors Influencing Success or Failure of Emergency Department Interventions for Cognitively Impaired Older People: a Scoping and Integrative Review

Journal of Advanced Nursing. Jul, 2011  |  Pubmed ID: 21382076

This paper is a report of a scoping review of research on cognitive impairment in older adults who visit Emergency Departments of acute care hospitals, followed by an integrative review that included a quality assessment to determine the effectiveness of interventions for this population.

The Effects of Drug-prevention Messages on the Accessibility of Identity-related Constructs

Journal of Health Communication. May, 2011  |  Pubmed ID: 21271426

Recent theoretical work has posited that the self-system guides behavior via currently activated self-concepts. The authors adopted this framework to the study of drug-prevention messages by examining the extent to which messages can alter the accessibility of views of self and of drugs that would support nonuse. Participants were exposed to 1 of 3 print-ad conditions: autonomy-themed prevention messages (treatment), health-information themed prevention messages (comparison), and informational consumer ads (control). Outcomes were reaction times to make dichotomous judgments. Relative to comparison and control ads, treatment ads were more successful at activating a self-view as a nonuser, a view that marijuana use is inconsistent with autonomy, and unwillingness to use marijuana. Post-hoc analysis revealed that the effect of ad condition on unwillingness was partially mediated by the accessibility of self-view as a nonuser.

PH-responsive Endosomolytic Pseudo-peptides for Drug Delivery to Multicellular Spheroids Tumour Models

Biomaterials. Apr, 2011  |  Pubmed ID: 21272931

Endosomolytic polymers can aid in the endosomal release of therapeutics to improve intracellular drug delivery. pH-responsive biomimetic pseudo-peptides were synthesised by grafting l-phenylalanine onto the pendant carboxylic acids of a polyamide, poly(l-lysine isophthalamide). PP-75 (stoichiometric l-phenylalanine grafting of 75 mol%) was determined to have the best endosomolytic property. The mean hydrodynamic size of PP-75 decreased with lower pH as the polymers adopted a more compact conformation due to protonation of acidic groups and increase in hydrophobicity. PP-75 was demonstrated to deliver model drugs effectively in three dimensional (3D) magnetic HeLa multicellular spheroids used as in vitro tumour models. These spheroids can be isolated easily and quickly by magnetic separation. Due to its relatively small size, PP-75 was able to penetrate from the exterior to the interior of these spheroids and was internalised by the cells in the spheroids. It could retain its pH-mediated membrane-lytic capability in 3D drug delivery by releasing internalised calcein from intracellular endosomes in the tumour models. Furthermore, cell viability results suggest that PP-75 showed no significant cytotoxicity towards cells in the spheroids. The pH-responsive PP-75 can potentially enhance the extracellular and intracellular delivery of therapeutics in tumours.

The Discovery of Thyroid Replacement Therapy. Part 2: the Critical 19th Century

Journal of the Royal Society of Medicine. Feb, 2011  |  Pubmed ID: 21282795

Multisensory Stimulation Can Induce an Illusion of Larger Belly Size in Immersive Virtual Reality

PloS One. 2011  |  Pubmed ID: 21283823

Body change illusions have been of great interest in recent years for the understanding of how the brain represents the body. Appropriate multisensory stimulation can induce an illusion of ownership over a rubber or virtual arm, simple types of out-of-the-body experiences, and even ownership with respect to an alternate whole body. Here we use immersive virtual reality to investigate whether the illusion of a dramatic increase in belly size can be induced in males through (a) first person perspective position (b) synchronous visual-motor correlation between real and virtual arm movements, and (c) self-induced synchronous visual-tactile stimulation in the stomach area.

Differential Expression of Sclerostin in Adult and Juvenile Mouse Calvariae

Plastic and Reconstructive Surgery. Feb, 2011  |  Pubmed ID: 21285764

An understanding of the molecular mechanisms controlling bone formation is central to skeletal tissue engineering efforts. The observation that immature animals are able to heal calvarial defects while adult animals are not has proven to be a useful tool for examining these mechanisms. Thus, the authors compared expression of sclerostin, a bone inhibitor, between the calvariae of juvenile and adult mice.

Musculoskeletal Comorbidities in Cardiovascular Disease, Diabetes and Respiratory Disease: the Impact on Activity Limitations; a Representative Population-based Study

BMC Public Health. 2011  |  Pubmed ID: 21291555

The purpose of this study was to quantify the contribution of comorbidity to activity limitations in populations with chronic cardiovascular disease, diabetes or respiratory disease (index conditions), with emphasis on musculoskeletal comorbidity (arthritis or back problems).

Fast Cation-exchange Separation of Proteins in a Plastic Microcapillary Disc

Journal of Chromatography. A. Mar, 2011  |  Pubmed ID: 21292274

A novel disposable adsorbent material for fast cation-exchange separation of proteins was developed based on plastic microcapillary films (MCFs). A MCF containing 19 parallel microcapillaries, each with a mean internal diameter of 142 μm, was prepared using a melt extrusion process from an ethylene-vinyl alcohol copolymer (EVOH). The MCF was surface functionalized to produce a cation-exchange adsorbent (herein referred as MCF-EVOH-SP). The dynamic binding capacity of the new MCF-EVOH-SP material was experimentally determined by frontal analysis using pure protein solutions in a standard liquid chromatography instrument for a range of superficial flow velocities, u(LS)=5.5-27.7 cm s⁻¹. The mean dynamic binding capacity for hen-egg lysozyme was found to be approximately 100 μg for a 5 m length film, giving a ligand binding density of 413 ng cm⁻². The dynamic binding capacity did not vary significantly over the range of u(LS) tested. The application of this novel material to subtractive chromatography was demonstrated for anionic BSA and cationic lysozyme at pH 7.2. The chromatographic separation of two cationic proteins, lysozyme and cytochrome-c, was also performed with a view to applying this technology to the analysis or purification of proteins. Future applications might include separation based on anion exchange and other modes of adsorption.

