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In JoVE (1)
Other Publications (1)
Articles by Sagar K. Gupta in JoVE
Detection and Isolation of Circulating Melanoma Cells using Photoacoustic Flowmetry
Christine M. O'Brien, Kyle Rood, Shramik Sengupta, Sagar K. Gupta, Thiago DeSouza, Aaron Cook, John A. Viator
Biological Engineering, Christopher S. Bond Life Sciences Center, Dermatology, University of Missouri
We have developed a flow cytometer using laser induced ultrasound to detect circulating melanoma cells as an early indicator of metastatic disease.
Other articles by Sagar K. Gupta on PubMed
Lasers in Surgery and Medicine. Mar, 2010 | Pubmed ID: 20333746
Circulating tumor cells have been shown to correlate positively with metastatic disease state in patients with advanced cancer. We have demonstrated the ability to detect melanoma cells in a flow system by generating and detecting photoacoustic waves in melanoma cells. This method is similar to flow cytometry, although using photoacoustics rather than fluorescence. Previously, we used piezoelectric films as our acoustic sensors. However, such films have indicated false-positive signals due to unwanted direct interactions between photons from the high laser fluence in the flow system and the film itself. We have adapted an optical detection scheme that obviates the need for piezoelectric films.