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In JoVE (1)
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Articles by Rasika Jinadasa in JoVE
Härledning av thymic Lymfom T-cellinjer från Atm - / - Och P53 - / - Möss
Rasika Jinadasa, Gabriel Balmus, Lee Gerwitz, Jamie Roden, Robert Weiss, Gerald Duhamel
Department of Biomedical Sciences, Cornell University
I denna video visar vi ett protokoll för att upprätta mus thymic linjer lymfom cell. Genom att följa detta protokoll, har vi framgångsrikt etablerat flera T-cellinjer från ATM-/ - och p53-/ - möss med thymic lymfom.
Other articles by Rasika Jinadasa on PubMed
Cytolethal Distending Toxin: a Conserved Bacterial Genotoxin That Blocks Cell Cycle Progression, Leading to Apoptosis of a Broad Range of Mammalian Cell Lineages
Microbiology (Reading, England). Jul, 2011 | Pubmed ID: 21565933
Cytolethal distending toxin (CDT) is a heterotrimeric AB-type genotoxin produced by several clinically important Gram-negative mucocutaneous bacterial pathogens. Irrespective of the bacterial species of origin, CDT causes characteristic and irreversible cell cycle arrest and apoptosis in a broad range of cultured mammalian cell lineages. The active subunit CdtB has structural homology with the phosphodiesterase family of enzymes including mammalian DNase I, and alone is necessary and sufficient to account for cellular toxicity. Indeed, mammalian cells treated with CDT initiate a DNA damage response similar to that elicited by ionizing radiation-induced DNA double strand breaks resulting in cell cycle arrest and apoptosis. The mechanism of CDT-induced apoptosis remains incompletely understood, but appears to involve both p53-dependent and -independent pathways. While epithelial, endothelial and fibroblast cell lines respond to CDT by undergoing arrest of cell cycle progression resulting in nuclear and cytoplasmic distension that precedes apoptotic cell death, cells of haematopoietic origin display rapid apoptosis following a brief period of cell cycle arrest. In this review, the ecology of pathogens producing CDT, the molecular biology of bacterial CDT and the molecular mechanisms of CDT-induced cytotoxicity are critically appraised. Understanding the contribution of a broadly conserved bacterial genotoxin that blocks progression of the mammalian cell cycle, ultimately causing cell death, should assist with elucidating disease mechanisms for these important pathogens.