Lead Levels in Eurasian Otters Decline with Time and Reveal Interactions Between Sources, Prevailing Weather, and Stream Chemistry

Environmental Science & Technology. Mar, 2011  |  Pubmed ID: 21294545

The uptake of contaminants by biota varies spatially and temporally due to a complex range of interacting environmental variables, but such complexities are typically disregarded in studies of temporal change. Here, we use linear modeling to explore spatial and temporal variation in bone Pb levels measured in samples taken from 329 Eurasian otters (Lutra lutra) found dead in southwest England. Between 1992 and 2004 Pb levels in otters fell by 73%, following UK legislative control of Pb emissions implemented since the mid 1980s. Spatial variation in bone Pb was positively correlated with modeled Pb emissions and stream sediment Pb, which interacted negatively with wind-speed and sediment Ca, respectively. Opportunistic collection of samples from wildlife mortalities provided a valuable opportunity for monitoring environmental contamination, interpretation of which was aided by spatially explicit analysis of environmental variables.

Diagnostic Criteria for Unicystic Ameloblastoma: Ameloblastic Versus Ameloblastomatous Epithelium

Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics. May, 2011  |  Pubmed ID: 21306924

Using Differential Reinforcement to Improve Equine Welfare: Shaping Appropriate Truck Loading and Feet Handling

Behavioural Processes. Mar, 2011  |  Pubmed ID: 21310219

Inappropriate behavior during common handling procedures with horses is often subject to aversive treatment. The present study replicated and extended previous findings using differential reinforcement to shape appropriate equine handling behavior. In Study 1, a multiple baseline across subjects design was used with four horses to determine first the effects of shaping target-touch responses and then successive approximations of full truck loading under continuous and intermittent schedules of reinforcement. Full loading responses were shaped and maintained in all four horses and occurrences of inappropriate behaviors reduced to zero. Generalization of the loading response was also observed to both a novel trainer and trailer. In Study 2, a changing criterion design was used to increase the duration of feet handling with one horse. The horse's responding reached the terminal duration criterion of 1min and showed consistent generalization and one-week maintenance. Overall, the results of both studies support the use of applied equine training systems based on positive reinforcement for increasing appropriate behavior during common handling procedures.

Predictive Validity of a Non-induced Mouse Model of Compulsive-like Behavior

Behavioural Brain Research. Aug, 2011  |  Pubmed ID: 21316394

A key to advancing the understanding of obsessive-compulsive disorder (OCD)-like symptoms is the development of spontaneous animal models. Over 55 generations of bidirectional selection for nest-building behavior in house mice, Mus musculus, resulted in a 40-fold difference in the amount of cotton used for a nest in high (BIG) and low (SMALL) selected lines. The nesting behavior of BIG mice appears to be compulsive-like and has initial face validity as an animal model for OCD in humans. Compulsive-like digging behavior was assessed; BIG male mice buried about three times as many marbles as SMALL male mice, strengthening face validity. Using the open field and elevated plus maze, SMALL male mice showed higher levels of anxiety/fear-like behavior than BIG male mice, indicating that compulsive-like and not anxiety-like behavior was measured. To establish predictive validity, chronic (4 weeks) oral administration of fluoxetine (30, 50 and 100mg/kg/day) and clomipramine (80 mg/kg/day), both effective in treating OCD, significantly reduced compulsive-like nest-building behavior in BIG male mice. Compulsive-like digging behavior was also significantly reduced by chronic oral fluoxetine (30 and 80 mg/kg/day) treatment in BIG male mice. General locomotor activity was not affected by chronic oral fluoxetine (30 and 80 mg/kg/day) treatment; chronic oral treatment with desipramine (30 mg/kg/day), an antidepressant not effective in treating OCD, had no effect on nesting behavior of BIG male mice, strengthening predictive validity. Together, the results indicate that these mice have good face and predictive validity as a non-induced mouse model of compulsive-like behavior relevant to OCD.

Search for Dilepton Resonances in Pp Collisions at ˆ�s=7 TeV with the ATLAS Detector

Physical Review Letters. Dec, 2011  |  Pubmed ID: 22243306

This Letter reports on a search for narrow high-mass resonances decaying into dilepton final states. The data were recorded by the ATLAS experiment in pp collisions at √s=7  TeV at the Large Hadron Collider and correspond to a total integrated luminosity of 1.08 (1.21)  fb(-1) in the e(+)e(-) (μ(+)μ(-)) channel. No statistically significant excess above the standard model expectation is observed and upper limits are set at the 95% C.L. on the cross section times branching fraction of Z' resonances and Randall-Sundrum gravitons decaying into dileptons as a function of the resonance mass. A lower mass limit of 1.83 TeV on the sequential standard model Z' boson is set. A Randall-Sundrum graviton with coupling k/M(Pl)=0.1 is excluded at 95% C.L. for masses below 1.63 TeV.

Early Postoperative Outcomes and Medication Cost Savings After Laparoscopic Sleeve Gastrectomy in Morbidly Obese Patients with Type 2 Diabetes

Journal of Obesity. 2011  |  Pubmed ID: 22187636

Background. We investigated the effect of laparoscopic sleeve gastrectomy (LSG) on morbidly obese diabetics and examined the short-term impact of LSG on diabetic medication cost. Methods. A prospective database of consecutive bariatric patients was reviewed. Morbidly obese patients with type 2 diabetes who underwent LSG were included in the study. Age, gender, body mass index (BMI), diabetic medication use, glucose, insulin, and HbA1c levels were documented preoperatively, and at 2 weeks, 2 months, 6 months, and 12 months postoperatively. Insulin resistance was estimated using the homeostatic model assessment (HOMA). Use and cost of diabetic medications were followed. Results. Of 178 patients, 22 were diabetics who underwent LSG. Diabetes remission was observed in 62% of patients within 2 months and in 75% of patients within 12 months. HOMA-IR improved after only two weeks following surgery (16.5 versus 6.6, P < 0.001). Average number of diabetic medications decreased from 2.2 to <1, within 2 weeks after surgery; corresponding to a diabetes medication cost savings of 80%, 91%, 99%, and 99.7% after 2 weeks, 2 months, 6 months, and 12 months, respectively. Conclusion. Morbidly obese patients with diabetes who undergo LSG have high rates of diabetes remission early after surgery. This translates to a significant medication cost savings.

