Articles by Rebekka A.V. Schwab in JoVE

Visualization of DNA Replication in the Vertebrate Model System DT40 using the DNA Fiber Technique

JoVE 3255 10/27/2011

Rebekka A.V. Schwab¹, Wojciech Niedzwiedz^1,2

¹Department of Molecular Oncology, Weatherall Institute of Molecular Medicine, University of Oxford, ²Institute of Genetics and Biotechnology, Faculty of Biology, University of Warsaw

DT40, a model vertebrate genetic system, provides a powerful tool to analyze protein function. Here we describe a simple method that allows qualitative analysis of parameters that influence DNA synthesis during the S-phase in DT40 cells at the single molecule level.