Articles by Ronald D. Seidel III in JoVE

Imaging Glycans in Zebrafish Embryos by Metabolic Labeling and Bioorthogonal Click Chemistry

JoVE 2686 6/06/2011

Hao Jiang¹, Lei Feng¹, David Soriano del Amo¹, Ronald D. Seidel III², Florence Marlow³, Peng Wu¹

¹Department of Biochemistry, Albert Einstein College of Medicine, Yeshiva University, ²Macromolecular Therapeutics Development Facility, Albert Einstein College of Medicine, Yeshiva University, ³Developmental and Molecular Biology, Albert Einstein College of Medicine, Yeshiva University

A click-chemistry based method that allows for the rapid, noninvasive, and robust labeling of alkyne-tagged glycans in zebrafish embryos is described. Fucosylated glycans in the enveloping layer of zebrafish embryos in the late gastrulation stage were imaged in this study.