Initial Parathyroid Surgery in 606 Patients with Renal Hyperparathyroidism

World Journal of Surgery. Feb, 2012  |  Pubmed ID: 22202993

The aim of the present study was to evaluate the outcome of different surgical procedures for patients on permanent dialysis who underwent initial parathyroidectomy for renal hyperparathyroidism (rHPT).

Clinical Impact of TP53 Alterations in Adrenocortical Carcinomas

Langenbeck's Archives of Surgery / Deutsche Gesellschaft Für Chirurgie. Feb, 2012  |  Pubmed ID: 22203015

To evaluate the role of somatic TP53 mutations and to correlate somatic and germline mutations with results of immunostaining, a large cohort of ACC patients was analyzed.

The Impact of State Safe Routes to School-related Laws on Active Travel to School Policies and Practices in U.S. Elementary Schools

Health & Place. Jan, 2012  |  Pubmed ID: 22243902

This study examined the relationship between state laws requiring minimum bussing distances, hazardous route exemptions, sidewalks, crossing guards, speed zones, and traffic control measures around schools and active travel to school (ATS) policies/practices in nationally representative samples of U.S. public elementary schools between 2007-2009. The state laws and school data were compiled through primary legal research and annual mail-back surveys of principals, respectively. Multivariate logistic and zero-inflated poisson regression indicated that all state law categories (except for sidewalks) relate to ATS. These laws should be considered in addition to formal safe routes to school programs as possible influences on ATS.

To Be Looked at As Though Air: Civil Attention Matters

Psychological Science. Feb, 2012  |  Pubmed ID: 22246319

Structural Analysis of the Plasmodium Falciparum Erythrocyte Membrane Protein 1 (PfEMP1) Intracellular Domain Reveals a Conserved Interaction Epitope

The Journal of Biological Chemistry. Jan, 2012  |  Pubmed ID: 22249178

P. falciparum infected red blood cells adhere to endothelial cells, thereby obstructing the microvasculature. Erythrocyte adherence is directly associated with severe malaria and increased disease lethality, and it is mediated by the PfEMP1 family. PfEMP1 clustering in knob-like protrusions on the erythrocyte membrane is critical for cytoadherence, however the molecular mechanisms behind this system remain elusive. Here, we show that the intracellular domains of the PfEMP1 family (ATS) share a unique molecular architecture, which comprises a minimal folded core and extensive flexible elements. A conserved flexible segment at the ATS centre is minimally restrained by the folded core. Yeast-two-hybrid data and a novel sequence analysis method suggest that this central segment contains a conserved protein interaction epitope. Interestingly, ATS in solution fails to bind the parasite knob-associated histidine rich protein (KAHRP), an essential cytoadherence component. Instead, we demonstrate that ATS associates with PFI1780w, a member of the Plasmodium helical interspersed sub-telomeric (PHIST) family. PHIST domains are widespread in exported parasite proteins, however this is the first specific molecular function assigned to any variant of this family. We propose that PHIST domains facilitate protein interactions, and that the conserved ATS epitope may be targeted to disrupt the parasite cytoadherence system.

A Systematic Review of RIFLE Criteria in Children, and Its Application and Association with Measures of Mortality and Morbidity

Kidney International. Jan, 2012  |  Pubmed ID: 22258324

The RIFLE criteria were developed to improve consistency in the assessment of acute kidney injury. The high face validity, collaborative development method, and validation against mortality have supported the widespread adoption of the RIFLE to evaluate adult patients; however, its inconsistent application in adult studies is associated with significant effects on the estimated incidence of acute kidney injury. As the RIFLE criteria are now being used to determine acute kidney injury in children, we conducted a systematic review to describe its application and assess associations between the RIFLE and measures of mortality and morbidity in pediatric patients. In 12 studies we found wide variation in the application of the RIFLE, including the range of assessed RIFLE categories, omission of urine output criteria, varying definitions of baseline renal function, and methods for handling missing baseline measurements. Limited and conflicting associations between the RIFLE and mortality, length of stay, illness severity, and measures of kidney function were found. Thus, although the RIFLE was developed to improve the consistency of defining acute kidney injury, there are still major discrepancies in its use in pediatric patients that may undermine its potential utility as a standardized measure of acute kidney injury in children.Kidney International advance online publication, 18 January 2012; doi:10.1038/ki.2011.466.

[Estimate of Corrected Values and the Effect of Correction for Measurement Error in Dietary Data Obtained by the Food Frequency Questionnaire for Adolescents (AFFQ)]

Ciência & Saúde Coletiva. Feb, 2012  |  Pubmed ID: 22267040

The scope of this study was to estimate calibrated values for dietary data obtained by the Food Frequency Questionnaire for Adolescents (FFQA) and illustrate the effect of this approach on food consumption data. The adolescents were assessed on two occasions, with an average interval of twelve months. In 2004, 393 adolescents participated, and 289 were then reassessed in 2005. Dietary data obtained by the FFQA were calibrated using the regression coefficients estimated from the average of two 24-hour recalls (24HR) of the subsample. The calibrated values were similar to the the 24HR reference measurement in the subsample. In 2004 and 2005 a significant difference was observed between the average consumption levels of the FFQA before and after calibration for all nutrients. With the use of calibrated data the proportion of schoolchildren who had fiber intake below the recommended level increased. Therefore, it is seen that calibrated data can be used to obtain adjusted associations due to reclassification of subjects within the predetermined categories.

