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In JoVE (2)
- Isolation of Human Islets from Partially Pancreatectomized Patients
- Improved Protocol For Laser Microdissection Of Human Pancreatic Islets From Surgical Specimens
Other Publications (2)
Articles by Robert Grützmann in JoVE
Isolation of Human Islets from Partially Pancreatectomized Patients
Gregor Bötticher1, Dorothèe Sturm1, Florian Ehehalt1, Klaus P. Knoch2, Stephan Kersting1, Robert Grützmann1, Gustavo B. Baretton3, Michele Solimena2, Hans D. Saeger1
1Department of GI-, Thorax- and Vascular Surgery, University Hospital Carl Gustav Carus, University of Technology Dresden, 2Molecular Diabetology, Paul Langerhans Institute Dresden, 3Department of Pathology, University Hospital Carl Gustav Carus, University of Technology Dresden
The supply of type 2 diabetic islets for research is insufficient. Here we share our protocol for isolating islets from patients undergoing partial pancreatectomy. This approach represents a unique venue for obtaining islets from type 2 diabetic and clinically matched non-diabetic subjects in adequate numbers for basic and clinical studies.
Improved Protocol For Laser Microdissection Of Human Pancreatic Islets From Surgical Specimens
Dorothée Sturm1,2, Lorella Marselli3, Florian Ehehalt1,2, Daniela Richter1, Marius Distler2, Stephan Kersting1,2, Robert Grützmann2, Krister Bokvist4, Philippe Froguel5, Robin Liechti6, Anne Jörns7, Paolo Meda8, Gustavo Bruno Baretton9, Hans-Detlev Saeger2, Anke M. Schulte10, Piero Marchetti3, Michele Solimena1
1Molecular Diabetology, Paul Langerhans Institute Dresden, 2Department of GI-, Thoracic- and Vascular Surgery, University Hospital Carl Gustav Carus, University of Technology Dresden, 3Department of Endocrinology and Metabolism, Metabolic Unit University of Pisa, 4Labs DC0522, Lilly Corporate Center, 5Genomics, Faculty of Medicine Imperial College London, 6Vital-IT, SIB Swiss Institute of Bioinformatics, 7Clinical Biochemistry, Hannover Medical School, 8Cell Physiology and Metabolism, Medical School, University of Geneva, 9Department of Pathology, University Hospital Carl Gustav Carus, University of Technology Dresden, 10R&D DIAB Division / Translational Medicine, Sanofi-Aventis
Laser microdissection is a technique that allows the recovery of selected cells from minute amounts of parenchyma. Here we describe a protocol for acquiring human pancreatic islets from surgical specimens to be used for transcriptomic studies. Our protocol improves the intrinsic autofluorescence of human beta cells, thus facilitating their collection.
Other articles by Robert Grützmann on PubMed
ADHESION-DEPENDENT F-ACTIN PATTERN IN AMOEBA PROTEUS AS A COMMON FEATURE OF AMOEBAE AND THE METAZOAN MOTILE CELLS
Cell Biology International. Sep, 1997 | Pubmed ID: 9570891
Adhesion and movement of Amoeba proteus are both dependent on the appropriate arrangement of the F-actin cytoskeleton and on the presence of the cell nucleus. In this study the F-actin organization was examined by routine FITC-phalloidin staining and confocal laser microscopy in intact amoebae and in their nucleated and anucleated fragments, at different levels of cell adherence to the substratum. In the adhering and migrating intact cells and nucleated cell fragments dot-like aggregates of F-actin are scattered over the ventral side at sites close to the substratum. In the case of de-adhesion of nucleated specimens this pattern disappears and F-actin is accumulated in the cell centre and/or dispersed in the cytoplasm. The same actin distribution, without ventral dots, is found in the anucleated fragments which usually fail to attach to the substratum. Re-adhesion of anucleated fragments, induced by a modified substratum or spontaneous, is accompanied by restoration of actin dots at the lower cell side. It is concluded that: (1) adhering specimens of A. proteus display the same dot-like actin pattern on the ventral cell side, as many metazoan motile cells; (2) organization or disorganization of this pattern may occur independently of the presence of the cell nucleus, under the control of cell adhesion to the substratum. Copyright 1998 Academic Press Limited
[Effect of Environmental Supplementation of Iodine on Infant Mortality and Growth in Children in Xinjiang, China]
Zhonghua Liu Xing Bing Xue Za Zhi = Zhonghua Liuxingbingxue Zazhi. Jun, 2002 | Pubmed ID: 12411089
To study the effect of iodine supplementation on infant mortality and growth in Xinjiang.