A Polar Corundum Oxide Displaying Weak Ferromagnetism at Room Temperature

Journal of the American Chemical Society. Feb, 2012  |  Pubmed ID: 22280499

Combining long-range magnetic order with polarity in the same structure is a prerequisite for the design of (magnetoelectric) multiferroic materials. There are now several demonstrated strategies to achieve this goal, but retaining magnetic order above room temperature remains a difficult target. Iron oxides in the +3 oxidation state have high magnetic ordering temperatures due to the size of the coupled moments. Here we prepare and characterize ScFeO(3) (SFO), which under pressure and in strain-stabilized thin films adopts a polar variant of the corundum structure, one of the archetypal binary oxide structures. Polar corundum ScFeO(3) has a weak ferromagnetic ground state below 356 K-this is in contrast to the purely antiferromagnetic ground state adopted by the well-studied ferroelectric BiFeO(3).

Implementation of a Comprehensive Blood Conservation Program Can Reduce Blood Use in a Community Cardiac Surgery Program

The Journal of Thoracic and Cardiovascular Surgery. Jan, 2012  |  Pubmed ID: 22285327

OBJECTIVE: The study objective was to determine the effects of implementing a blood conservation algorithm on blood product use and outcomes in a community cardiac surgery program. METHODS: A blood management strategy including lower hemoglobin transfusion threshold and algorithm-driven decisions was adopted. Intraoperatively, point-of-care testing was used to avoid inappropriate component transfusion. A low prime perfusion circuit was adopted. Blood was withdrawn from patients before initiating bypass when possible. Patients undergoing coronary and valve procedures were included. Outlier patients receiving more than 10 units packed red blood cells were excluded. Data were collected for 6 months as a baseline group (group I). A 3-month period of program implementation was allotted. Data were subsequently collected for 6 months and comprised the study patients (group II). Prospective data were collected on demographics, blood use, and outcomes. RESULTS: Group I comprised 481 patients, and group II comprised 551 patients. Group II received fewer units of packed red blood cells, fresh-frozen plasma, and cryoprecipitate than group I. There was no difference in platelets transfused. Total blood product use was reduced by 40% in group II (P < .001). The overall 30-day mortality was 1.3%. There were no differences in mortality, reoperation for bleeding, or other postoperative outcomes between the groups. CONCLUSIONS: Implementation of a comprehensive blood conservation algorithm can be rapidly introduced, leading to reductions in blood and component use with no detrimental effect on early outcomes. Point-of-care testing can direct component transfusion in coagulopathic cases, with most coagulopathic patients requiring platelets. Further research will determine the effects of reduced transfusions on long-term outcomes.

Multi-modal Pain Measurements in Infants

Journal of Neuroscience Methods. Jan, 2012  |  Pubmed ID: 22285660

A non-invasive integrated method was developed to measure neural and behavioural responses to peripheral sensory and noxious stimulation in human infants. The introduction of a novel event-detection interface allows synchronous recording of: (i) muscle and central nervous system activity with surface electromyography (EMG), scalp electroencephalography (EEG) and near-infrared spectroscopy (NIRS); (ii) behavioural responses with video-recording and (iii) autonomic responses (heart rate, oxygen saturation, respiratory rate and cardiovascular activity) with electrocardiography (ECG) and pulse oximetry. The system can detect noxious heel lance and touch stimuli with precision (33μs and 624μs respectively) and accuracy (523μs and 256μs) and has 100% sensitivity and specificity for both types of stimulation. Its ability to detect response latencies accurately was demonstrated by a shift in latency of the vertex potential of 20.7±15.7ms (n=6 infants), following touch of the heel and of the shoulder, reflecting the distance between the two sites. This integrated system has provided reliable and reproducible measurements of responses to sensory and noxious stimulation in human infants on more than 100 test occasions.

Treatment of Helicobacter Pylori in Latin America

Lancet. Feb, 2012  |  Pubmed ID: 22305216

Dutasteride for the Treatment of Prostate-related Conditions

Expert Opinion on Drug Safety. Feb, 2012  |  Pubmed ID: 22316171

Introduction: A variety of pharmaceuticals have been developed directed at mitigating the symptoms associated with benign prostatic hypertrophy (BPH) and have also been evaluated for their potential role in prevention and treatment of prostate cancer. One such agent is dutasteride , a non-selective inhibitor of 5α-reductase, an enzyme responsible for conversion of testosterone to a more potent androgen dihydrotestosterone (DHT). Areas covered: This review will cover the safety profile of dutasteride when it is used in the treatment of prostate-related conditions, specifically looking at the pivotal clinical trials on this drug. Expert opinion: Dutasteride has proved to be a safe and efficacious treatment for symptoms related to BPH. The primary safety concern relates to the increased incidence of high-grade prostate cancer seen in men treated with dutasteride in the setting of prostate cancer prevention. Dutasteride has a role as an adjunct in the treatment of prostate cancer; however, this is an area still under active investigation. It is not recommended for use in prostate cancer prevention given the increased risk of high-grade cancers.

Physician Burnout and Patient Satisfaction with Consultation in Primary Health Care Settings: Evidence of Relationships from a One-with-many Design

Journal of Clinical Psychology in Medical Settings. Feb, 2012  |  Pubmed ID: 22327237

Physician burnout, as a prolonged response to chronic emotional and interpersonal stressors on the job, has been associated with suboptimal patient care and deterioration in the patient-provider relationship. Although prior studies have identified a range of factors associated with decreased patient satisfaction, most have been conducted in tertiary care settings, with staff burnout examined at the hospital unit-level. To examine the impact of physician burnout on patient satisfaction from consultation in the primary care setting, a cross-sectional survey was conducted in Western Greece. Using a one-with-many design, 30 physicians and 300 of their patients, randomly selected, responded to the survey. Results showed that patient satisfaction correlated significantly with physician emotional exhaustion (r = -.636, p < .01) and physician depersonalization (r = -.541, p < .01). Mixed-effects multilevel models indicated that 34.4% of total variation in patients' satisfaction occurred at the physician level, after adjustment for patients' characteristics. Moreover, physician emotional exhaustion and depersonalization remained significant factors associated with patient satisfaction with consultation, after controlling for patient and physician characteristics. Patients of physicians with high-exhaustion and high-depersonalization had significantly lower satisfaction scores, compared with patients of physicians with low-exhaustion and low-depersonalization, respectively. Future studies need to explore the mechanisms by which physician burnout affects patient satisfaction.

Associations Between Cerebrospinal Fluid Biomarkers and Long-term Neurologic Outcome in Dogs with Acute Intervertebral Disk Herniation

Journal of the American Veterinary Medical Association. Mar, 2012  |  Pubmed ID: 22332624

Objective-To examine associations between CSF biomarkers, initial neurologic dysfunction, and long-term ambulatory outcome in dogs with acute intervertebral disk herniation (IVDH). Design-Prospective clinical study. Animals-54 dogs with acute thoracolumbar IVDH and 16 clinically normal dogs. Procedures-For each dog, variables, including CSF myelin basic protein (MBP), lactate, calcium, glucose, and total protein concentrations; nucleated cell count; and creatine kinase (CK) and aspartate aminotransferase activities, were measured. For dogs with thoracolumbar IVDH, initial neurologic function was characterized by use of a modified Frankel score (MFS; determined on a scale of 0 to 5, where 0 represented paraplegia with no deep nociception and 5 represented paraspinal hyperesthesia only). Long-term follow-up was assessed. Results-Among dogs with thoracolumbar IVDH, those with CSF CK activity ≤ 38 U/L had a 35-fold increase in the odds of long-term ambulation, compared with the odds in dogs with CSF CK activity > 38 U/L, adjusting for neurologic functioning at the evaluation. The CSF lactate, calcium, and glucose concentrations and aspartate aminotransferase activity were not predictive of long-term ambulatory outcome. Data analysis revealed that long-term functional recovery was > 98% for affected dogs, regardless of their initial MFS, when CSF CK activity was ≤ 38 U/L and MBP concentration was ≤ 3 ng/mL. Conclusions and Clinical Relevance-In dogs with acute thoracolumbar IVDH, CSF CK activity and MBP concentration appeared to be prognostic indicators and, along with initial MFS, can be used to predict long-term ambulatory outcome.

Increasing Support for Alcohol-Control Enforcement Through News Coverage of Alcohol's Role in Injuries and Crime

Journal of Studies on Alcohol and Drugs. Mar, 2012  |  Pubmed ID: 22333339

ABSTRACT. Objective: Prior research has shown that the proportion of news stories about violent crimes, car crashes, and other unintended injuries that mention the possible contributing role of alcohol is far lower than the actual proportion of alcohol-related crimes and unintended injuries. An experiment was conducted to test the hypothesis that inclusion of such mention can increase concern about alcohol risks and support for alcohol-control measures, which have elsewhere been shown to decrease alcohol-related problems in community settings. Methodologically, we provide a model for experiments permitting generalization across randomly selected message stimuli. Method: Sixty randomly selected local news stories on violent crime, motor vehicle crashes, and other unintended injuries from newspapers throughout the United States were manipulated into versions including or not including alcohol as a causative factor. Participants (n = 785) were drawn from a national online research panel representative of the U.S. population; 66% of panel members contacted agreed to participate. Data were analyzed using mixed-effect, multilevel models to permit generalization across message and participant variability. Results: Mention of alcohol in news stories increased support for enforcement of alcohol-control laws. Conclusions: Efforts to increase mention of alcohol as a causative factor in news reports of violent crime and unintended injury have the potential to increase public support for alcohol-control policies. (J. Stud. Alcohol Drugs, 73, 311-315, 2012).

Mumps Outbreak in Israel's Highly Vaccinated Society: Are Two Doses Enough?

Epidemiology and Infection. Mar, 2012  |  Pubmed ID: 21554780

SUMMARYMumps outbreaks in recent years have given rise to questions about the effectiveness of the mumps vaccine. This study examined the epidemiological data from a recent mumps outbreak in Israel and from outbreaks in other countries with high vaccination coverage, and considered whether long-established vaccination policies designed to protect against mumps are in need of revision. Of over 5000 case patients in the Israeli outbreak, half of whom were in the Jerusalem health district, nearly 40% were aged ⩾15 years and, of those whose vaccination status was known, 78% had been fully vaccinated for their age - features similar to those in recent mumps outbreaks in Europe and North America. The epidemiological and laboratory evidence suggests that many previously vaccinated adolescents and young adults are now susceptible to mumps because their vaccine-based immunity has waned. Booster vaccination programmes for those at high risk of infection during mumps outbreaks - particularly those in congregate living environments - merit priority consideration.

Behavioral Consequences of Conflict-oriented Health News Coverage: the 2009 Mammography Guideline Controversy and Online Information Seeking

Health Communication. Feb, 2012  |  Pubmed ID: 21823950

Building on channel complementarity theory and media-system dependency theory, this study explores the impact of conflict-oriented news coverage of health issues on information seeking online. Using Google search data as a measure of behavior, we demonstrate that controversial news coverage of the U.S. Preventive Services Task Force's November 2009 recommendations for changes in breast cancer screening guidelines strongly predicted the volume of same-day online searches for information about mammograms. We also found that this relationship did not exist 1 year prior to the coverage, during which mammography news coverage did not focus on the guideline controversy, suggesting that the controversy frame may have driven search behavior. We discuss the implications of these results for health communication scholars and practitioners.

The Relationship Between Gabapentin and Pregabalin and Posttraumatic Stress Disorder in Burned Servicemembers

Journal of Burn Care & Research : Official Publication of the American Burn Association. Jan, 2012  |  Pubmed ID: 22210072

Posttraumatic stress disorder (PTSD) affects approximately 30% of burned Servicemembers returning from Operation Iraqi Freedom/Operation Enduring Freedom. Gabapentin and pregabalin are anticonvulsant drugs that limited evidence suggests may also be effective treatments for some psychological disorders. This study examines the relationship between these anticonvulsants and PTSD development in burned Servicemembers. Drugs received, injury severity score, TBSA burned, length of hospital stay, number of intensive care unit days, number of surgeries, and PTSD Checklist-Military scores and administration dates were collected. Subjects were grouped based on receipt of gabapentin or pregabalin, and the groups were compared. The primary outcome was incidence of a positive screen for PTSD. Because injury severity was significantly different between the two groups, propensity score matching based on injury severity score and TBSA was performed. Two hundred ninety burned Servicemembers received the PTSD Checklist-Military at least 30 days after injury. Of these subjects, 104 received gabapentin, pregabalin, or both and 186 did not. Despite significantly greater injuries, the group that received gabapentin or pregabalin did not develop PTSD at a different rate than those patients who did (P = .727). Propensity score matching resulted in 57 patients in each group; there was no difference between these groups in the incidence of PTSD (P = .663). These data suggest that gabapentin or pregabalin administration may not affect PTSD development in burned Servicemembers. Many factors influence the development and progression of PTSD, but few drugs have been identified that are effective at treating or preventing PTSD.

Fragile X Mental Retardation 1 (FMR1) Intron 1 Methylation in Blood Predicts Verbal Cognitive Impairment in Female Carriers of Expanded FMR1 Alleles: Evidence from a Pilot Study

Clinical Chemistry. Jan, 2012  |  Pubmed ID: 22235103

BACKGROUND:Cognitive status in females with mutations in the FMR1 (fragile X mental retardation 1) gene is highly variable. A biomarker would be of value for predicting which individuals were liable to develop cognitive impairment and could benefit from early intervention. A detailed analysis of CpG sites bridging exon 1 and intron 1 of FMR1, known as fragile X- related epigenetic element 2 (FREE2), suggests that a simple blood test could identify these individuals.METHODS:Study participants included 74 control females (<40 CGG repeats), 62 premutation (PM) females (55-200 CGG repeats), and 18 full-mutation (FM) females assessed with Wechsler intelligence quotient (IQ) tests. We used MALDI-TOF mass spectrometry to determine the methylation status of FREE2 CpG sites that best identified low-functioning (IQ <70) FM females (>200 CGG repeats), compared the results with those for Southern blot FMR1 activation ratios, and related these assessments to the level of production of the FMR1 protein product in blood.RESULTS:A methylation analysis of intron 1 CpG sites 10-12 showed the highest diagnostic sensitivity (100%) and specificity (98%) of all the molecular measures tested for detecting females with a standardized verbal IQ of <70 among the study participants. In the group consisting of only FM females, methylation of these sites was significantly correlated with full-scale IQ, verbal IQ, and performance IQ. Several verbal subtest scores showed strong correlation with the methylation of these sites (P = 1.2 × 10(-5)) after adjustment for multiple measures.CONCLUSIONS:The data suggest that hypermethylation of the FMR1 intron 1 sites in blood is predictive of cognitive impairment in FM females, with implications for improved fragile X syndrome diagnostics in young children and screening of the newborn population.

Toxic Stress Prioritizes and Imbalances Stem Cell Differentiation: Implications for New Biomarkers and in Vitro Toxicology Tests

Systems Biology in Reproductive Medicine. Feb, 2012  |  Pubmed ID: 22239079

This hypothesis and review introduces rules of stem cell stress responses that provide biomarkers and alternative testing that replaces or reduces gestational tests using whole animals. These rules for the stress responses of cultured stem cells validate the organismal strategy of the stress response and show that it emulates what must happen if the conceptus implants during a response to stress in vivo. Specifically there is a profound threshold during a stress dose response where stem cell accumulation is significantly reduced. Below this threshold stress enzymes manage the stress response by converting anabolic to catabolic processes and by suppressing apoptosis, without affecting differentiation. However above this threshold the stem cell survival response converts to an organismal survival response where stress enzymes switch to new substrates and mediate loss of potency factors, gain of early essential differentiated lineages, and suppression of later essential lineages. Stressed stem cells 'compensate' for lower accumulation rates by differentiating a higher fraction of cells, and the organismal survival response further enhances adaptation by prioritizing the differentiation of early essential lineages. Thus compensatory and prioritized differentiation and the sets of markers produced are part of a response of cultured embryos and stem cells that emulate what must happen during implantation of a stressed gestation. Knowledge of these markers and use of stressed stem cell assays in culture should replace or reduce the number of animals needed for developmental toxicity and should produce biomarkers for stressed development in vitro and in vivo.

Biomolecule Surface Patterning May Enhance Membrane Association

ACS Nano. Jan, 2012  |  Pubmed ID: 22239824

Under dehydration conditions, amphipathic late embryogenesis abundant proteins fold spontaneously from a random conformation into α-helical structures, and this transition is promoted by the presence of membranes. To gain insight into the thermodynamics of membrane association, we model the resulting α-helical structures as infinite rigid cylinders patterned with hydrophobic and hydrophilic stripes oriented parallel to their axis. Statistical thermodynamic calculations using single chain mean field theory show that the relative thickness of the stripes controls the free energy of interaction of the α-helices with a phospholipid bilayer, as does the bilayer structure and the depth of the equilibrium penetration of the cylinders into the bilayer. The results may suggest the optimal thickness of the stripes to mimic the association of such protein with membranes.

Rapid, Multiplexed Microfluidic Phage Display

Lab on a Chip. Feb, 2012  |  Pubmed ID: 22182980

The development of a method for high-throughput, automated proteomic screening could impact areas ranging from fundamental molecular interactions to the discovery of novel disease markers and therapeutic targets. Surface display techniques allow for efficient handling of large molecular libraries in small volumes. In particular, phage display has emerged as a powerful technology for selecting peptides and proteins with enhanced, target-specific binding affinities. Yet, the process becomes cumbersome and time-consuming when multiple targets are involved. Here we demonstrate for the first time a microfluidic chip capable of identifying high affinity phage-displayed peptides for multiple targets in just a single round and without the need for bacterial infection. The chip is shown to be able to yield well-established control consensus sequences while simultaneously identifying new sequences for clinically important targets. Indeed, the confined parameters of the device allow not only for highly controlled assay conditions but also introduce a significant time-reduction to the phage display process. We anticipate that this easily-fabricated, disposable device has the potential to impact areas ranging from fundamental studies of protein, peptide, and molecular interactions, to applications such as fully automated proteomic screening.

Effects of Daily Activities on Dual-energy X-ray Absorptiometry Measurements of Body Composition in Active People

Medicine and Science in Sports and Exercise. Jan, 2012  |  Pubmed ID: 22179140

Dual-energy x-ray absorptiometry (DXA) is becoming a popular tool to measure body composition in athletes, owing to its ease of operation and comprehensive analysis of body composition. This study represents the first systematic investigation of the reliability of DXA measurements of body composition in trained individuals and includes measurements of daily variability as well as the specific effect of the intake of a meal.

Encapsulation of Yeast Cells in Colloidosomes

Langmuir : the ACS Journal of Surfaces and Colloids. Jan, 2012  |  Pubmed ID: 22149136

Polymeric colloidosomes encapsulating viable Baker's yeast cells were prepared. To make the capsules, an aqueous suspension of 153 nm poly(methyl methacrylate-co-butyl acrylate) latex particles plus yeast cells is emulsified in a continuous phase of sunflower oil. By adding a small amount of ethanol to the oil phase, the latex particles at the surface of the emulsion droplets aggregate, forming the colloidosome shells. The microcapsules have been examined using optical, confocal, and scanning electron microscopies. The viability of the yeast cells was tested using fluorescent molecular probes. The encapsulated Baker's yeast cells were able to metabolize glucose from solution, although at a slower rate compared to nonencapsulated yeast. This demonstrates diffusion limitation through the colloidosome shell. The diffusive resistance could be increased by manufacturing colloidosomes with a double latex shell.

A Short-term Biomarker Modulation Study of Simvastatin in Women at Increased Risk of a New Breast Cancer

Breast Cancer Research and Treatment. Feb, 2012  |  Pubmed ID: 22076478

Observational studies have demonstrated a decreased incidence of cancers among users of HMG CoA reductase inhibitors (statins) and a reduced risk of recurrence among statin users diagnosed with early stage breast cancer. We initiated a prospective study to identify potential biomarkers of simvastatin chemopreventive activity that can be validated in future trials. The contralateral breast of women with a previous history of breast cancer was used as a high-risk model. Eligible women who had completed all planned treatment of a prior stage 0-III breast cancer received simvastatin 40 mg orally daily for 24-28 weeks. At baseline and end-of-study, we measured circulating concentrations of high-sensitivity C-reactive protein (hsCRP), estrogens, and fasting lipids; breast density on contralateral breast mammogram; and quality of life by Rand Short Form 36-Item health survey. Fifty women were enrolled with a median age of 53 years. Total cholesterol, LDL cholesterol, triglyceride, and hsCRP fell significantly during the study (P values < 0.001, <0.001, 0.003, and 0.05, respectively). Estrone sulfate concentrations decreased with simvastatin treatment (P = 0.01 overall), particularly among post-menopausal participants (P = 0.006). We did not observe a significant change in circulating estradiol or estrone concentrations, contralateral mammographic breast density, or reported physical functioning or pain scores. This study demonstrates the feasibility of short-term biomarker modulation studies using the contralateral breast of high-risk women. Simvastatin appears to modulate estrone sulfate concentrations and its potential chemopreventive activity in breast cancer warrants further investigation.

Triblock Copolymer Based on Poly(propylene Oxide) and Poly(1-[11-acryloylundecyl]-3-methyl-imidazolium Bromide)

Macromolecular Rapid Communications. Jan, 2012  |  Pubmed ID: 22135006

The controlled atom transfer radical polymerization of an ionic liquid, 1-(11-acryloylundecyl)-3-methyl imidazolium bromide (ILBr), from both ends of a telechelic poly(propylene oxide) (PPO) macroinitiator, end-functionalized with bromoisobutyryloyl is reported. The resulting highly water-soluble triblock, poly(ILBr-b-PO-b-ILBr) is multistimuli responsive. This new class of triblocks exhibits classical surface activity in lowering surface tension at the air-water interface and in modifying wetting in waterborne coatings. It also immunizes model colloids against coagulation induced by Debye-Hückel (indifferent electrolyte) electrostatic screening. Further, sol-gel thermoreversibility is unexpectedly found as an additional form of stimuli responsiveness.

Inflammatory Aneurysms of the Ascending Aorta: Diagnosis and Surgical Treatment

Journal of Cardiac Surgery. Jan, 2012  |  Pubmed ID: 22103645

Abstract  Inflammatory aortic aneurysms (IAAs) are rare and located mainly in the infra-renal abdominal aorta. The ascending aorta has been typically spared from this process. We report on two cases of ascending aortic inflammatory aneurysms and describe the available literature on this rare entity. (J Card Surg 2012;27:70-74).

Insight into the Local Structure of Barium Indate Oxide-ion Conductors: an X-ray Total Scattering Study

Dalton Transactions (Cambridge, England : 2003). Jan, 2012  |  Pubmed ID: 22105971

In this paper we presented the X-ray PDF investigation of orthorhombic Ba(2)In(2)O(5) and cubic Ba(2)In(1.7)P(0.3)O(5.3) and Ba(2)In(1.7)S(0.3)O(5.45) samples. Pure Ba(2)In(2)O(5) was found to be properly described-at the local scale-by the orthorhombic average structure. Ba(2)In(1.7)P(0.3)O(5.3) and Ba(2)In(1.7)S(0.3)O(5.45) cannot be described, at the local scale, by a cubic symmetry. The PDFs of these two samples clearly showed a distorted atom arrangement in the short-range which can be described again with the orthorhombic symmetry found in pure barium indate.

Inflammation in Utero Exacerbates Ventilation-induced Brain Injury in Preterm Lambs

Journal of Applied Physiology (Bethesda, Md. : 1985). Feb, 2012  |  Pubmed ID: 22052871

Cerebral blood flow disturbance is a major contributor to brain injury in the preterm infant. The initiation of ventilation may be a critical time for cerebral hemodynamic disturbance leading to brain injury in preterm infants, particularly if they are exposed to inflammation in utero. We aimed to determine whether exposure to inflammation in utero alters cardiopulmonary hemodynamics, resulting in cerebral hemodynamic disturbance and related brain injury during the initiation of ventilation. Furthermore, we aimed to determine whether inflammation in utero alters the cerebral hemodynamic response to challenge induced by high mean airway pressures. Pregnant ewes received intra-amniotic lipopolysaccharide (LPS) or saline either 2 or 4-days before preterm delivery (at 128 ± 1 days of gestation). Lambs were surgically instrumented for assessment of pulmonary and cerebral hemodynamics before delivery and positive pressure ventilation. After 30 min, lambs were challenged hemodynamically by incrementing and decrementing positive end-expiratory pressure. Blood gases, arterial pressures, and blood flows were recorded. The brain was collected for biochemical and histological assessment of inflammation, brain damage, vascular extravasation, hemorrhage, and oxidative injury. Carotid arterial pressure was higher and carotid blood flow was more variable in 2-day LPS lambs than in controls during the initial 15 min of ventilation. All lambs responded similarly to the hemodynamic challenge. Both 2- and 4-day LPS lambs had increased brain interleukin (IL)-1β, IL-6, and IL-8 mRNA expression; increased number of inflammatory cells in the white matter; increased incidence and severity of brain damage; and vascular extravasation relative to controls. Microvascular hemorrhage was increased in 2-day LPS lambs compared with controls. Cerebral oxidative injury was not different between groups. Antenatal inflammation causes adverse cerebral hemodynamics and increases the incidence and severity of brain injury in ventilated preterm lambs.

Synthesis and Characterization of Proton Conducting Oxyanion Doped Ba2Sc2O5

Dalton Transactions (Cambridge, England : 2003). Jan, 2012  |  Pubmed ID: 22012071

In this paper we report the successful synthesis of the cubic oxyanion containing perovskites, Ba(2)Sc(2-x)P(x)O(5+x) (x = 0.4, 0.5), with the samples analysed through a combination of X-ray diffraction, NMR, TGA, Raman spectroscopy and conductivity measurements. Conductivity measurements indicate a p-type contribution to the conductivity in oxidizing conditions at elevated temperatures, with evidence for proton conduction in wet atmospheres. For the latter, bulk conductivities of 5.9 × 10(-3) and 1.3 × 10(-3) S cm(-1) at 500 °C were obtained for x = 0.4 and 0.5 respectively, comparable to other perovskite proton conductors, while the stability towards CO(2) containing atmospheres was improved compared to BaCeO(3) based systems. Related Si doped systems have also been prepared, although in this case small Ba(2)SiO(4) impurities are observed. We also provide evidence to suggest that "undoped" Ba(2)Sc(2)O(5) contains carbonate groups, which accounts for its thermal instability.

Age Related Variation in Male-male Relationships in Wild Spider Monkeys (Ateles Geoffroyi Yucatanensis)

Primates; Journal of Primatology. Jan, 2012  |  Pubmed ID: 21881958

In social organizations characterized by male philopatry, social relationships between males are argued to be the strongest. Little is known about the social relationships of philopatric male spider monkeys. To address this limitation, we investigated social relationships among individually recognized wild adult male spider monkeys from two well-habituated communities in the Yucatan Peninsula, Mexico, focusing on affiliative behaviors important in regulating male social relationships, including grooming, embracing, arm-wrapping, and grappling. We examined whether behaviors were reciprocated between male partners and whether age was a factor in how the behaviors were distributed or reciprocated, by examining differences between younger adult males (<10 years) and older adult males (≥14 years). Although we found evidence that affiliative behaviors were overall reciprocated between spider monkey adult males, there were pronounced differences in the interactions depending on their relative age. Reciprocation in grooming and embraces between same-age males suggests their relationships are valuable to both partners. Among different-age dyads, younger males gave more embraces than they received, were the initiators of grappling and arm-wrapped more often than with same-age males, suggesting relationships between younger and older males are more risky. This confirms that younger males are attracted to older males, probably because they value relationships with older males more than the reverse, but they are also at risk.